Determination of the Binding Specificity of an Integral Membrane Protein by Saturation Transfer Difference NMR:  RGD Peptide Ligands Binding to Integrin αIIbβ3†

2001 ◽  
Vol 44 (19) ◽  
pp. 3059-3065 ◽  
Author(s):  
Robert Meinecke ◽  
Bernd Meyer
Keyword(s):  
Membrane Protein ◽  
Binding Specificity ◽  
Integral Membrane Protein ◽  
Rgd Peptide ◽  
Peptide Ligands ◽  
Saturation Transfer ◽  
Integrin Αiibβ3 ◽  
Saturation Transfer Difference

scholarly journals Structure determination of an integral membrane protein at room temperature from crystalsin situ

2015 ◽  
Vol 71 (6) ◽  
pp. 1228-1237 ◽  
Author(s):  
Danny Axford ◽  
James Foadi ◽  
Nien-Jen Hu ◽  
Hassanul Ghani Choudhury ◽  
So Iwata ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Structure Determination ◽  
Room Temperature ◽  
Protein Structures ◽  
Integral Membrane Protein ◽  
Data Sets ◽  
X Ray Diffraction ◽  
Haemophilus Influenza

The structure determination of an integral membrane protein using synchrotron X-ray diffraction data collected at room temperature directly in vapour-diffusion crystallization plates (in situ) is demonstrated. Exposing the crystalsin situeliminates manual sample handling and, since it is performed at room temperature, removes the complication of cryoprotection and potential structural anomalies induced by sample cryocooling. Essential to the method is the ability to limit radiation damage by recording a small amount of data per sample from many samples and subsequently assembling the resulting data sets using specialized software. The validity of this procedure is established by the structure determination ofHaemophilus influenzaTehA at 2.3 Å resolution. The method presented offers an effective protocol for the fast and efficient determination of membrane-protein structures at room temperature using third-generation synchrotron beamlines.

scholarly journals The role of protein purification in the structure determination of an integral membrane protein

1996 ◽  
Vol 52 (a1) ◽  
pp. C141-C141
Author(s):  
G. McDermott ◽  
S. M. Prince ◽  
M. Z. Papiz ◽  
A. M. Hawthornthwaite-Lawless ◽  
A. A. Freer ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Purification ◽  
Structure Determination ◽  
Integral Membrane Protein

MEASUREMENT OF STEROID BINDING PROTEINS

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S104-S121 ◽  
Author(s):  
E. E. Baulieu ◽  
J. P. Raynaud ◽  
E. Milgrom
Keyword(s):  
Kinetic Parameters ◽  
Binding Proteins ◽  
Binding Specificity ◽  
Binding Parameters ◽  
Target Organs ◽  
Biological Mixtures ◽  
Steroid Binding Proteins ◽  
Steroid Binding ◽  
Steroid Binding Protein

ABSTRACT A brief review of the characteristics of steroid binding proteins found in the plasma and in some target organs is presented, followed by some general remarks on binding »specificity« and binding parameters. Useful techniques for measuring binding parameters at equilibrium are reported, both those which keep the equilibrium intact and those which implicate its disruption. A concept is developed according to which the determination of a specific steroid binding protein is based on the »differential dissociation« of the several steroid binding complexes present in most biological mixtures. Methods which allow determination of the kinetic parameters of the binding systems are also presented. Various representations of the binding and therefore different modes of graphic representation and calculation are discussed, including the recent »proportion graph« method.

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