Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthaseElectronic supplementary information (ESI) available: Further details of the construction of pCJR308, the fermentation of BIOT-0966 and the isolation of pentaketide lactone, 3, and figures showing the13C NMR and 1H COSY spectra of 3. See http://www.rsc.org/suppdata/ob/b3/b310740j/

Christine J. Martin; M�ire C. Timoney; Rose M. Sheridan; Steven G. Kendrew; Barrie Wilkinson; James Staunton; Peter F. Leadlay

doi:10.1039/b310740j

Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthaseElectronic supplementary information (ESI) available: Further details of the construction of pCJR308, the fermentation of BIOT-0966 and the isolation of pentaketide lactone, 3, and figures showing the13C NMR and 1H COSY spectra of 3. See http://www.rsc.org/suppdata/ob/b3/b310740j/

Organic & Biomolecular Chemistry ◽

10.1039/b310740j ◽

2003 ◽

Vol 1 (23) ◽

pp. 4144 ◽

Cited By ~ 31

Author(s):

Christine J. Martin ◽

M�ire C. Timoney ◽

Rose M. Sheridan ◽

Steven G. Kendrew ◽

Barrie Wilkinson ◽

...

Keyword(s):

Heterologous Expression ◽

Saccharopolyspora Erythraea ◽

Supplementary Information ◽

Cosy Spectra

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Probing the effect of a 3′-S-phosphorothiolate link on the conformation of a DNA:RNA hybrid; implications for antisense drug designElectronic supplementary information (ESI) available: diagram showing sulfur link, UV melting curves, sample PE-COSY spectra, further NOE intensity data. See: http://www.rsc.org/suppdata/cc/b2/b203582k/

Chemical Communications ◽

10.1039/b203582k ◽

2002 ◽

pp. 1458-1459 ◽

Cited By ~ 16

Author(s):

Andrew P. G. Beevers ◽

Kevin J. Fettes ◽

Ian A. O’Neil ◽

Stanley M. Roberts ◽

John R. P. Arnold ◽

...

Keyword(s):

Supplementary Information ◽

Intensity Data ◽

Melting Curves ◽

Uv Melting ◽

Cosy Spectra

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Cloning, characterization, and heterologous expression of the Saccharopolyspora erythraea (Streptomyces erythraeus) gene encoding an EF-hand calcium-binding protein.

Journal of Bacteriology ◽

10.1128/jb.171.10.5614-5619.1989 ◽

1989 ◽

Vol 171 (10) ◽

pp. 5614-5619 ◽

Cited By ~ 19

Author(s):

D G Swan ◽

J Cortes ◽

R S Hale ◽

P F Leadlay

Keyword(s):

Heterologous Expression ◽

Calcium Binding ◽

Binding Protein ◽

Calcium Binding Protein ◽

Saccharopolyspora Erythraea ◽

Gene Encoding ◽

Ef Hand

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Synthesis of four derivatives of 3,6,10-tri(carboxymethyl)-3,6,10-triazadodecanedioic acid, the stabilities of their complexes with Ca(ii), Cu(ii), Zn(ii) and lanthanide(iii) and water-exchange investigations of Gd(iii) chelatesElectronic supplementary information (ESI) available: tables of pM values and relaxivities; potentiometric titration curves for TTDA-PY, TTDA-HP, TTDA-H1P and TTDA-H2P; species distribution curves, plots of temperature dependence of transverse electronic relaxation rates and plots of pH dependence of conditional stability constants for Gd complexes of all four ligands and TTDA; 1H NMR and COSY spectra of [La(TTDA-HP)]â€“. See http://www.rsc.org/suppdata/dt/b1/b107456n/

Journal of the Chemical Society Dalton Transactions ◽

10.1039/b107456n ◽

2001 ◽

pp. 3357-3366 ◽

Cited By ~ 15

Author(s):

Tsan-Hwang Cheng ◽

Yun-Ming Wang ◽

Kuei-Tang Lin ◽

Gin-Chung Liu

Keyword(s):

Water Exchange ◽

Ph Dependence ◽

Supplementary Information ◽

Electronic Relaxation ◽

Conditional Stability ◽

Relaxation Rates ◽

Titration Curves ◽

Conditional Stability Constants ◽

Derivatives Of ◽

Cosy Spectra

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High Level of Spinosad Production in the Heterologous Host Saccharopolyspora erythraea

Applied and Environmental Microbiology ◽

10.1128/aem.00618-16 ◽

2016 ◽

Vol 82 (18) ◽

pp. 5603-5611 ◽

Cited By ~ 14

Author(s):

Jun Huang ◽

Zhen Yu ◽

Mei-Hong Li ◽

Ji-Dong Wang ◽

Hua Bai ◽

...

Keyword(s):

Heterologous Expression ◽

Gene Cluster ◽

Gene Clusters ◽

Industrial Applications ◽

Global Market ◽

Saccharopolyspora Erythraea ◽

Market Demand ◽

Saccharopolyspora Spinosa ◽

Biosynthesis Gene Cluster ◽

Content Type

ABSTRACTSpinosad, a highly effective insecticide, has an excellent environmental and mammalian toxicological profile. Global market demand for spinosad is huge and growing. However, after much effort, there has been almost no improvement in the spinosad yield from the original producer,Saccharopolyspora spinosa. Here, we report the heterologous expression of spinosad usingSaccharopolyspora erythraeaas a host. The native erythromycin polyketide synthase (PKS) genes inS. erythraeawere replaced by the assembled spinosad gene cluster through iterative recombination. The production of spinosad could be detected in the recombinant strains containing the whole biosynthesis gene cluster. Both metabolic engineering and UV mutagenesis were applied to further improve the yield of spinosad. The final strain, AT-ES04PS-3007, which could produce spinosad with a titer of 830 mg/liter, has significant potential in industrial applications.IMPORTANCEThis work provides an innovative and promising way to improve the industrial production of spinosad. At the same time, it also describes a successful method of heterologous expression for target metabolites of interest by replacing large gene clusters.

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