Substrate turnover and inhibitor binding as selection parameters in directed evolution of blood coagulation factor Xa

2003 ◽  
Vol 1 (10) ◽  
pp. 1657-1663 ◽  
Author(s):  
Rikke H. Lorentsen ◽  
Charlotte H. Møller ◽  
Michael Etzerodt ◽  
Hans C. Thøgersen ◽  
Thor L. Holtet
Keyword(s):  
Directed Evolution ◽  
Blood Coagulation ◽  
Factor Xa ◽  
Coagulation Factor ◽  
Inhibitor Binding ◽  
Selection Parameters

Reaction pathway for inhibition of blood coagulation factor Xa by tick anticoagulant peptide

Biochemistry ◽  
1992 ◽  
Vol 31 (23) ◽  
pp. 5374-5380 ◽  
Author(s):  
Susan P. Jordan ◽  
Shi Shan Mao ◽  
Sidney D. Lewis ◽  
Jules A. Shafer
Keyword(s):  
Blood Coagulation ◽  
Reaction Pathway ◽  
Factor Xa ◽  
Coagulation Factor ◽  
Tick Anticoagulant Peptide

Two parallel prothrombin activator systems in Australian rough-scaled snake, Tropidechis carinatus

2005 ◽  
Vol 93 (01) ◽  
pp. 40-47 ◽  
Author(s):  
Md. Abu Reza ◽  
Sanjay Swarup ◽  
Manjunatha Kini
Keyword(s):  
Blood Coagulation ◽  
Blood Clotting ◽  
Cdna Sequence ◽  
Factor Xa ◽  
Coagulation Factor ◽  
Venom Gland ◽  
Factor X ◽  
Specific Expression ◽  
Life Saving ◽  
Sequence Encoding

SummaryIt is uncommon for similar pathways/systems to be involved in highly divergent functions within single organisms. Earlier, we have shown that trocarin D, a venom prothrombin activator, from the Australian rough-scaled snake Tropidechis carinatus, is structurally and functionally similar to the blood coagulation factor Xa (FXa). The presence of a haemostatic system in these snakes implies that they have two parallel prothrombin activating systems: one in the plasma, that participates in the life saving process of blood clotting and the other in their venom, where it acts as a toxin. Here, we report the complete cDNA sequence encoding the blood coagulation factor X (FX) from the liver of T. carinatus. Deduced T. carinatus FX sequence shows ~80% identity with trocarin D but ~50% identity with the mammalian FX. Our present study confirms the presence of two separate genes – one each for FX and trocarin D, that code for similar proteins in T. carinatus snake. These two genes have different expression sites and divergent uses suggesting that snake venom prothrombin activators have probably evolved by the duplication of the liver FX gene and subsequently marked for tissue-specific expression in the venom gland.

ChemInform Abstract: Dibasic (Amidinoaryl)propanoic Acid Derivatives as Novel Blood Coagulation Factor Xa Inhibitors.

ChemInform ◽  
2010 ◽  
Vol 25 (33) ◽  
pp. no-no
Author(s):  
T. NAGAHARA ◽  
Y. YOKOYAMA ◽  
K. INAMURA ◽  
S. KATAKURA ◽  
S. KOMORIYA ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Factor Xa ◽  
Coagulation Factor ◽  
Factor Xa Inhibitors ◽  
Propanoic Acid

Chlorothiophenecarboxamides as P1 surrogates of inhibitors of blood coagulation factor Xa

2004 ◽  
Vol 14 (23) ◽  
pp. 5817-5822 ◽  
Author(s):  
Werner W.K.R. Mederski ◽  
Bertram Cezanne ◽  
Christoph van Amsterdam ◽  
Karl-Ulrich Bühring ◽  
Dieter Dorsch ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Factor Xa ◽  
Coagulation Factor

scholarly journals Role of the blood coagulation cascade in hepatic fibrosis

2018 ◽  
Vol 315 (2) ◽  
pp. G171-G176 ◽  
Author(s):  
Asmita Pant ◽  
Anna K. Kopec ◽  
James P. Luyendyk
Keyword(s):  
Blood Coagulation ◽  
Hepatic Fibrosis ◽  
Animal Studies ◽  
Factor Xa ◽  
Coagulation Factor ◽  
Primary Source ◽  
Bleeding Risk ◽  
Normal Function ◽  
Coagulation Cascade ◽  
Rapid Progression

Liver is the primary source of numerous proteins that are critical for normal function of the blood coagulation cascade. Because of this, diseases of the liver, particularly when affiliated with severe complications like cirrhosis, are associated with abnormalities of blood clotting. Although conventional interpretation has inferred cirrhosis as a disorder of uniform bleeding risk, it is now increasingly appreciated as a disease wherein the coagulation cascade is precariously rebalanced. Moreover, prothrombotic risk factors are also associated with a more rapid progression of fibrosis in humans, suggesting that coagulation proteases participate in disease pathogenesis. Indeed, strong evidence drawn from experimental animal studies indicates that components of the coagulation cascade, particularly coagulation factor Xa and thrombin, drive profibrogenic events, leading to hepatic fibrosis. Here, we concisely review the evidence supporting a pathologic role for coagulation in the development of liver fibrosis and the potential mechanisms involved. Further, we highlight how studies in experimental animals may shed light on emerging clinical evidence, suggesting that beneficial effects of anticoagulation could extend beyond preventing thrombotic complications to include reducing pathologies like fibrosis.

ChemInform Abstract: (Z,Z)-2,7-Bis(4-amidinobenzylidene)cycloheptan-1-one: Identification of a Highly Active Inhibitor of Blood Coagulation Factor Xa.

ChemInform ◽  
2010 ◽  
Vol 30 (1) ◽  
pp. no-no
Author(s):  
K. J. SHAW ◽  
W. J. GUILFORD ◽  
J. L. DALLAS ◽  
S. K. KOOVAKKAAT ◽  
M. A. MCCARRICK ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Factor Xa ◽  
Coagulation Factor ◽  
Active Inhibitor ◽  
Highly Active
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