A ceramic electrochemical microreactor for the methoxylation of methyl-2-furoate with direct mass spectrometry coupling

Lab on a Chip ◽  
2002 ◽  
Vol 2 (1) ◽  
pp. 39 ◽  
Author(s):  
Virginie Mengeaud ◽  
Olivier Bagel ◽  
Rosaria Ferrigno ◽  
Hubert H. Girault ◽  
Akhtar Haider
Keyword(s):  
Mass Spectrometry ◽  
Direct Mass Spectrometry ◽  
Electrochemical Microreactor

Dicarbonyl derived post-translational modifications: chemistry bridging biology and aging-related disease

2020 ◽  
Vol 64 (1) ◽  
pp. 97-110
Author(s):  
Christian Sibbersen ◽  
Mogens Johannsen
Keyword(s):  
Mass Spectrometry ◽  
Future Research ◽  
Amino Acid Residues ◽  
Post Translational Modification ◽  
Dicarbonyl Compounds ◽  
Protein Targets ◽  
Post Translational Modifications ◽  
Reactive Protein ◽  
Glycation End Products ◽  
Direct Mass Spectrometry

Abstract In living systems, nucleophilic amino acid residues are prone to non-enzymatic post-translational modification by electrophiles. α-Dicarbonyl compounds are a special type of electrophiles that can react irreversibly with lysine, arginine, and cysteine residues via complex mechanisms to form post-translational modifications known as advanced glycation end-products (AGEs). Glyoxal, methylglyoxal, and 3-deoxyglucosone are the major endogenous dicarbonyls, with methylglyoxal being the most well-studied. There are several routes that lead to the formation of dicarbonyl compounds, most originating from glucose and glucose metabolism, such as the non-enzymatic decomposition of glycolytic intermediates and fructosyl amines. Although dicarbonyls are removed continuously mainly via the glyoxalase system, several conditions lead to an increase in dicarbonyl concentration and thereby AGE formation. AGEs have been implicated in diabetes and aging-related diseases, and for this reason the elucidation of their structure as well as protein targets is of great interest. Though the dicarbonyls and reactive protein side chains are of relatively simple nature, the structures of the adducts as well as their mechanism of formation are not that trivial. Furthermore, detection of sites of modification can be demanding and current best practices rely on either direct mass spectrometry or various methods of enrichment based on antibodies or click chemistry followed by mass spectrometry. Future research into the structure of these adducts and protein targets of dicarbonyl compounds may improve the understanding of how the mechanisms of diabetes and aging-related physiological damage occur.

scholarly journals Mechanistic Insights into Alzheimer’s Disease Unveiled through the Investigation of Disturbances in Central Metabolites and Metabolic Pathways

Biomedicines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 298
Author(s):  
Raúl González-Domínguez ◽  
Álvaro González-Domínguez ◽  
Ana Sayago ◽  
Juan Diego González-Sanz ◽  
Alfonso María Lechuga-Sancho ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Metabolic Pathways ◽  
Gas Chromatography Mass Spectrometry ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Multiple Primary ◽  
Human Disorders ◽  
Direct Mass Spectrometry

Hydrophilic metabolites are closely involved in multiple primary metabolic pathways and, consequently, play an essential role in the onset and progression of multifactorial human disorders, such as Alzheimer’s disease. This review article provides a comprehensive revision of the literature published on the use of mass spectrometry-based metabolomics platforms for approaching the central metabolome in Alzheimer’s disease research, including direct mass spectrometry, gas chromatography-mass spectrometry, hydrophilic interaction liquid chromatography-mass spectrometry, and capillary electrophoresis-mass spectrometry. Overall, mounting evidence points to profound disturbances that affect a multitude of central metabolic pathways, such as the energy-related metabolism, the urea cycle, the homeostasis of amino acids, fatty acids and nucleotides, neurotransmission, and others.

Direct Mass Spectrometry Differentiation of Ectopic and Eutopic Endometrium in Patients with Endometriosis

2018 ◽  
Vol 25 (3) ◽  
pp. 426-433 ◽  
Author(s):  
Leila V. Adamyan ◽  
Natalia Starodubtseva ◽  
Anna Borisova ◽  
Assia A. Stepanian ◽  
Vitaliy Chagovets ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Eutopic Endometrium ◽  
Direct Mass Spectrometry

Drug metabolite heterogeneity in cultured single cells profiled by pico-trapping direct mass spectrometry

Nanomedicine ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. 1365-1374 ◽  
Author(s):  
Yasufumi Fukano ◽  
Naohiro Tsuyama ◽  
Hajime Mizuno ◽  
Sachiko Date ◽  
Mikihisa Takano ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cells ◽  
Drug Metabolite ◽  
Direct Mass Spectrometry
Sign in / Sign up

Export Citation Format

Share Document