Single-molecule detection using confocal fluorescence detection: Assessment of optical probe volumes

The Analyst ◽  
2000 ◽  
Vol 125 (6) ◽  
pp. 1033-1036 ◽  
Author(s):  
Elisabeth K. Hill ◽  
Andrew J. de Mello
Keyword(s):  
Fluorescence Detection ◽  
Single Molecule ◽  
Optical Probe ◽  
Single Molecule Detection ◽  
Confocal Fluorescence

Single-Molecule Detection and Identification of Multiple Species by Multiparameter Fluorescence Detection

2006 ◽  
Vol 78 (6) ◽  
pp. 2039-2050 ◽  
Author(s):  
Jerker Widengren ◽  
Volodymyr Kudryavtsev ◽  
Matthew Antonik ◽  
Sylvia Berger ◽  
Margarita Gerken ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Detection And Identification ◽  
Multiple Species

scholarly journals A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases

2021 ◽  
Author(s):  
Li-juan Wang ◽  
Le Liang ◽  
Bing-jie Liu ◽  
BingHua Jiang ◽  
Chun-yang Zhang
Keyword(s):  
Single Molecule ◽  
Single Molecule Detection ◽  
Amplification Cascade

A controlled T7 transcription-driven symmetric amplification cascade machinery is developed for single-molecule detection of multiple repair glycosylases.

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

scholarly journals Nanopore Technology for the Application of Protein Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1942
Author(s):  
Xiaoqing Zeng ◽  
Yang Xiang ◽  
Qianshan Liu ◽  
Liang Wang ◽  
Qianyun Ma ◽  
...  
Keyword(s):  
Single Molecule ◽  
Protein Detection ◽  
High Sensitivity ◽  
Single Molecule Detection ◽  
Sequence Detection ◽  
Fast Detection ◽  
Material Basis ◽  
Sensing Technology ◽  
Detection Technology ◽  
Structure Recognition

Protein is an important component of all the cells and tissues of the human body and is the material basis of life. Its content, sequence, and spatial structure have a great impact on proteomics and human biology. It can reflect the important information of normal or pathophysiological processes and promote the development of new diagnoses and treatment methods. However, the current techniques of proteomics for protein analysis are limited by chemical modifications, large sample sizes, or cumbersome operations. Solving this problem requires overcoming huge challenges. Nanopore single molecule detection technology overcomes this shortcoming. As a new sensing technology, it has the advantages of no labeling, high sensitivity, fast detection speed, real-time monitoring, and simple operation. It is widely used in gene sequencing, detection of peptides and proteins, markers and microorganisms, and other biomolecules and metal ions. Therefore, based on the advantages of novel nanopore single-molecule detection technology, its application to protein sequence detection and structure recognition has also been proposed and developed. In this paper, the application of nanopore single-molecule detection technology in protein detection in recent years is reviewed, and its development prospect is investigated.

Single-Molecule Detection by Laser-Induced Fluorescence Technique with a Position-Sensitive Photon-Counting Apparatus

1994 ◽  
Vol 33 (Part 1, No. 3A) ◽  
pp. 1571-1576 ◽  
Author(s):  
Mitsuru Ishikawa ◽  
Ken-ichi Hirano ◽  
Tsuyoshi Hayakawa ◽  
Shigeru Hosoi ◽  
Sydney Brenner
Keyword(s):  
Single Molecule ◽  
Photon Counting ◽  
Laser Induced Fluorescence ◽  
Single Molecule Detection ◽  
Fluorescence Technique ◽  
Position Sensitive
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