Single-Molecule Detection and Identification of Multiple Species by Multiparameter Fluorescence Detection

2006 ◽  
Vol 78 (6) ◽  
pp. 2039-2050 ◽  
Author(s):  
Jerker Widengren ◽  
Volodymyr Kudryavtsev ◽  
Matthew Antonik ◽  
Sylvia Berger ◽  
Margarita Gerken ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Detection And Identification ◽  
Multiple Species

Cucurbituril mediated single molecule detection and identification via recognition tunneling

2018 ◽  
Vol 29 (36) ◽  
pp. 365501 ◽  
Author(s):  
Bohuai Xiao ◽  
Feng Liang ◽  
Simin Liu ◽  
JongOne Im ◽  
Yunchuan Li ◽  
...  
Keyword(s):  
Single Molecule ◽  
Single Molecule Detection ◽  
Detection And Identification ◽  
Recognition Tunneling

scholarly journals Single molecule fingerprinting reveals different amplification properties of α-synuclein oligomers and preformed fibrils in seeding assay.

2021 ◽  
Author(s):  
Derrick Lau ◽  
Chloe Magnan ◽  
Kathryn Hill ◽  
Antony Cooper ◽  
Yann Gambin ◽  
...  
Keyword(s):  
Single Molecule ◽  
Amyloid Fibrils ◽  
Size Exclusion ◽  
Single Molecule Detection ◽  
Single Molecule Spectroscopy ◽  
Parkinsons Disease ◽  
Exclusion Chromatography ◽  
Particle Level ◽  
Amplification Assay ◽  
Multiple Species

The quantification of α-synuclein (α-syn) aggregates has emerged as a promising biomarker for synucleinopathies. Assays that amplify and detect such aggregates have revealed the presence of seeding-competent species in biosamples of patients diagnosed with Parkinsons disease. However, multiple species such as oligomers and amyloid fibrils, are formed during the aggregation of α-synuclein and these species are likely to co-exist in biological samples and thus it remains unclear which species(s) are contributing to the signal detected in seeding assays. To identify which species can be detected in seeding assays, recombinant oligomers and preformed fibrils were produced and purified to characterise their individual biochemical and seeding potential. Here, we used single molecule spectroscopy to track the formation and purification of oligomers and fibrils at the single particle level and compare their respective seeding potential in an amplification assay. Single molecule detection validates that size-exclusion chromatography efficiently separates oligomers from fibrils. Oligomers were found to be seeding-competent but our results reveal that their seeding behaviour is very different compared to preformed fibrils in our amplification assay. Overall, our data suggest that even a low number of preformed fibrils present in biosamples are likely to dominate the response in seeding assays.

scholarly journals A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases

2021 ◽  
Author(s):  
Li-juan Wang ◽  
Le Liang ◽  
Bing-jie Liu ◽  
BingHua Jiang ◽  
Chun-yang Zhang
Keyword(s):  
Single Molecule ◽  
Single Molecule Detection ◽  
Amplification Cascade

A controlled T7 transcription-driven symmetric amplification cascade machinery is developed for single-molecule detection of multiple repair glycosylases.

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

scholarly journals Nanopore Technology for the Application of Protein Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1942
Author(s):  
Xiaoqing Zeng ◽  
Yang Xiang ◽  
Qianshan Liu ◽  
Liang Wang ◽  
Qianyun Ma ◽  
...  
Keyword(s):  
Single Molecule ◽  
Protein Detection ◽  
High Sensitivity ◽  
Single Molecule Detection ◽  
Sequence Detection ◽  
Fast Detection ◽  
Material Basis ◽  
Sensing Technology ◽  
Detection Technology ◽  
Structure Recognition

Protein is an important component of all the cells and tissues of the human body and is the material basis of life. Its content, sequence, and spatial structure have a great impact on proteomics and human biology. It can reflect the important information of normal or pathophysiological processes and promote the development of new diagnoses and treatment methods. However, the current techniques of proteomics for protein analysis are limited by chemical modifications, large sample sizes, or cumbersome operations. Solving this problem requires overcoming huge challenges. Nanopore single molecule detection technology overcomes this shortcoming. As a new sensing technology, it has the advantages of no labeling, high sensitivity, fast detection speed, real-time monitoring, and simple operation. It is widely used in gene sequencing, detection of peptides and proteins, markers and microorganisms, and other biomolecules and metal ions. Therefore, based on the advantages of novel nanopore single-molecule detection technology, its application to protein sequence detection and structure recognition has also been proposed and developed. In this paper, the application of nanopore single-molecule detection technology in protein detection in recent years is reviewed, and its development prospect is investigated.

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