Observations on the use of 2,4,6-trinitrobenzenesulphonic acid for the determination of available lysine in animal protein concentrates

The Analyst ◽  
1973 ◽  
Vol 98 (1170) ◽  
pp. 673 ◽  
Author(s):  
R. J. Hall ◽  
N. Trinder ◽  
D. I. Givens
Keyword(s):  
Animal Protein ◽  
Protein Concentrates ◽  
Available Lysine

scholarly journals Determination of protein and reactive lysine in leaf-protein concentrates by dye-binding

1979 ◽  
Vol 42 (3) ◽  
pp. 445-454 ◽  
Author(s):  
Ann F. Walker
Keyword(s):  
Convenient Method ◽  
Nutritional Value ◽  
Tungstic Acid ◽  
Leaf Protein ◽  
Processing Conditions ◽  
Acid Orange ◽  
Protein Concentrates ◽  
Dye Binding ◽  
Available Lysine

1. Twenty leaf-protein concentrates (LPC), were produced from different crops and by different processes, the latter being designed to retain maximum nutritional value of the samples.2. The establishment of conditions for the use of CI Acid Orange 12 in a commercial dye-buffer reagent for the determination of protein and reactive (available) lysine in LPC was investigated.3. Values for protein by dye-binding correlated well with those for tungstic-acid-precipitated nitrogen (×6.25).4. Some LPC samples showed a loss of reactive lysine, the greatest loss being associated with the most severe processing conditions.5. For the LPC samples studied, dye-binding provided a convenient method for the concurrent determination of protein and reactive lysine.

Liquid Chromatographic Determ [nation of Available Lysine in Soybean and Fish Meal

1988 ◽  
Vol 71 (2) ◽  
pp. 350-353
Author(s):  
Joan Rabasseda ◽  
Gemma Rauret ◽  
Teresa M Galceran
Keyword(s):  
Liquid Chromatography ◽  
Simplex Method ◽  
Fish Meal ◽  
Amino Group ◽  
Optimum Conditions ◽  
Available Lysine ◽  
Liquid Chromatographic

Abstract A method for the determination of available lysine on the basis of the reactivity of the e-amino group with fluorodinitrobenzene (FDNB) has been optimized. Hydrolysis is performed in closed vessels in an autoclave. Conditions for different meals were established by using a modified sequential simplex method. Hydrolysis for 4 h was sufficient for the meals studied—soybean and fish. The use of liquid chromatography to determine available lysine was studied, and optimum conditions were established for separation and quantitation of e-DNB-lysine. The proposed method is faster, more accurate, and more precise than commonly used methods

Quantitative Determination of Coumestrol in Dried Alfalfa and Alfalfa Leaf Protein Concentrates Containing Chlorophyll

1975 ◽  
Vol 58 (5) ◽  
pp. 983-986
Author(s):  
Benny E Knuckles ◽  
Raymond E Miller ◽  
E M Bickoff
Keyword(s):  
Quantitative Determination ◽  
Analytical Method ◽  
Leaf Protein ◽  
Improved Method ◽  
Alcohol Extract ◽  
Protein Concentrates ◽  
Coefficients Of Variation ◽  
Lower Standard ◽  
Alfalfa Leaf

Abstract An improved analytical method for the determination of coumestrol in dried alfalfa and leaf protein concentrates is described. In this method, chlorophyll is removed from an alcohol extract prior to the paper chromatographic-fluorometric measurement of coumestrol. Ninety-eight per cent of the coumestrol added to alfalfa leaf protein concentrates is recovered by this method. This improved method gives replicate values with lower standard deviations and coefficients of variation than the literature method.

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