The application of gas chromatography to the examination of the constituents of Cannabis sativa L.

The Analyst ◽  
1967 ◽  
Vol 92 (1096) ◽  
pp. 450 ◽  
Author(s):  
L. T. Heaysman ◽  
E. A. Walker ◽  
D. T. Lewis
Keyword(s):  
Gas Chromatography ◽  
Cannabis Sativa

scholarly journals Exploring the Mysteries of Cannabis through Gas Chromatography

2020 ◽  
Author(s):  
María Teresa García-Valverde ◽  
Verónica Sánchez de Medina ◽  
Verónica Codesido ◽  
Jesús Hidalgo-García ◽  
Carlos Ferreiro-Vera
Keyword(s):  
Gas Chromatography ◽  
Quality Assurance ◽  
Product Quality ◽  
Health Effects ◽  
Cannabis Sativa ◽  
Sample Complexity ◽  
Active Constituents ◽  
Strong Interest ◽  
Recent Advances ◽  
Good Manufacturing Practices

In the last decades, cannabinoids, the active constituents of Cannabis sativa L., have been attracting a strong interest, regarding the health effects associated with the use of Cannabis and Cannabis-derived products. The progressive legalization of this species in several countries has prompted an increasing concern about the characterization and quantification of cannabinoids in diverse chemotypes of the plant, as well as the obtained final products. Therewith, Process and Product Quality Assurance (PPQA) becomes a mandatory practise to verify the Good Manufacturing Practices (GMP). Gas chromatography is one of the most used techniques in this sense due to its high attainable resolution. However, sample complexity and the thermal lability of cannabinoids hinder the analysis. In this chapter, a fully description of the recent advances in the Cannabis sativa L. analysis by gas chromatography will be presented, including different approaches that have come up to solve the obstacles encountered.

Gas chromatography of Indian hemp (Cannabis sativa L.)

1967 ◽  
Vol 19 (12) ◽  
pp. 851-852 ◽  
Author(s):  
B. Caddy ◽  
F. Fish ◽  
W. D. C. Wilson
Keyword(s):  
Gas Chromatography ◽  
Cannabis Sativa ◽  
Indian Hemp

scholarly journals Terpenes/Terpenoids in Cannabis: Are They Important?

2020 ◽  
Vol 3 (1) ◽  
pp. 25-60
Author(s):  
Lumír Ondřej Hanuš ◽  
Yotam Hod
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Medical Treatment ◽  
Essential Oils ◽  
Cannabis Sativa ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Plant Materials ◽  
Chromatography Mass Spectrometry ◽  
Mass Spectrometry Detector

Cannabis sativa plant has not only cannabinoids as crucial compounds but also the other compounds that play important role as synergistic and/or entourage compound. Cannabis/hemp plant materials and essential oils were analyzed with the help of gas chromatography/mass spectrometry detector for the content of terpenes and terpenoids. The main terpenes/terpenoids and their abundance in the samples were evaluated. Results of this study will be helpful in the next evaluation of these compound in mixture with cannabinoids and their importance in medical treatment.

scholarly journals The Inheritance of Chemical Phenotype in Cannabis sativa L.

Genetics ◽  
2003 ◽  
Vol 163 (1) ◽  
pp. 335-346 ◽  
Author(s):  
Etienne P M de Meijer ◽  
Manuela Bagatta ◽  
Andrea Carboni ◽  
Paola Crucitti ◽  
V M Cristiana Moliterni ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Bulk Segregant Analysis ◽  
Cannabis Sativa ◽  
Scar Marker ◽  
Sequence Characterized Amplified Region ◽  
B Locus ◽  
Common Precursor ◽  
Tight Linkage ◽  
The Common

Abstract Four crosses were made between inbred Cannabis sativa plants with pure cannabidiol (CBD) and pure Δ-9-tetrahydrocannabinol (THC) chemotypes. All the plants belonging to the F1’s were analyzed by gas chromatography for cannabinoid composition and constantly found to have a mixed CBD-THC chemotype. Ten individual F1 plants were self-fertilized, and 10 inbred F2 offspring were collected and analyzed. In all cases, a segregation of the three chemotypes (pure CBD, mixed CBD-THC, and pure THC) fitting a 1:2:1 proportion was observed. The CBD/THC ratio was found to be significantly progeny specific and transmitted from each F1 to the F2’s derived from it. A model involving one locus, B, with two alleles, BD and BT, is proposed, with the two alleles being codominant. The mixed chemotypes are interpreted as due to the genotype BD/BT at the B locus, while the pure-chemotype plants are due to homozygosity at the B locus (either BD/BD or BT/BT). It is suggested that such codominance is due to the codification by the two alleles for different isoforms of the same synthase, having different specificity for the conversion of the common precursor cannabigerol into CBD or THC, respectively. The F2 segregating groups were used in a bulk segregant analysis of the pooled DNAs for screening RAPD primers; three chemotype-associated markers are described, one of which has been transformed in a sequence-characterized amplified region (SCAR) marker and shows tight linkage to the chemotype and codominance.

scholarly journals Quantitative analysis of Δ9-THC-COOH in Human Urine by the Liquid-Liquid Extraction technique and Gas Chromatography-Mass Spectrometry: Adaptation, Optimization and Validation

2021 ◽  
Vol 8 ◽  
Author(s):  
Kleber Oliveira ◽  
Deborah Scanferla ◽  
Jordana Silva ◽  
Mariana Madia ◽  
Erika Bando ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Drugs Of Abuse ◽  
Cannabis Sativa ◽  
Low Cost ◽  
Liquid Extraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Validation Process ◽  
Liquid Liquid Extraction

The main active compound of Cannabis sativa is Δ9-tetrahydrocannabinol, which is quickly transformed into 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (Δ9-THC-COOH) in the human body. This research aimed to validate an efficient, fast and low-cost technique for Δ9-THC-COOH analysis in urine with adaptations of existing analytical methods. The validation process was carried out in accordance with guidelines published by ANVISA and with international guidelines. The analyte was extracted by liquid-liquid extraction and identified/quantified by gas chromatography coupled to mass spectrometry. Linear curves ranges were from 5 to 300 ng mL-1 (r = 0.9993; y= 0.0269x – 0.0364). Intra and inter-day precision varied from 3.38 to 9.04% and accuracy was between 83 to 112.9%. The Δ9-THC-COOH remained stable after 15-30 days of storage at -20 °C (long-term test), after 5 freeze-thaw cycles and post-processing for up to 72 hours. The method is fast, low-cost, with detection limits and quantification below the cut-off (15 ng mL-1), which makes it useful and efficient for routine use at toxicology laboratories, for drug addiction and doping control, for forensic purposes and also for controlling the use of drugs of abuse by vehicle drivers.

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