Dithioamide metal ion receptors on fluorescent lipid bilayers for the selective optical detection of mercuric ion

1998 ◽  
pp. 1581-1582 ◽  
Author(s):  
Darryl Y. Sasaki ◽  
Benjamin E. Padilla
Keyword(s):  
Lipid Bilayers ◽  
Metal Ion ◽  
Optical Detection ◽  
Mercuric Ion ◽  
Ion Receptors ◽  
Fluorescent Lipid

Genetically Engineered Pores as Metal Ion Biosensors

1993 ◽  
Vol 330 ◽  
Author(s):  
John Kasianowicz ◽  
Barbara Walker ◽  
Musti Krishnasastry ◽  
Hagan Bayley
Keyword(s):  
Response Time ◽  
Lipid Bilayers ◽  
Metal Ion ◽  
Dynamic Range ◽  
Divalent Cations ◽  
High Sensitivity ◽  
Genetically Engineered ◽  
Great Promise ◽  
Lipid Bilayer Membranes ◽  
Time Dynamic

ABSTRACTWe are adapting proteins that form pores in lipid bilayers for use as components of biosensors. Specifically, we have produced genetically engineered variants of the α hemolysin (αHL) fromStaphylococcusaureus with properties that are sensitive to low concentrations of divalent cations. For example, the pore-forming activity of one mutant (αHL-H5: residues 130–134 inclusive replaced with histidine) is inhibited by Zn2+at concentrations as low as 1 μM, as judged by the reduction in its ability to lyse rabbit red blood cells and to increase the conductance of planar lipid bilayer membranes. When αHL-H5 is added to the aqueous phase bathing one side of a planar membrane, the subsequent addition of 100 μM Zn2+to either side blocks the pores that form. This result suggests that at least part of the mutated region lines the channel lumen. Ca2+and Mg2+do not block the channel and therefore the H5 mutation confers a degree of analyte specificity to the αHL pore. The results suggest that genetically engineered pores have great promise for the rapid and sensitive detection of metal cations and we discuss the merits and potential limitations for their use in this application. Specifically, we examine the issues of selectivity, sensitivity, response time, dynamic range and longevity. Some of these properties are interdependent. For example, the goals of high sensitivity and rapid response time can be in conflict.

scholarly journals Thin-film silica sol-gels doped with ion-responsive fluorescent lipid bilayers

1999 ◽  
Author(s):  
Darryl Y. Sasaki ◽  
Lauren E. Shea ◽  
Michael B. Sinclair
Keyword(s):  
Thin Film ◽  
Lipid Bilayers ◽  
Silica Sol ◽  
Fluorescent Lipid

ChemInform Abstract: Synthesis of Novel Azamacrocyclic Metal Ion Receptors Using a Modified Mannich Aminomethylation Reaction

ChemInform ◽  
2010 ◽  
Vol 30 (24) ◽  
pp. no-no
Author(s):  
Jerald S. Bradshaw ◽  
Andrei V. Bordunov ◽  
Xian Xin Zhang ◽  
Victor N. Pastushok ◽  
Reed M. Izatt
Keyword(s):  
Metal Ion ◽  
Aminomethylation Reaction ◽  
Mannich Aminomethylation ◽  
Ion Receptors

Tools and Tactics for the Optical Detection of Mercuric Ion

2008 ◽  
Vol 108 (9) ◽  
pp. 3443-3480 ◽  
Author(s):  
Elizabeth M. Nolan ◽  
Stephen J. Lippard
Keyword(s):  
Optical Detection ◽  
Mercuric Ion

Diaza-18-Crown-6 Ligands Containing Two Aminophenol Side Arms:  New Heterobinuclear Metal Ion Receptors

1999 ◽  
Vol 64 (11) ◽  
pp. 3825-3829 ◽  
Author(s):  
Ning Su ◽  
Jerald S. Bradshaw ◽  
Xian Xin Zhang ◽  
Paul B. Savage ◽  
Krzysztof E. Krakowiak ◽  
...  
Keyword(s):  
Metal Ion ◽  
Ion Receptors

Azacrown ethers containing oximic and Schiff base sidearms - potential heteronuclear metal ion receptors

Tetrahedron ◽  
1997 ◽  
Vol 53 (52) ◽  
pp. 17595-17606 ◽  
Author(s):  
Andrei V. Bordunov ◽  
Jerald S. Bradshaw ◽  
Victor N. Pastushok ◽  
Xian X. Zhang ◽  
Xiaolan Kou ◽  
...  
Keyword(s):  
Schiff Base ◽  
Metal Ion ◽  
Azacrown Ethers ◽  
Ion Receptors

Diaza- 18-Crown-6 Ligands Containing Two Aminophenol Side Arms: New Heterobinuclear Metal Ion Receptors.

1999 ◽  
Author(s):  
Ning Su ◽  
Jerald S. Bradshaw ◽  
Xian X. Zhang ◽  
Paul B. Savage ◽  
Krzysztof E. Krakowiak
Keyword(s):  
Metal Ion ◽  
Ion Receptors

ChemInform Abstract: Diaza-18-Crown-6 Ligands Containing Two Aminophenol Side Arms: New Heterobinuclear Metal Ion Receptors.

ChemInform ◽  
2010 ◽  
Vol 30 (34) ◽  
pp. no-no
Author(s):  
Ning Su ◽  
Jerald S. Bradshaw ◽  
Xian Xin Zhang ◽  
Paul B. Savage ◽  
Krzysztof E. Krakowiak ◽  
...  
Keyword(s):  
Metal Ion ◽  
Ion Receptors

scholarly journals Metal ion quenching of perylene fluorescence in lipid bilayers

1993 ◽  
Vol 65 (8) ◽  
pp. 1687-1692 ◽  
Author(s):  
D. J. S. Birch ◽  
K. Suhling ◽  
A. S. Holmes ◽  
T. Salthammer ◽  
R. E. Imhof
Keyword(s):  
Lipid Bilayers ◽  
Metal Ion

A polyelectrolyte bearing metal ion receptors and electrostatic functionality for layer-by-layer self-assembly

2004 ◽  
Vol 210 (1) ◽  
pp. 311-319 ◽  
Author(s):  
Henning Krass ◽  
Georg Papastavrou ◽  
Dirk G. Kurth
Keyword(s):  
Self Assembly ◽  
Metal Ion ◽  
Layer By Layer ◽  
Ion Receptors
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