scholarly journals Dynamic monitoring of membrane nanotubes formation induced by vaccinia virus on a high throughput microfluidic chip

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Min Xiao ◽  
Na Xu ◽  
Cheng Wang ◽  
Dai-Wen Pang ◽  
Zhi-Ling Zhang
Keyword(s):  
Vaccinia Virus ◽  
High Throughput ◽  
Microfluidic Chip ◽  
Dynamic Monitoring ◽  
Membrane Nanotubes

scholarly journals Neutralization Assay Using a Modified Vaccinia Virus Ankara Vector Expressing the Green Fluorescent Protein Is a High-Throughput Method To Monitor the Humoral Immune Response against Vaccinia Virus

2004 ◽  
Vol 11 (2) ◽  
pp. 406-410 ◽  
Author(s):  
Antonio Cosma ◽  
Silja Bühler ◽  
Rashmi Nagaraj ◽  
Caroline Staib ◽  
Anna-Lena Hammarin ◽  
...  
Keyword(s):  
Immune Response ◽  
Green Fluorescent Protein ◽  
Vaccinia Virus ◽  
High Throughput ◽  
Neutralizing Antibodies ◽  
Fluorescent Protein ◽  
Safety Concern ◽  
Neutralization Assay ◽  
Modified Vaccinia Virus Ankara ◽  
Green Fluorescent

ABSTRACT Vaccination against smallpox is again considered in order to face a possible bioterrorist threat, but the nature and the level of the immune response needed to protect a person from smallpox after vaccination are not totally understood. Therefore, simple, rapid, and accurate assays to evaluate the immune response to vaccinia virus need to be developed. Neutralization assays are usually considered good predictors of vaccine efficacy and more informative with regard to protection than binding assays. Currently, the presence of neutralizing antibodies to vaccinia virus is measured using a plaque reduction neutralization test, but this method is time-consuming and labor-intensive and has a subjective readout. Here, we describe an innovative neutralization assay based on a modified vaccinia virus Ankara (MVA) vector expressing the green fluorescent protein (MVA-gfp). This MVA-gfp neutralization assay is rapid and sensitive and has a high-throughput potential. Thus, it is suitable to monitor the immune response and eventually the efficacy of a large campaign of vaccination against smallpox and to study the vector-specific immune response in clinical trials that use genetically engineered vaccinia viruses. Most importantly, application of the highly attenuated MVA eliminates the safety concern in using the replication-competent vaccinia virus in the standard clinical laboratory.

Microfluidic Combinatorial Screening Platform

2012 ◽  
Author(s):  
Helena Zec ◽  
Tushar D. Rane ◽  
Wen-Chy Chu ◽  
Tza-Huei Wang
Keyword(s):  
High Throughput ◽  
Microfluidic Chip ◽  
Linear Array ◽  
Large Array ◽  
Snp Detection ◽  
Combinatorial Screening ◽  
Biological Assays ◽  
Reagent Consumption ◽  
Daughter Droplets ◽  
Screening Platform

We propose a microfluidic droplet-based platform that accepts an unlimited number of sample plugs from a multi-well plate, performs splitting of these sample droplets into smaller daughter droplets and subsequent synchronization-free, reliable fusion of sample daughter droplets with multiple reagents simultaneously. This system consists of two components: 1) a custom autosampler which generates a linear array of sub-microliter plugs in a microcapillary from a multi-well plate and 2) A microfluidic chip with channels for sample plug introduction, reagent merging and droplet incubation. This novel system generates large arrays of heterogeneous droplets from hundreds to thousands of samples while concurrently screening these arrays against a large array of reagents. This high throughput system minimizes sample and reagent consumption and can be applied to a gamut of biological assays, ranging from SNP detection to forensic screening.

Drug effects analysis on cells using a high throughput microfluidic chip

2010 ◽  
Vol 13 (1) ◽  
pp. 215-219 ◽  
Author(s):  
Zhongcheng Gong ◽  
Hong Zhao ◽  
Tianhua Zhang ◽  
Fang Nie ◽  
Pushparaj Pathak ◽  
...  
Keyword(s):  
High Throughput ◽  
Microfluidic Chip ◽  
Drug Effects

Expression Profiling of Herpesvirus and Vaccinia Virus Proteins Using a High-Throughput Baculovirus Screening System

2005 ◽  
Vol 4 (6) ◽  
pp. 2225-2235 ◽  
Author(s):  
Mian Gao ◽  
Nicole Brufatto ◽  
Tricia Chen ◽  
Laura Lea Murley ◽  
Rosanne Thalakada ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
High Throughput ◽  
Expression Profiling ◽  
Screening System ◽  
Virus Proteins

A high-throughput microfluidic chip for size sorting of cells

2011 ◽  
Author(s):  
Xuebin Tan ◽  
Hyeun-Joong Yoon ◽  
James Granneman ◽  
Hsiao-Ping Moore ◽  
Mark Ming-Cheng Cheng
Keyword(s):  
High Throughput ◽  
Microfluidic Chip ◽  
Size Sorting

Rational design of a turn-on fluorescent sensor for α-ketoglutaric acid in a microfluidic chip

2014 ◽  
Vol 5 (10) ◽  
pp. 4012-4016 ◽  
Author(s):  
Pengwei Jin ◽  
Changhong Jiao ◽  
Zhiqian Guo ◽  
Ye He ◽  
Shiqin Zhu ◽  
...  
Keyword(s):  
High Throughput ◽  
Microfluidic Chip ◽  
High Throughput Screening ◽  
Rational Design ◽  
Fluorescent Sensor ◽  
Fluorescent Chemosensors ◽  
Turn On

A rational design of turn-on fluorescent chemosensors for monitoring α-ketoglutaric acid has been developed with a microfluidic chip, indicative of a potential platform for high-throughput screening and monitoring of kinetics, especially in biological fields.

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