scholarly journals Labeling adipose derived stem cell sheet by ultrasmall super-paramagnetic Fe3O4 nanoparticles and magnetic resonance tracking in vivo

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Shukui Zhou ◽  
Ting Yin ◽  
Qingsong Zou ◽  
Kaile Zhang ◽  
Guo Gao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Magnetic Resonance ◽  
Fe3o4 Nanoparticles ◽  
Cell Sheet ◽  
Adipose Derived Stem Cell

In vivo construction of liver tissue by implantation of a hepatic non-parenchymal/adipose-derived stem cell sheet

2017 ◽  
Vol 12 (1) ◽  
pp. e287-e295 ◽  
Author(s):  
Mio Fujii ◽  
Kosho Yamanouchi ◽  
Yusuke Sakai ◽  
Zhassulan Baimakhanov ◽  
Izumi Yamaguchi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Liver Tissue ◽  
Cell Sheet ◽  
Adipose Derived Stem Cell

Monitoring stem cell migration in the nervous system by in vivo magnetic resonance imaging

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S692-S692
Author(s):  
Mathias Hoehn ◽  
Uwe Himmelreich ◽  
Ralph Weber ◽  
Pedro Ramos-Cabrer ◽  
Susanne Wegener ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Nervous System ◽  
Stem Cell ◽  
Cell Migration ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Stem Cell Migration

Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction

Biomaterials ◽  
2014 ◽  
Vol 35 (1) ◽  
pp. 174-184 ◽  
Author(s):  
Tsung-Szu Yeh ◽  
Yu-Hua Dean Fang ◽  
Chia-Hsin Lu ◽  
Shao-Chieh Chiu ◽  
Chia-Lin Yeh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Sheet ◽  
Myocardium Infarction ◽  
Adipose Derived Stem Cell

Elimination of Reperfusion-Induced Microcirculatory Alterations In Vivo by Adipose-Derived Stem Cell Supernatant without Adipose-Derived Stem Cells

2015 ◽  
Vol 135 (4) ◽  
pp. 1056-1064 ◽  
Author(s):  
Wei Z. Wang ◽  
Xin-Hua Fang ◽  
Shelley J. Williams ◽  
Linda L. Stephenson ◽  
Richard C. Baynosa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Adipose Derived Stem Cells ◽  
Adipose Derived Stem Cell ◽  
Cell Supernatant

scholarly journals Longitudinal Monitoring of Stem Cell Grafts In Vivo Using Magnetic Resonance Imaging with Inducible Maga as a Genetic Reporter

Theranostics ◽  
2014 ◽  
Vol 4 (10) ◽  
pp. 972-989 ◽  
Author(s):  
In K. Cho ◽  
Sean P. Moran ◽  
Ramesh Paudyal ◽  
Karolina Piotrowska-Nitsche ◽  
Pei-Hsun Cheng ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cell ◽  
Magnetic Resonance ◽  
Resonance Imaging

scholarly journals The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Shukui Zhou ◽  
Ying Wang ◽  
Kaile Zhang ◽  
Nailong Cao ◽  
Ranxing Yang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Regenerative Medicine ◽  
Silk Fibroin ◽  
Surgical Complication ◽  
Cell Sheet ◽  
The Body ◽  
Acellular Matrix ◽  
Triton X

To date, the decellularized scaffold has been widely explored as a source of biological scaffolds for regenerative medicine. However, the acellular matrix derived from natural tissues and organs has a lot of defects, including the limited amount of autogenous tissue and surgical complication such as risk of blood loss, wound infection, pain, shock, and functional damage in the donor part of the body. In this study, we prepared acellular matrix using adipose-derived stem cell (ADSC) sheets and evaluate the cellular compatibility and immunoreactivity. The ADSC sheets were fabricated and subsequently decellularized using repeated freeze-thaw, Triton X-100 and SDS decellularization. Oral mucosal epithelial cells were seeded onto the decellularized ADSC sheets to evaluate the cell replantation ability, and silk fibroin was used as the control. Then, acellular matrix was transplanted onto subcutaneous tissue for 1 week or 3 weeks; H&E staining and immunohistochemical analysis of CD68 expression and quantitative real-time PCR (qPCR) were performed to evaluate the immunogenicity and biocompatibility. The ADSC sheet-derived ECM scaffolds preserved the three-dimensional architecture of ECM and retained the cytokines by Triton X-100 decellularization protocols. Compared with silk fibroin in vitro, the oral mucosal epithelial cells survived better on the decellularized ADSC sheets with an intact and consecutive epidermal cellular layer. Compared with porcine small intestinal submucosa (SIS) in vivo, the homogeneous decellularized ADSC sheets had less monocyte-macrophage infiltrating in vivo implantation. During 3 weeks after transplantation, the mRNA expression of cytokines, such as IL-4/IL-10, was obviously higher in decellularized ADSC sheets than that of porcine SIS. A Triton X-100 method can achieve effective cell removal, retain major ECM components, and preserve the ultrastructure of ADSC sheets. The decellularized ADSC sheets possess good recellularization capacity and excellent biocompatibility. This study demonstrated the potential suitability of utilizing acellular matrix from ADSC sheets for soft tissue regeneration and repair.

scholarly journals Adipose-derived stem cell sheet transplantation therapy in a porcine model of chronic heart failure

2015 ◽  
Vol 165 (5) ◽  
pp. 631-639 ◽  
Author(s):  
Osamu Ishida ◽  
Ikuo Hagino ◽  
Noritoshi Nagaya ◽  
Tatsuya Shimizu ◽  
Teruo Okano ◽  
...  
Keyword(s):  
Heart Failure ◽  
Stem Cell ◽  
Chronic Heart Failure ◽  
Porcine Model ◽  
Cell Sheet ◽  
Adipose Derived Stem Cell ◽  
Transplantation Therapy

Multifunctional silica-coated iron oxide nanoparticles: a facile four-in-one system for in situ study of neural stem cell harvesting

2014 ◽  
Vol 175 ◽  
pp. 13-26 ◽  
Author(s):  
Yung-Kang Peng ◽  
Cathy N. P. Lui ◽  
Tsen-Hsuan Lin ◽  
Chen Chang ◽  
Pi-Tai Chou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Iron Oxide ◽  
Magnetic Resonance ◽  
Neural Stem Cells ◽  
Neural Stem Cell ◽  
Cell Therapies ◽  
Bimodal Imaging

Neural stem cells (NSCs), which generate the main phenotypes of the nervous system, are multipotent cells and are able to differentiate into multiple cell types via external stimuli from the environment. The extraction, modification and re-application of NSCs have thus attracted much attention and raised hopes for novel neural stem cell therapies and regenerative medicine. However, few studies have successfully identified the distribution of NSCs in a live brain and monitored the corresponding extraction processes both in vitro and in vivo. To address those difficulties, in this study multi-functional uniform nanoparticles comprising an iron oxide core and a functionalized silica shell (Fe3O4@SiO2(FITC)-CD133, FITC: a green emissive dye, CD133: anti-CD133 antibody) have been strategically designed and synthesized for use as probe nanocomposites that provide four-in-one functionality, i.e., magnetic agitation, dual imaging (both magnetic resonance and optical) and specific targeting. It is shown that these newly synthesized Fe3O4@SiO2(FITC)-CD133 particles have clearly demonstrated their versatility in various applications. (1) The magnetic core enables magnetic cell collection and T2 magnetic resonance imaging. (2) The fluorescent FITC embedded in the silica framework enables optical imaging. (3) CD133 anchored on the outermost surface is demonstrated to be capable of targeting neural stem cells for cell collection and bimodal imaging.

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