In vivo construction of liver tissue by implantation of a hepatic non-parenchymal/adipose-derived stem cell sheet

2017 ◽  
Vol 12 (1) ◽  
pp. e287-e295 ◽  
Author(s):  
Mio Fujii ◽  
Kosho Yamanouchi ◽  
Yusuke Sakai ◽  
Zhassulan Baimakhanov ◽  
Izumi Yamaguchi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Liver Tissue ◽  
Cell Sheet ◽  
Adipose Derived Stem Cell

Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction

Biomaterials ◽  
2014 ◽  
Vol 35 (1) ◽  
pp. 174-184 ◽  
Author(s):  
Tsung-Szu Yeh ◽  
Yu-Hua Dean Fang ◽  
Chia-Hsin Lu ◽  
Shao-Chieh Chiu ◽  
Chia-Lin Yeh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Sheet ◽  
Myocardium Infarction ◽  
Adipose Derived Stem Cell

Elimination of Reperfusion-Induced Microcirculatory Alterations In Vivo by Adipose-Derived Stem Cell Supernatant without Adipose-Derived Stem Cells

2015 ◽  
Vol 135 (4) ◽  
pp. 1056-1064 ◽  
Author(s):  
Wei Z. Wang ◽  
Xin-Hua Fang ◽  
Shelley J. Williams ◽  
Linda L. Stephenson ◽  
Richard C. Baynosa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Adipose Derived Stem Cells ◽  
Adipose Derived Stem Cell ◽  
Cell Supernatant

scholarly journals The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Shukui Zhou ◽  
Ying Wang ◽  
Kaile Zhang ◽  
Nailong Cao ◽  
Ranxing Yang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Regenerative Medicine ◽  
Silk Fibroin ◽  
Surgical Complication ◽  
Cell Sheet ◽  
The Body ◽  
Acellular Matrix ◽  
Triton X

To date, the decellularized scaffold has been widely explored as a source of biological scaffolds for regenerative medicine. However, the acellular matrix derived from natural tissues and organs has a lot of defects, including the limited amount of autogenous tissue and surgical complication such as risk of blood loss, wound infection, pain, shock, and functional damage in the donor part of the body. In this study, we prepared acellular matrix using adipose-derived stem cell (ADSC) sheets and evaluate the cellular compatibility and immunoreactivity. The ADSC sheets were fabricated and subsequently decellularized using repeated freeze-thaw, Triton X-100 and SDS decellularization. Oral mucosal epithelial cells were seeded onto the decellularized ADSC sheets to evaluate the cell replantation ability, and silk fibroin was used as the control. Then, acellular matrix was transplanted onto subcutaneous tissue for 1 week or 3 weeks; H&E staining and immunohistochemical analysis of CD68 expression and quantitative real-time PCR (qPCR) were performed to evaluate the immunogenicity and biocompatibility. The ADSC sheet-derived ECM scaffolds preserved the three-dimensional architecture of ECM and retained the cytokines by Triton X-100 decellularization protocols. Compared with silk fibroin in vitro, the oral mucosal epithelial cells survived better on the decellularized ADSC sheets with an intact and consecutive epidermal cellular layer. Compared with porcine small intestinal submucosa (SIS) in vivo, the homogeneous decellularized ADSC sheets had less monocyte-macrophage infiltrating in vivo implantation. During 3 weeks after transplantation, the mRNA expression of cytokines, such as IL-4/IL-10, was obviously higher in decellularized ADSC sheets than that of porcine SIS. A Triton X-100 method can achieve effective cell removal, retain major ECM components, and preserve the ultrastructure of ADSC sheets. The decellularized ADSC sheets possess good recellularization capacity and excellent biocompatibility. This study demonstrated the potential suitability of utilizing acellular matrix from ADSC sheets for soft tissue regeneration and repair.

scholarly journals Adipose-derived stem cell sheet transplantation therapy in a porcine model of chronic heart failure

2015 ◽  
Vol 165 (5) ◽  
pp. 631-639 ◽  
Author(s):  
Osamu Ishida ◽  
Ikuo Hagino ◽  
Noritoshi Nagaya ◽  
Tatsuya Shimizu ◽  
Teruo Okano ◽  
...  
Keyword(s):  
Heart Failure ◽  
Stem Cell ◽  
Chronic Heart Failure ◽  
Porcine Model ◽  
Cell Sheet ◽  
Adipose Derived Stem Cell ◽  
Transplantation Therapy

scholarly journals Local Application of Semaphorin 3A Combined with Adipose-Derived Stem Cell Sheet and Anorganic Bovine Bone Granules Enhances Bone Regeneration in Type 2 Diabetes Mellitus Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Xiaoru Xu ◽  
Kaixiu Fang ◽  
Lifeng Wang ◽  
Xiangwei Liu ◽  
Yuchao Zhou ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Stem Cell ◽  
Bone Healing ◽  
Cell Sheet ◽  
Semaphorin 3A ◽  
Adipose Derived Stem Cell

Bone tissue regeneration is considered to be the optimal solution for bone loss. However, diabetic patients have a greater risk of poor bone healing or bone grafting failure than nondiabetics. The purpose of this study was to investigate the influence of the complexes of an adipose-derived stem cell sheet (ASC sheet) and Bio-Oss® bone granules on bone healing in type 2 diabetes mellitus (T2DM) rats with the addition of semaphorin 3A (Sema3A). The rat ASC sheets showed stronger osteogenic ability than ASCs in vitro, as indicated by the extracellular matrix mineralization and the expression of osteogenesis-related genes at mRNA level. An ASC sheet combined with Bio-Oss® bone granules promoted bone formation in T2DM rats as indicated by microcomputed tomography (micro-CT) and histological analysis. In addition, Sema3A promoted the osteogenic differentiation of ASC sheets in vitro and local injection of Sema3A promoted T2DM rats’ calvarial bone regeneration based on ASC sheet and Bio-Oss® bone granule complex treatment. In conclusion, the local injection of Sema3A and the complexes of ASC sheet and Bio-Oss® bone granules could promote osseous healing and are potentially useful to improve bone healing for T2DM patients.

Transplantation of Adipose-Derived Stem Cell Sheet Attenuates Adverse Cardiac Remodeling in Acute Myocardial Infarction

2017 ◽  
Vol 23 (1-2) ◽  
pp. 1-11 ◽  
Author(s):  
Jong-Ho Kim ◽  
Hyung Joon Joo ◽  
Mina Kim ◽  
Seung-Cheol Choi ◽  
Jeong Ik Lee ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Stem Cell ◽  
Cardiac Remodeling ◽  
Cell Sheet ◽  
Adipose Derived Stem Cell

scholarly journals Differentiated Adipose-Derived Stem Cell Cocultures for Bone Regeneration in Polymer Scaffolds In Vivo

2014 ◽  
Vol 25 (4) ◽  
pp. 1504-1509 ◽  
Author(s):  
Amita R. Shah ◽  
Agustin Cornejo ◽  
Teja Guda ◽  
David E. Sahar ◽  
Stacy M. Stephenson ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Regeneration ◽  
Polymer Scaffolds ◽  
Adipose Derived Stem Cell
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