scholarly journals Mechanistic insights into the impact of Cold Atmospheric Pressure Plasma on human epithelial cell lines

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Marlène Dezest ◽  
Laurent Chavatte ◽  
Marion Bourdens ◽  
Damien Quinton ◽  
Mylène Camus ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Human Epithelial Cell ◽  
Pressure Plasma ◽  
Epithelial Cell Lines ◽  
Cold Atmospheric Pressure Plasma ◽  
The Impact

scholarly journals Inactivation by helium cold atmospheric pressure plasma for Escherichia coli and Staphylococcus aureus

2019 ◽  
Vol 14 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Khaled Lotfy ◽  
Sayed Mohammed Khalil ◽  
Hany Abd El-Raheem
Keyword(s):  
Flow Rate ◽  
Atmospheric Pressure ◽  
Plasma Temperature ◽  
Wavelength Region ◽  
Atmospheric Pressure Plasma ◽  
Optical Emission ◽  
Reactive Species ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
The Impact

AbstractA helium cold atmospheric pressure plasma jet (HCAPPJ) driven by a commercial neon power supply was designed and utilized for inactivation bacteria. The generated reactive spices by HCAPPJ were investigated by optical emission spectroscopy. The reactive species of OH, OI, OI, N21+, N21+ and He were identified in the UV–Vis wavelength region. The reactive species was not detected between 200 nm and 300 nm, as the flow rate of helium gas increased that led to the plasma temperature reducing to a value near to the room temperature. In this work, we studied the impact of HCAPPJ on Gram-positive and Gram-negative bacteria. The survival amounts of the two types of bacteria were decreased vastly when the rate flow rate was equal to 10 L/min.

scholarly journals Inactivation of a Foodborne Norovirus Outbreak Strain with Nonthermal Atmospheric Pressure Plasma

mBio ◽  
2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Birte Ahlfeld ◽  
Yangfang Li ◽  
Annika Boulaaba ◽  
Alfred Binder ◽  
Ulrich Schotte ◽  
...  
Keyword(s):  
Viral Load ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Health Care Facilities ◽  
Clinical Strain ◽  
Pressure Plasma ◽  
Intact Virus ◽  
Cold Atmospheric Pressure Plasma ◽  
The Impact ◽  
Initial Starting

ABSTRACT  Human norovirus (NoV) is the most frequent cause of epidemic nonbacterial acute gastroenteritis worldwide. We investigated the impact of nonthermal or cold atmospheric pressure plasma (CAPP) on the inactivation of a clinical human outbreak NoV, GII.4. Three different dilutions of a NoV-positive stool sample were prepared and subsequently treated with CAPP for various lengths of time, up to 15 min. NoV viral loads were quantified by quantitative real-time reverse transcription PCR (RT-qPCR). Increased CAPP treatment time led to increased NoV reduction; samples treated for the longest time had the lowest viral load. From the initial starting quantity of 2.36 × 104 genomic equivalents/ml, sample exposure to CAPP reduced this value by 1.23 log10 and 1.69 log10 genomic equivalents/ml after 10 and 15 min, respectively (P < 0.01). CAPP treatment of surfaces carrying a lower viral load reduced NoV by at least 1 log10 after CAPP exposure for 2 min (P < 0.05) and 1 min (P < 0.05), respectively. Our results suggest that NoV can be inactivated by CAPP treatment. The lack of cell culture assays prevents our ability to estimate infectivity. It is possible that some detectable, intact virus particles were rendered noninfectious. We conclude that CAPP treatment of surfaces may be a useful strategy to reduce the risk of NoV transmission in crowded environments. IMPORTANCE  Human gastroenteritis is most frequently caused by noroviruses, which are spread person to person and via surfaces, often in facilities with crowds of people. Disinfection of surfaces that come into contact with infected humans is critical for the prevention of cross-contamination and further transmission of the virus. However, effective disinfection cannot be done easily in mass catering environments or health care facilities. We evaluated the efficacy of cold atmospheric pressure plasma, an innovative airborne disinfection method, on surfaces inoculated with norovirus. We used a clinically relevant strain of norovirus from an outbreak in Germany. Cold plasma was able to inactivate the virus on the tested surfaces, suggesting that this method could be used for continuous disinfection of contaminated surfaces. The use of a clinical strain of norovirus strengthens the reliability of our results as it is a strain relevant to outbreaks in humans.

scholarly journals Evaluation of the Impact of Cold Atmospheric Pressure Plasma on Soybean Seed Germination

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 177
Author(s):  
Renáta Švubová ◽  
Ľudmila Slováková ◽  
Ľudmila Holubová ◽  
Dominika Rovňanová ◽  
Eliška Gálová ◽  
...  
Keyword(s):  
Dna Damage ◽  
Seed Germination ◽  
Succinate Dehydrogenase ◽  
Atmospheric Pressure ◽  
Soybean Seed ◽  
Atmospheric Pressure Plasma ◽  
Ambient Air ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
The Impact

The present study aims to define the effects of Cold Atmospheric Pressure Plasma (CAPP) exposure on seed germination of an agriculturally important crop, soybean. Seed treatment with lower doses of CAPP generated in ambient air and oxygen significantly increased the activity of succinate dehydrogenase (Krebs cycle enzyme), proving the switching of the germinating seed metabolism from anoxygenic to oxygenic. In these treatments, a positive effect on seed germination was documented (the percentage of germination increased by almost 20% compared to the untreated control), while the seed and seedling vigour was also positively affected. On the other hand, higher exposure times of CAPP generated in a nitrogen atmosphere significantly inhibited succinate dehydrogenase activity, but stimulated lactate and alcohol dehydrogenase activities, suggesting anoxygenic metabolism. It was also found that plasma exposure caused a slight increment in the level of primary DNA damage in ambient air- and oxygen-CAPP treatments, and more significant DNA damage was found in nitrogen-CAPP treatments. Although a higher level of DNA damage was also detected in the negative control (untreated seeds), this might be associated with the age of seeds followed by their lower germination capacity (with the germination percentage reaching only about 60%).

Fabrication and Performance of a Multidischarge Cold-Atmospheric Pressure Plasma Array

2021 ◽  
pp. 1-8
Author(s):  
Kenneth A. Cornell ◽  
Amanda White ◽  
Adam Croteau ◽  
Jessica Carlson ◽  
Zeke Kennedy ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
And Performance

scholarly journals Cold atmospheric pressure plasma jets as sources of singlet delta oxygen for biomedical applications

2011 ◽  
Vol 109 (12) ◽  
pp. 123302 ◽  
Author(s):  
J. S. Sousa ◽  
K. Niemi ◽  
L. J. Cox ◽  
Q. Th. Algwari ◽  
T. Gans ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Biomedical Applications ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jets ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

Infection of Human Epithelial Cell Lines by Epstein-Barr Virus

ORL ◽  
1984 ◽  
Vol 46 (4) ◽  
pp. 195-201 ◽  
Author(s):  
Tom Takimoto ◽  
Kazuo Morishita ◽  
Ryozo Umeda ◽  
Hisashi Ogura ◽  
Motoichi Hatano
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Epstein Barr Virus ◽  
Human Epithelial Cell ◽  
Barr Virus ◽  
Epithelial Cell Lines ◽  
Epstein Barr
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