scholarly journals The effect of physiological levels of South African puff adder (Bitis arietans) snake venom on blood cells: an in vitro model

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Morné A. Strydom ◽  
Janette Bester ◽  
Sthembile Mbotwe ◽  
Etheresia Pretorius
Keyword(s):  
South African ◽  
Snake Venom ◽  
Blood Cells ◽  
In Vitro Model ◽  
Vitro Model

An in Vitro Model for Arsine Toxicity Using Isolated Red Blood Cells

1995 ◽  
Vol 25 (2) ◽  
pp. 302-306
Author(s):  
K. M. HATLELID ◽  
C. BRAILSFORD ◽  
D. E. CARTER
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Model ◽  
Vitro Model

THE EFFECT OF COD LIVER OIL INTAKE ON THE STIMULATION OF BLOOD CELLS

1987 ◽  
Author(s):  
J B Hansen ◽  
J O Olsen ◽  
L Wilagård ◽  
B Østerud
Keyword(s):  
Platelet Aggregation ◽  
Young Women ◽  
Whole Blood ◽  
Blood Cells ◽  
In Vitro Model ◽  
Cod Liver Oil ◽  
Blood Samples ◽  
Vitro Model ◽  
Stimulation Of

In an in vitro model, stimulation of blood cells with a low concentration of lipopolysaccharides (LPS) revealed differences between women and men that possibly could be an explanation to why young women have less coronary heart disease than men (see abstract Hansen et al. “A model to--”).This model was also used to study the effect of intake of cod liver oil (CLO). 40 students (20 men and 20 women) were tested followed by an intake of 25 ml CLO daily for 2 months by 20 of the students.Heparinized blood samples were incubated with 2 ng LPS/ ml for 2 hours followed by isolation of plasma for thromboxane B2 and 6-keto-PG 1α quantitation.After the first 2 months period of CLO drinking we have the following results:The two months of CLO intake had no significant effect pn the thromboplastin induced synthesis in monocytes. In addition platelet aggregation was tested in a whole blood aggregometer using ADP addition to heparinized blood or collagen induced platelet aggregation in citrated whole blood. ADP aggregation was reduced from 75.9 ± 16.8% to 55.4 ± 19% in the CLO group of women, whereas the reduction in the CLO group of men was 70.1 ± 17.1% to 60.9±18.6%. Similar result were found with collagen aggregation (57% to 33% for women and 48% to 30% for men).It is concluded that CLO intake reduces TxA2 production and plateletaggregation without having reduced effect on PGI2 production in whole blood.

scholarly journals Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children

2010 ◽  
Vol 7 (1) ◽  
pp. 28 ◽  
Author(s):  
Georges JA Casimir ◽  
Fabienne Heldenbergh ◽  
Laurence Hanssens ◽  
Sandra Mulier ◽  
Claudine Heinrichs ◽  
...  
Keyword(s):  
Gender Differences ◽  
Blood Cells ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Red blood cells as an efficient in vitro model for evaluating the efficacy of metallic nanoparticles

3 Biotech ◽  
2019 ◽  
Vol 9 (7) ◽  
Author(s):  
Ridhima Wadhwa ◽  
Taru Aggarwal ◽  
Noopur Thapliyal ◽  
Ashutosh Kumar ◽  
Priya ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Metallic Nanoparticles ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals An In Vivo and In Vitro Model of Plasmodium falciparum Rosetting and Autoagglutination Mediated by varO, a Group A var Gene Encoding a Frequent Serotype

2008 ◽  
Vol 76 (12) ◽  
pp. 5565-5580 ◽  
Author(s):  
Inès Vigan-Womas ◽  
Micheline Guillotte ◽  
Cécile Le Scanf ◽  
Sébastien Igonet ◽  
Stéphane Petres ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Confidence Interval ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Model ◽  
Human Erythrocytes ◽  
Group A ◽  
Vitro Model

ABSTRACTIn theSaimiri sciureusmonkey, erythrocytes infected with the varO antigenic variant of thePlasmodium falciparumPalo Alto 89F5 clone bind uninfected red blood cells (rosetting), form autoagglutinates, and have a high multiplication rate, three phenotypic characteristics that are associated with severe malaria in human patients. We report here that varO parasites express avargene having the characteristics of group Avargenes, and we show that the varO Duffy binding-like 1α1(DBL1α1) domain is implicated in the rosetting of bothS. sciureusand human erythrocytes. The soluble varO N-terminal sequence (NTS)-DBL1α1recombinant domain, produced in a baculovirus-insect cell system, induced high titers of antibodies that reacted with varO-infected red blood cells and disrupted varO rosettes. varO parasites were culture adapted in vitro using human erythrocytes. They formed rosettes and autoagglutinates, and they had the same surface serotype and expressed the samevarOgene as the monkey-propagated parasites. To develop an in vitro model with highly homogeneous varO parasites, rosette purification was combined with positive selection by panning with a varO NTS-DBL1α1-specific mouse monoclonal antibody. The single-variant, clonal parasites were used to analyze seroprevalence for varO at the village level in a setting where malaria is holoendemic (Dielmo, Senegal). We found 93.6% (95% confidence interval, 89.7 to 96.4%) seroprevalence for varO surface-reacting antibodies and 86.7% (95% confidence interval, 82.8 to 91.6%) seroprevalence for the recombinant NTS-DBL1α1domain, and virtually all permanent residents had seroconverted by the age of 5 years. These data imply that the varO model is a relevant in vivo and in vitro model for rosetting and autoagglutination that can be used for rational development of vaccine candidates and therapeutic strategies aimed at preventing malaria pathology.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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