scholarly journals Influence of physical exercise on β-amyloid, α-synuclein and tau accumulation: an in vitro model of oxidative stress in human red blood cells

2017 ◽  
pp. 33-42
Author(s):  
Iofrida ◽  
S. Daniele ◽  
D. Pietrobono
Keyword(s):  
Oxidative Stress ◽  
Physical Exercise ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Model ◽  
Human Red Blood Cells ◽  
Β Amyloid ◽  
Vitro Model

An in Vitro Model for Arsine Toxicity Using Isolated Red Blood Cells

1995 ◽  
Vol 25 (2) ◽  
pp. 302-306
Author(s):  
K. M. HATLELID ◽  
C. BRAILSFORD ◽  
D. E. CARTER
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Red blood cells as an efficient in vitro model for evaluating the efficacy of metallic nanoparticles

3 Biotech ◽  
2019 ◽  
Vol 9 (7) ◽  
Author(s):  
Ridhima Wadhwa ◽  
Taru Aggarwal ◽  
Noopur Thapliyal ◽  
Ashutosh Kumar ◽  
Priya ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Metallic Nanoparticles ◽  
In Vitro Model ◽  
Vitro Model

Melatonin Ameliorates BPA Induced Oxidative Stress in Human Red Blood Cells: An In vitro Study

2020 ◽  
Vol 20 (8) ◽  
pp. 1321-1327
Author(s):  
Saleh M. Abdullah ◽  
Hina Rashid
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Study ◽  
Limited Data ◽  
Human Red Blood Cells ◽  
Living Organisms ◽  
Human Rbcs

Background: Bisphenol A (BPA) is a xenobiotic that causes oxidative stress in various organs in living organisms. Blood cells are also an endpoint where BPA is known to cause oxidative stress. Blood cells, especially red blood cells (RBCs), are crucial for maintaining homeostasis and overall wellbeing of the organism. They are highly susceptible to oxidative stress induced by xenobiotics. However, there is limited data about the oxidative stress induced by BPA in blood, especially in red blood cells. This study was carried out to evaluate BPA induced oxidative stress in human RBCs in vitro and its amelioration by melatonin. Objective: To find if melatonin exerts a protective effect on the oxidative stress induced by the BPA in human red blood cells in vitro. Methods: The erythrocyte suspensions (2 ml) were divided into six groups and treated with 0, 50, 100, 150, 200, and 250 μg/ml of BPA. Another set of erythrocyte suspension with similar BPA treatment and 50 μM Melatonin per group was also set. Incubations lasted for 12 hrs in the dark. Lipid peroxidation, glutathione, glutathione reductase, catalase, and superoxide dismutase were measured as indicators of oxidative stress. Results: BPA caused a significant increase in lipid peroxidation. A decrease in GSH levels was also observed. The activities of all the studied antioxidants also decreased with BPA treatment. Melatonin was seen to mitigate the oxidative stress induced by BPA. Conclusion: Treatment of red blood cells with BPA caused an increase in oxidative stress, while melatonin decreased the induced oxidative stress.

scholarly journals α-Synuclein Aggregates with β-Amyloid or Tau in Human Red Blood Cells: Correlation with Antioxidant Capability and Physical Exercise in Human Healthy Subjects

2017 ◽  
Vol 55 (3) ◽  
pp. 2653-2675 ◽  
Author(s):  
Simona Daniele ◽  
Deborah Pietrobono ◽  
Jonathan Fusi ◽  
Caterina Iofrida ◽  
Lucia Chico ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Healthy Subjects ◽  
Human Red Blood Cells ◽  
Β Amyloid

scholarly journals An In Vivo and In Vitro Model of Plasmodium falciparum Rosetting and Autoagglutination Mediated by varO, a Group A var Gene Encoding a Frequent Serotype

2008 ◽  
Vol 76 (12) ◽  
pp. 5565-5580 ◽  
Author(s):  
Inès Vigan-Womas ◽  
Micheline Guillotte ◽  
Cécile Le Scanf ◽  
Sébastien Igonet ◽  
Stéphane Petres ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Confidence Interval ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Model ◽  
Human Erythrocytes ◽  
Group A ◽  
Vitro Model

ABSTRACTIn theSaimiri sciureusmonkey, erythrocytes infected with the varO antigenic variant of thePlasmodium falciparumPalo Alto 89F5 clone bind uninfected red blood cells (rosetting), form autoagglutinates, and have a high multiplication rate, three phenotypic characteristics that are associated with severe malaria in human patients. We report here that varO parasites express avargene having the characteristics of group Avargenes, and we show that the varO Duffy binding-like 1α1(DBL1α1) domain is implicated in the rosetting of bothS. sciureusand human erythrocytes. The soluble varO N-terminal sequence (NTS)-DBL1α1recombinant domain, produced in a baculovirus-insect cell system, induced high titers of antibodies that reacted with varO-infected red blood cells and disrupted varO rosettes. varO parasites were culture adapted in vitro using human erythrocytes. They formed rosettes and autoagglutinates, and they had the same surface serotype and expressed the samevarOgene as the monkey-propagated parasites. To develop an in vitro model with highly homogeneous varO parasites, rosette purification was combined with positive selection by panning with a varO NTS-DBL1α1-specific mouse monoclonal antibody. The single-variant, clonal parasites were used to analyze seroprevalence for varO at the village level in a setting where malaria is holoendemic (Dielmo, Senegal). We found 93.6% (95% confidence interval, 89.7 to 96.4%) seroprevalence for varO surface-reacting antibodies and 86.7% (95% confidence interval, 82.8 to 91.6%) seroprevalence for the recombinant NTS-DBL1α1domain, and virtually all permanent residents had seroconverted by the age of 5 years. These data imply that the varO model is a relevant in vivo and in vitro model for rosetting and autoagglutination that can be used for rational development of vaccine candidates and therapeutic strategies aimed at preventing malaria pathology.

In vitro effects of low level yellow laser irradiation on human red blood cells

2016 ◽  
Author(s):  
Mustafa S. Al Musawi ◽  
M.S. Jaafar ◽  
B.T. Al-Gailani ◽  
Naser M. Ahmed ◽  
Fatanah M. Suhaimi
Keyword(s):  
Red Blood Cells ◽  
Laser Irradiation ◽  
Blood Cells ◽  
Low Level ◽  
Human Red Blood Cells ◽  
Yellow Laser ◽  
In Vitro Effects
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