scholarly journals Inhibition of Rac1 GTPase activity affects porcine oocyte maturation and early embryo development

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Si-Jing Song ◽  
Qiao-Chu Wang ◽  
Ru-Xia Jia ◽  
Xiang-Shun Cui ◽  
Nam-Hyung Kim ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Early Embryo ◽  
Gtpase Activity ◽  
Early Embryo Development ◽  
Porcine Oocyte ◽  
Rac1 Gtpase

scholarly journals Small GTPase RhoA regulates cytoskeleton dynamics during porcine oocyte maturation and early embryo development

Cell Cycle ◽  
2014 ◽  
Vol 13 (21) ◽  
pp. 3390-3403 ◽  
Author(s):  
Yu Zhang ◽  
Xing Duan ◽  
Rui Cao ◽  
Hong-Lin Liu ◽  
Xiang-Shun Cui ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Early Embryo ◽  
Small Gtpase ◽  
Early Embryo Development ◽  
Porcine Oocyte ◽  
Cytoskeleton Dynamics

Sonic hedgehog promotes porcine oocyte maturation and early embryo development

2009 ◽  
Vol 21 (6) ◽  
pp. 805 ◽  
Author(s):  
Ngoc Tan Nguyen ◽  
David Pei-Cheng Lin ◽  
Shih-Ying Yen ◽  
Jung-Kai Tseng ◽  
Jui-Fen Chuang ◽  
...  
Keyword(s):  
Embryo Development ◽  
Sonic Hedgehog ◽  
Oocyte Maturation ◽  
Calcium Release ◽  
Cyclin B1 ◽  
Early Embryo ◽  
Control Group ◽  
Early Embryo Development ◽  
Porcine Oocyte

In the present study, we investigated the effects of the Sonic hedgehog (Shh) protein on porcine oocyte maturation and early embryo development. Immunohistochemistry showed activation of Shh signalling in cumulus–oocyte complexes (COCs), as reflected by Patched (Ptc), Smoothened (Smo) and Gli1 expression in oocytes, cumulus cells and granulosa cells, particularly those of small follicles (<2 mm in diameter). Western blot analysis showed Smo expression in COCs and in denuded oocytes derived from small and medium (3–7 mm)-sized follicles. Small follicles contained the highest concentration of Shh in follicular fluid compared with medium-sized and large (>7 mm in diameter) follicles. Supplementation with Shh (0.5 or 1 μg mL–1) enhanced oocyte maturation compared with the control group (92.4% and 90.4% v. 81.9%, respectively; P < 0.05). This effect was reversed by the simultaneous addition of cyclopamine (1–2 μm), an Shh inhibitor. Similar to intact COCs, denuded COCs showed enhanced maturation following Shh supplementation. Furthermore, cyclin B1 content, extracellular signal-regulated kinase 1/2 phosphorylation, intracellular calcium release, blastocyst rate and total cell numbers were greater (P < 0.05) in oocytes matured in the presence of 0.5 and 1 μg mL–1 Shh compared with control oocytes. The findings of the present study provide the first evidence that the Shh signalling pathway is active, or at least partially activated, in the porcine ovary and is likely to promote oocyte cytoplasmic and nuclear maturation, as well as subsequent in vitro development, although the underlying mechanisms remain to be elucidated.

Sonic Hedgehog Supplementation Promotes Porcine Oocyte Maturation and Early Embryo Development.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 94-94
Author(s):  
Nguyen Ngoc Tan ◽  
Tzu-An Lin ◽  
Shi-Ing Yen ◽  
Jung-Kai Tseng ◽  
Rui-Fen Zhuang ◽  
...  
Keyword(s):  
Embryo Development ◽  
Sonic Hedgehog ◽  
Oocyte Maturation ◽  
Early Embryo ◽  
Early Embryo Development ◽  
Porcine Oocyte

83 MicroRNA574-3p Influences Porcine Oocyte Maturation and Regulates Abundance of Proteins Critical to Early Embryo Development

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 108-108
Author(s):  
Malavika K Adur ◽  
Yunsheng Li ◽  
Jason W Ross
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Protein Biosynthesis ◽  
Early Embryo ◽  
Control Group ◽  
Blastocyst Development ◽  
Study Objective ◽  
Early Embryo Development ◽  
Porcine Oocyte

Abstract MicroRNA are small non-coding RNA involved in post-transcriptional gene regulation impacting oocyte maturation and embryo development. MIR574-3p abundance decreases during oocyte in vitro maturation (IVM) and blastocyst development. The study objective was to evaluate the role of MIR574-3p during porcine oocyte maturation and early embryo development. To assess the function of MIR574-3p during these processes, denuded GV stage oocytes injected with MIR574-3p mimic (MIR574-3p-M), MIR574-3p inhibitor (MIR574-3p-I) or negative control oligo (NC) underwent IVM for 42 hours. The number of MII arrested oocytes was decreased (P = 0.06) in the MIR574-3p-M group (67.7 ± 1.4%) as compared to the NC group (76.1 ± 1.3%), whereas maturation was not affected by MIR574-3p-I (75.6 ± 1.5%) as compared to the NC group (73.1 ± 3.6%). MII arrested oocytes were parthenogenetically activated and cultured for 7 days. Neither mimic nor inhibitor affected the cleavage or blastocyst rate. Using LC-MS/MS we evaluated changes in global protein abundance in injected oocytes after 42 hours of IVM. We identified 971 proteins in MIR574-3p-M injected oocytes, of which 57 were differentially abundant as compared to the control group. In MIR574-3p-I injected oocytes, 1007 proteins were identified, of which 107 were differentially abundant as compared to the control group. Overall, MIR574-3p-M upregulated proteins critical to membrane binding mediating sperm receptors on the zona pellucida, while it downregulated intranuclear activity such as nucleotide biosynthesis, mitotic spindle assembly and orientation; whereas MIR574-3p-I induced upregulation of proteins involved in the processes between and including protein biosynthesis and metabolism, while downregulating proteins critical to ATP, RNA, DNA and protein binding. These data suggest artificially increasing MIR574-3p abundance during IVM alters the oocyte proteome and influences meiotic progression to MII. Project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2017-67015-26459 from the USDA National Institute of Food and Agriculture.

scholarly journals KIF20A Regulates Porcine Oocyte Maturation and Early Embryo Development

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e102898 ◽  
Author(s):  
Yu Zhang ◽  
Jun Liu ◽  
Xu Peng ◽  
Cheng-Cheng Zhu ◽  
Jun Han ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Early Embryo ◽  
Early Embryo Development ◽  
Porcine Oocyte
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