scholarly journals High minimum inhibitory concentration of imipenem as a predictor of fatal outcome in patients with carbapenem non-susceptible Klebsiella pneumoniae

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Ping-Feng Wu ◽  
Chien Chuang ◽  
Chin-Fang Su ◽  
Yi-Tsung Lin ◽  
Yu-Jiun Chan ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Klebsiella Pneumoniae ◽  
Inhibitory Concentration ◽  
Fatal Outcome ◽  
High Minimum Inhibitory Concentration

scholarly journals Developing an in silico minimum inhibitory concentration panel test for Klebsiella pneumoniae

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Marcus Nguyen ◽  
Thomas Brettin ◽  
S. Wesley Long ◽  
James M. Musser ◽  
Randall J. Olsen ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Klebsiella Pneumoniae ◽  
In Silico ◽  
Inhibitory Concentration ◽  
Panel Test

scholarly journals Klebsiella pneumoniae Planktonic and Biofilm Reduction by Different Plant Extracts: In Vitro Study

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Lucas Paula-Ramos ◽  
Carlos Eduardo da Rocha Santos ◽  
Daphne Camargo Reis Mello ◽  
Lígia Nishiama Theodoro ◽  
Felipe Eduardo De Oliveira ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Klebsiella Pneumoniae ◽  
Inhibitory Concentration ◽  
Juglans Regia ◽  
In Vitro Study ◽  
Chlorhexidine Digluconate ◽  
Planktonic Form ◽  
Microdilution Broth

This study evaluated the action of Pfaffia paniculata K., Juglans regia L., and Rosmarius officinalis L. extracts against planktonic form and biofilm of Klebsiella pneumoniae (ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at 107 UFC/mL of K. pneumoniae was distributed into 96-well microplates (n=10) and after 48 h at 37°C and biofilm was subjected to treatment for 5 min with the extracts at a concentration of 200 mg/mL. ANOVA and Tukey tests (5%) were used to verify statistical significant reduction (p<0.05) of planktonic form and biofilm. P paniculata K., R. officinalis L., and J. regia L. showed reductions in biomass of 55.6, 58.1, and 18.65% and cell viability reduction of 72.4, 65.1, and 31.5%, respectively. The reduction obtained with P. paniculata and R. officinalis extracts was similar to the reduction obtained with chlorhexidine digluconate 2%. In conclusion, all extracts have microbicidal action on the planktonic form but only P. paniculata K. and R. officinalis L. were effective against biofilm.

scholarly journals A Catheter-Related Bloodstream Infection Caused by the Yeast Kodamaea ohmeri

2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Ding CH ◽  
Tzar MN ◽  
Biswas S ◽  
Muttaqillah NAS ◽  
Wahab AA
Keyword(s):  
Diabetes Mellitus ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Bloodstream Infections ◽  
Blood Cultures ◽  
Initial Management ◽  
Kodamaea Ohmeri ◽  
High Minimum Inhibitory Concentration ◽  
Relative Scarcity ◽  
Catheter Related Bloodstream Infection

Catheter-related bloodstream infections caused by Kodamaea ohmeri are generally not considered due to the relative scarcity of reported cases. This is a case of an 85-year-old man with poorly controlled diabetes mellitus who was initially admitted to our hospital for diabetic ketoacidosis. An internal jugular catheter was inserted as part of the initial management. A week later the patient developed a temperature spike and a yeast identified as Kodamaea ohmeri by ID 32 C (bioMérieux, France) was isolated from both his central and peripheral blood cultures. The catheter was removed and the patient was treated with fluconazole despite the organism’s relatively high minimum inhibitory concentration (2 μg/mL) to this antifungal. The fungemia resolved following a 2-weeks course of fluconazole.

scholarly journals Sigmoid E max Modelling to Define the Fixed Concentration of Enmetazobactam for MIC Testing in Combination with Cefepime

2021 ◽  
Author(s):  
Philipp Knechtle ◽  
Stuart Shapiro ◽  
Ian Morrissey ◽  
Cyntia De Piano ◽  
Adam Belley
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Klebsiella Pneumoniae ◽  
Inhibitory Concentration ◽  
Carbapenem Resistance ◽  
Clinical Isolates ◽  
Extended Spectrum ◽  
Fixed Concentration

Use of carbapenem antibiotics to treat infections caused by Enterobacterales expressing increasingly aggressive extended-spectrum β-lactamases (ESBL) has contributed to the emergence of carbapenem resistance. Enmetazobactam is a novel ESBL inhibitor being developed in combination with cefepime as a carbapenem-sparing option for infections caused by ESBL-producing Enterobacterales . Cefepime-enmetazobactam checkerboard minimum inhibitory concentration (MIC) profiles were obtained for a challenge panel of cefepime-resistant ESBL-producing clinical isolates of Klebsiella pneumoniae . Sigmoid E max modelling described cefepime MIC as a function of enmetazobactam concentration with no bias. A concentration of 8 μg/ml enmetazobactam proved sufficient to restore >95% of cefepime antibacterial activity in vitro against >95% of isolates tested. These results support a fixed concentration of 8 μg/ml of enmetazobactam for MIC testing.

scholarly journals Evaluación de la capacidad bactericida de extractos vegetales de distinta polaridad de Drimys granadensis

2019 ◽  
Vol 26 (1) ◽  
pp. 135-142 ◽  
Author(s):  
Daniela Cuervo Salcedo ◽  
Julián Vanegas Campo ◽  
Diana Corzo Barragán ◽  
Felipe Correa Mahecha
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Klebsiella Pneumoniae ◽  
Inhibitory Concentration ◽  
Staphylococcus Epidermis ◽  
Concentration Test ◽  
Para Determinar

Empleando el método de maceración en frío y fraccionamiento con solventes de polaridad creciente, se obtuvo cuatro extractos vegetales de distinta polaridad en las hojas de Drimys granadensis: Muy apolar (MA), apolar (A), polar (P) y Muy polar (MP), los cuales se obtuvieron al utilizar hexano, cloroformo, acetona y metanol para el fraccionamiento correspondiente. Una vez se obtuvieron los extractos, se siguió el protocolo de Minimum Inhibitory Concentration test (MIC) para determinar la concentración mínima a la cual se inhibe el crecimiento bacteriano, frente a dos cepas bacterianas Gram positivas: Staphylococcus aureus y Staphylococcus epidermis; y dos Gram negativas: Klebsiella pneumoniae y Escherichia coli. Como resultado se obtuvo que la fracción polar (P) fue la más efectiva, inhibiendo el crecimiento de todas las cepas bacterianas evaluadas a partir de una concentración de 15 mg/mL.

scholarly journals A Nanoparticles based Microbiological Study on the Effect of Rosemary and Ginger Essential Oils against Klebsiella pneumoniae.

2020 ◽  
Vol 14 (1) ◽  
pp. 205-212
Author(s):  
Rania Abozahra ◽  
Sarah M. Abdelhamid ◽  
Ming Ming Wen ◽  
Ibrahim Abdelwahab ◽  
Kholoud Baraka
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Klebsiella Pneumoniae ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Chitosan Nanoparticles ◽  
Nanostructured Lipid Carrier ◽  
Isolation And Identification ◽  
Biofilm Inhibition ◽  
Broth Microdilution Method ◽  
Rosemary Oil

Background: Klebsiella pneumoniae is a nosocomial pathogen in outbreaks of hospital infections. It is one of the major factors for morbidity and mortality in hospitalized patients especially those infected with colistin-resistant pathogens. Many plant essential oils have antimicrobial activities and have been investigated as natural sources to combat multiple antibiotic resistances. Moreover, recent advances in phytonanotechnology have created exciting opportunities for the management of many infections. Objective: This study aims at investigating the antimicrobial and antibiofilm effect of rosemary and ginger essential oil-based nano-sized formulations on colistin resistant K. pneumonia clinical isolates. Methods: Isolation and identification of 30 K. pneumonia isolates from different human samples were done followed by antibiotic susceptibility testing and detection of biofilm gene (mrkD). Examination of the activity of the tested essential oils and their chitosan nanoparticle formulations against the selected isolates was made by determination of their MICs using broth microdilution method followed by biofilm inhibition test and quantitative real-time PCR for the expression of mrkD gene in the presence of the oils and nanoparticles formulations compared to untreated bacterial isolates. Results: Our results showed that the minimum inhibitory concentration of rosemary and ginger oils was 1250 μg/ml, that of nanostructured lipid carrier-rosemary oil and nanostructured lipid carrier-ginger oil was 625 μg/ml and rosemary oil loaded chitosan nanoparticles and ginger oil loaded chitosan nanoparticles possessed minimum inhibitory concentration of 156 μg/ml. Results also revealed complete (100%) inhibition for mrkD gene expression when compared to untreated K. pneumonia. Conclusion: Oil loaded chitosan nanoparticles showed the highest antimicrobial and antibiofilm activity.

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