scholarly journals Ribonuclease 5 facilitates corneal endothelial wound healing via activation of PI3-kinase/Akt pathway

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Kyoung Woo Kim ◽  
Soo Hyun Park ◽  
Soo Jin Lee ◽  
Jae Chan Kim
Keyword(s):  
Wound Healing ◽  
Pi3 Kinase ◽  
Akt Pathway ◽  
Endothelial Wound Healing

scholarly journals eG Coated Stents Exhibit Enhanced Endothelial Wound Healing Characteristics

2021 ◽  
Author(s):  
Belen Rodriguez-Garcia ◽  
Christophe Bureau ◽  
Abdul I. Barakat
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Bare Metal Stents ◽  
Automated Image Analysis ◽  
Butyl Methacrylate ◽  
Metal Stents ◽  
Cobalt Chromium ◽  
Endothelial Lining ◽  
Stent Deployment ◽  
Endothelial Wound Healing

Abstract Purpose Despite their widespread use, a significant fraction of coronary stents suffer from in-stent restenosis and stent thrombosis. Stent deployment induces extensive injury to the vascular endothelium. Rapid endothelial wound closure is essential for the success of a stenting procedure. A recent study has demonstrated that the BuMA Supreme® sirolimus-eluting stent exhibits particularly attractive strut coverage characteristics. A unique feature of this stent is the presence of a thin brush layer of poly-butyl methacrylate (PBMA), covalently bonded to the stent’s cobalt-chromium frame via electro-grafting (eG™). The present study aimed to determine whether the PBMA coating has an effect on endothelial cell wound healing and stent strut coverage. Methods We used an in vitro coronary artery model whose wall consisted of an annular collagen hydrogel and whose luminal surface was lined with a monolayer of endothelial cells. Mechanical wounding of the endothelial lining was preformed prior to deployment of a bare cobalt-chromium stent either with or without the PBMA layer. The migration of fluorescently labeled endothelial cells was monitored automatically over a period of 48 h to determine endothelial wound healing rates. Results Quantitative assessment of endothelial wound healing rates within the simulated arterial model is achievable using automated image analysis. Wound healing is significantly faster (44% faster at 48 h) for stents with the PBMA eG Coating™ compared to bare metal stents. Conclusion The PBMA eG Coating™ has the effect of promoting endothelial wound healing. Future studies will focus on elucidating the mechanistic basis of this observation.

In Vivo Confocal Microscopic Studies of Endothelial Wound Healing in Rabbit Cornea

Cornea ◽  
1993 ◽  
Vol 12 (5) ◽  
pp. 369-378 ◽  
Author(s):  
Hideji Ichijima ◽  
W Matthew Petroll ◽  
James V. Jester ◽  
Patricia A. Barry ◽  
Peter M. Andrews ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rabbit Cornea ◽  
Microscopic Studies ◽  
Endothelial Wound Healing

Endurance exercise training increases insulin responsiveness in isolated adipocytes through IRS/PI3-kinase/Akt pathway

2005 ◽  
Vol 98 (3) ◽  
pp. 1037-1043 ◽  
Author(s):  
Sidney B. Peres ◽  
Solange M. Franzói de Moraes ◽  
Cecilia E. M. Costa ◽  
Luciana C. Brito ◽  
Julie Takada ◽  
...  
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Exercise Training ◽  
Insulin Receptor ◽  
Endurance Exercise ◽  
Pi3 Kinase ◽  
Akt Pathway ◽  
Β Subunit ◽  
Endurance Exercise Training ◽  
Isolated Adipocytes

Endurance exercise training promotes important metabolic adaptations, and the adipose tissue is particularly affected. The aim of this study was to investigate how endurance exercise training modulates some aspects of insulin action in isolated adipocytes and in intact adipose tissue. Male Wistar rats were submitted to daily treadmill running (1 h/day) for 7 wk. Sedentary age-matched rats were used as controls. Final body weight, body weight gain, and epididymal fat pad weight did not show any statistical differences between groups. Adipocytes from trained rats were smaller than those from sedentary rats (205 ± 16.8 vs. 286 ± 26.4 pl; P < 0.05). Trained rats showed decreased plasma glucose (4.9 ± 0.13 vs. 5.3 ± 0.07 mM; P < 0.05) and insulin levels (0.24 ± 0.012 vs. 0.41 ± 0.049 mM; P < 0.05) and increased insulin-stimulated glucose uptake (23.1 ± 3.1 vs. 12.1 ± 2.9 pmol/cm2; P < 0.05) compared with sedentary rats. The number of insulin receptors and the insulin-induced tyrosine phosphorylation of insulin receptor-β subunit did not change between groups. Insulin-induced tyrosine phosphorylation insulin receptor substrates (IRS)-1 and -2 increased significantly (1.57- and 2.38-fold, respectively) in trained rats. Insulin-induced IRS-1/phosphatidylinositol 3 (PI3)-kinase (but not IRS-2/PI3-kinase) association and serine Akt phosphorylation also increased (2.06- and 3.15-fold, respectively) after training. The protein content of insulin receptor-β subunit, IRS-1 and -2, did not differ between groups. Taken together, these data support the hypothesis that the increased adipocyte responsiveness to insulin observed after endurance exercise training is modulated by IRS/PI3-kinase/Akt pathway.

scholarly journals Glucagon-like peptide-1 analogue liraglutide facilitates wound healing by activating PI3K/Akt pathway in keratinocytes

2018 ◽  
Vol 146 ◽  
pp. 155-161 ◽  
Author(s):  
Konosuke Nagae ◽  
Hiroshi Uchi ◽  
Saori Morino-Koga ◽  
Yuka Tanaka ◽  
Mari Oda ◽  
...  
Keyword(s):  
Wound Healing ◽  
Akt Pathway ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

High Kpnβ1 expression promotes non-small cell lung cancer proliferation and chemoresistance via the PI3-kinase/AKT pathway

2018 ◽  
Vol 51 ◽  
pp. 39-48 ◽  
Author(s):  
Haiying Wang ◽  
Danping Wang ◽  
Chunsun Li ◽  
Xingsong Zhang ◽  
Xiaolin Zhou ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Pi3 Kinase ◽  
Akt Pathway ◽  
Small Cell Lung ◽  
Cancer Proliferation

scholarly journals A genetic variant of cortactin linked to acute lung injury impairs lamellipodia dynamics and endothelial wound healing

2015 ◽  
Vol 309 (9) ◽  
pp. L983-L994 ◽  
Author(s):  
Sangwook Choi ◽  
Sara M. Camp ◽  
Arkaprava Dan ◽  
Joe G. N. Garcia ◽  
Steven M. Dudek ◽  
...  
Keyword(s):  
Wound Healing ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Regulatory Protein ◽  
Actin Binding ◽  
Gap Closure ◽  
Sphingosine 1 Phosphate ◽  
Endothelial Wound Healing ◽  
Genetic Signatures ◽  
The Impact

Inflammatory mediators released in acute lung injury (ALI) trigger the disruption of interendothelial junctions, leading to loss of vascular barrier function, protein-rich pulmonary edema, and severe hypoxemia. Genetic signatures that predict patient recovery or disease progression are poorly defined, but recent genetic screening of ALI patients has identified an association between lung inflammatory disease and a single nucleotide polymorphism (SNP) in the gene for the actin-binding and barrier-regulatory protein cortactin. This study investigated the impact of this disease-linked cortactin variant on wound healing processes that may contribute to endothelial barrier restoration. A microfabricated platform was used to quantify wound healing in terms of gap closure speed, lamellipodia dynamics, and cell velocity. Overexpression of wild-type cortactin in endothelial cells (ECs) improved directional cell motility and enhanced lamellipodial protrusion length, resulting in enhanced gap closure rates. By contrast, the cortactin SNP impaired wound closure and cell locomotion, consistent with the observed reduction in lamellipodial protrusion length and persistence. Overexpression of the cortactin SNP in lung ECs mitigated the barrier-enhancing activity of sphingosine 1-phosphate. These findings suggest that this common cortactin variant may functionally contribute to ALI predisposition by impeding endothelial wound healing.

Establish an In Vitro Cell Model to Explore the Impacts of UVA on Human Corneal Endothelial Wound Healing

2020 ◽  
Vol 45 (9) ◽  
pp. 1065-1073
Author(s):  
Guo-Jian Jiang ◽  
Ying Li ◽  
Xin-Guo You ◽  
Ting-Jun Fan
Keyword(s):  
Wound Healing ◽  
Cell Model ◽  
Endothelial Wound Healing
Sign in / Sign up

Export Citation Format

Share Document