scholarly journals In-situ Isotopic Analysis at Nanoscale using Parallel Ion Electron Spectrometry: A Powerful New Paradigm for Correlative Microscopy

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Lluís Yedra ◽  
Santhana Eswara ◽  
David Dowsett ◽  
Tom Wirtz
Keyword(s):  
Isotopic Analysis ◽  
Correlative Microscopy ◽  
New Paradigm ◽  
Electron Spectrometry

Laboratory and in-situ analysis of comet dust

1988 ◽  
Vol 46 ◽  
pp. 8-9
Author(s):  
D.E. Brownlee ◽  
A.L. Albee
Keyword(s):  
Electron Microscopy ◽  
Solar System ◽  
Laboratory Study ◽  
Isotopic Analysis ◽  
Building Blocks ◽  
Sem Analysis ◽  
Early Solar System ◽  
Cometary Material ◽  
Comet Dust

Comets are primitive, kilometer-sized bodies that formed in the outer regions of the solar system. Composed of ice and dust, comets are generally believed to be relic building blocks of the outer solar system that have been preserved at cryogenic temperatures since the formation of the Sun and planets. The analysis of cometary material is particularly important because the properties of cometary material provide direct information on the processes and environments that formed and influenced solid matter both in the early solar system and in the interstellar environments that preceded it.The first direct analyses of proven comet dust were made during the Soviet and European spacecraft encounters with Comet Halley in 1986. These missions carried time-of-flight mass spectrometers that measured mass spectra of individual micron and smaller particles. The Halley measurements were semi-quantitative but they showed that comet dust is a complex fine-grained mixture of silicates and organic material. A full understanding of comet dust will require detailed morphological, mineralogical, elemental and isotopic analysis at the finest possible scale. Electron microscopy and related microbeam techniques will play key roles in the analysis. The present and future of electron microscopy of comet samples involves laboratory study of micrometeorites collected in the stratosphere, in-situ SEM analysis of particles collected at a comet and laboratory study of samples collected from a comet and returned to the Earth for detailed study.

scholarly journals Lifestyle of sponge symbiont phages by host prediction and correlative microscopy

2021 ◽  
Author(s):  
M. T. Jahn ◽  
T. Lachnit ◽  
S. M. Markert ◽  
C. Stigloher ◽  
L. Pita ◽  
...  
Keyword(s):  
Distinct Pattern ◽  
Microbial Consortia ◽  
Bacterial Symbionts ◽  
Correlative Microscopy ◽  
Phage Ecology ◽  
Infection Dynamics ◽  
Imaging Approach ◽  
Animal Hosts ◽  
Viral Distribution

AbstractBacteriophages (phages) are ubiquitous elements in nature, but their ecology and role in animals remains little understood. Sponges represent the oldest known extant animal-microbe symbiosis and are associated with dense and diverse microbial consortia. Here we investigate the tripartite interaction between phages, bacterial symbionts, and the sponge host. We combined imaging and bioinformatics to tackle important questions on who the phage hosts are and what the replication mode and spatial distribution within the animal is. This approach led to the discovery of distinct phage-microbe infection networks in sponge versus seawater microbiomes. A new correlative in situ imaging approach (‘PhageFISH-CLEM‘) localised phages within bacterial symbiont cells, but also within phagocytotically active sponge cells. We postulate that the phagocytosis of free virions by sponge cells modulates phage-bacteria ratios and ultimately controls infection dynamics. Prediction of phage replication strategies indicated a distinct pattern, where lysogeny dominates the sponge microbiome, likely fostered by sponge host-mediated virion clearance, while lysis dominates in seawater. Collectively, this work provides new insights into phage ecology within sponges, highlighting the importance of tripartite animal-phage-bacterium interplay in holobiont functioning. We anticipate that our imaging approach will be instrumental to further understanding of viral distribution and cellular association in animal hosts.

In situ isotopic analysis of uranium using a new data acquisition protocol for 1013 ohm Faraday amplifiers

2021 ◽  
Author(s):  
Kota Yamamoto ◽  
Hisashi Asanuma ◽  
Hiroaki Takahashi ◽  
Takafumi Hirata
Keyword(s):  
Data Acquisition ◽  
Data Reduction ◽  
Reduction Method ◽  
Isotopic Analysis ◽  
High Gain ◽  
Transient Signals ◽  
Acquisition Protocol ◽  
Data Reduction Method ◽  
Isotopic Measurements

New data reduction method for isotopic measurements using high-gain Faraday amplifiers enables precise uranium isotopic analysis even from transient signals.

Aqueous Dispersion Polymerization: A New Paradigm for in Situ Block Copolymer Self-Assembly in Concentrated Solution

2011 ◽  
Vol 133 (39) ◽  
pp. 15707-15713 ◽  
Author(s):  
Shinji Sugihara ◽  
Adam Blanazs ◽  
Steven P. Armes ◽  
Anthony J. Ryan ◽  
Andrew L. Lewis
Keyword(s):  
Block Copolymer ◽  
Self Assembly ◽  
Dispersion Polymerization ◽  
Aqueous Dispersion ◽  
New Paradigm ◽  
Concentrated Solution

Investigation of Pt Bottom Electrodes for "In-Situ" Deposited Pb(Zr,Ti)O3 (PZT) thin Films

1991 ◽  
Vol 243 ◽  
Author(s):  
Rainer Bruchhaus ◽  
Dana Pitzer ◽  
Oliver Eibl ◽  
Uwe Scheithauer ◽  
Wolfgang Hoesler
Keyword(s):  
Elevated Temperatures ◽  
Diffusion Processes ◽  
Rutherford Backscattering Spectrometry ◽  
Layer Sequence ◽  
Si Substrates ◽  
Transmission Electron ◽  
In Situ Deposition ◽  
Electron Spectrometry ◽  
Heating Up

AbstractThe deposition of the bottom electrode plays a key role in the fabrication of ferroelectric capacitors. Processing at elevated temperatures of up to 800°C can give rise to diffusion processes and thereof formation of harmful dielectric layers.In this paper we used Rutherford backscattering spectrometry (RBS), Auger electron spectrometry (AES) and transmission electron microscopy (TEM) to study Pt/Ti/SiO2/Si substrates with various thicknesses of the Ti and Pt layers. During heating up to about 450°C in vacuum the initial layer sequence remains unchanged. However, drastic changes occur when the electrodes are exposed to Ar/O2 atmosphere during heat treatment. Oxidation induced diffusion of Ti into Pt and oxidation of Ti were observed. A Pt electrode with a 100 nm thick Ti adhesion layer proved to be suitable for the "in-situ" deposition of PZT films.

scholarly journals Distributed Immersive Participation as Crowd-Sensing in Culture Events

2014 ◽  
Vol 7 (2) ◽  
Author(s):  
Theo Kanter ◽  
Rahim Rahmani ◽  
Jamie Walters ◽  
Willmar Sauter
Keyword(s):  
Internet Of Things ◽  
Distributed Computing ◽  
Real Time ◽  
Social Innovation ◽  
The Internet ◽  
New Paradigm ◽  
Content Creation ◽  
Crowd Sensing ◽  
Information Objects

This article investigates new forms for creating and enabling massive and scalable participatory immersive experiences in live cultural events, characterized by processes, involving pervasive objects, places and people. The multi-disciplinary research outlines a new paradigm for collaborative creation and participation towards technological and social innovation, tapping into crowd-sensing. The approach promotes user-driven content-creation and offsets economic models thereby rewarding creators and performers. In response to these challenges, we propose a framework for bringing about massive and real-time presence and awareness on the Internet through an Internet-of-Things infrastructure to connect artifacts, performers, participants and places. Equally importantly, we enable the in-situ creation of collaborative experiences building on relevant existing and stored content, based on decisions leveraging multi-criteria clustering and proximity of pervasive information, objects, people and places. Finally, we investigate some new ways for immersive experiences via distributed computing but pointing forward to the necessity to do more with regard to collaborative creation.

Sign in / Sign up

Export Citation Format

Share Document