scholarly journals Novel role of cortactin in G protein-coupled receptor agonist-induced nuclear export and degradation of p21Cip1

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jagadeesh Janjanam ◽  
Gadiparthi N. Rao
Keyword(s):  
G Protein ◽  
Nuclear Export ◽  
Receptor Agonist ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Role of Detergents in Conformational Exchange of a G Protein-coupled Receptor

2012 ◽  
Vol 287 (43) ◽  
pp. 36305-36311 ◽  
Author(s):  
Ka Young Chung ◽  
Tae Hun Kim ◽  
Aashish Manglik ◽  
Rohan Alvares ◽  
Brian K. Kobilka ◽  
...  
Keyword(s):  
G Protein ◽  
Conformational Exchange ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

The G protein coupled receptor 55 in the human placenta and the role of its endogenous agonist lysophosphatidylinositol (LPI) on endothelial function

Placenta ◽  
2013 ◽  
Vol 34 (9) ◽  
pp. A18-A19
Author(s):  
Julia Kremshofer ◽  
Gerit Moser ◽  
Monika Siwetz ◽  
Astrid Blaschitz ◽  
Monika Sundl ◽  
...  
Keyword(s):  
Endothelial Function ◽  
G Protein ◽  
Human Placenta ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Role of the G-Protein-Coupled Receptor Signaling Pathway in Insecticide Resistance

2019 ◽  
Vol 20 (17) ◽  
pp. 4300 ◽  
Author(s):  
Ting Li ◽  
Nannan Liu
Keyword(s):  
Insecticide Resistance ◽  
Cell Line ◽  
Signaling Pathway ◽  
G Protein ◽  
Cell Lines ◽  
Sf9 Cells ◽  
Camp Production ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

The G-protein-coupled receptor (GPCR) regulated intracellular signaling pathway is known to be involved in the development of insecticide resistance in the mosquito, Culex quinquefasciatus. To elucidate the specific role of each effector in the GPCR regulating pathway, we initially expressed a GPCR, G-protein alpha subunit (Gαs), adenylate cyclase (AC), and protein kinase A (PKA) in insect Spodoptera frugiperda (Sf9) cells and investigated their regulation function on cyclic AMP (cAMP) production and PKA activity. GPCR, Gαs, and AC individually expressed Sf9 cells showed higher cAMP production as the expression of each effector increased. All the effector-expressed cell lines showed increased PKA activity however. Moreover, Sf9 cytochrome P450 gene expression and cell tolerance to permethrin were examined. The relative expression of CYP9A32gene in Sf9 cells tested was significantly increased in all effector-expressed cell lines compared to a control cell line; these effector-expressed cell lines also showed significantly higher tolerance to permethrin. Inhibitor treatments on each effector-expressed cell line revealed that Bupivacaine HCl and H89 2HCl robustly inhibited cAMP production and PKA activity, respectively, resulting in decreased tolerance to permethrin in all cell lines. The synergistic functions of Bupivacaine HCl and H89 2HCl with permethrin were further examined in Culex mosquito larvae, providing a valuable new information for mosquito control strategies.

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