scholarly journals Haem-assisted dityrosine-cross-linking of fibrinogen under non-thermal plasma exposure: one important mechanism of facilitated blood coagulation

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Zhigang Ke ◽  
Qing Huang
Keyword(s):  
Blood Coagulation ◽  
Thermal Plasma ◽  
Cross Linking ◽  
Plasma Exposure ◽  
Non Thermal Plasma

Surface characterization of Ti and Y-TZP following non-thermal plasma exposure

2011 ◽  
Vol 99B (1) ◽  
pp. 199-206 ◽  
Author(s):  
Nelson R.F.A. Silva ◽  
Paulo G. Coelho ◽  
Guilherme B. Valverde ◽  
Kurt Becker ◽  
Roland Ihrke ◽  
...  
Keyword(s):  
Thermal Plasma ◽  
Surface Characterization ◽  
Plasma Exposure ◽  
Non Thermal Plasma

scholarly journals Inactivation of Acanthamoeba Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma

2021 ◽  
Vol 9 (9) ◽  
pp. 1879
Author(s):  
Tereza Měřínská ◽  
Vladimír Scholtz ◽  
Josef Khun ◽  
Jaroslav Julák ◽  
Eva Nohýnková
Keyword(s):  
Water Content ◽  
Thermal Plasma ◽  
Contact Lenses ◽  
Optical Power ◽  
Infrared And Raman Spectra ◽  
Plasma Exposure ◽  
Water Suspension ◽  
Complete Inactivation ◽  
Water Suspensions ◽  
Non Thermal Plasma

Water suspensions of cysts of a pathogenic clinical isolate of Acanthamoeba sp. were prepared, and the cysts were inactivated either in suspension or placed on the surface of contact lenses by the non-thermal plasma produced by the DC corona transient spark discharge. The efficacy of this treatment was determined by cultivation and the presence of vegetative trophozoites indicating non-inactivated cysts. The negative discharge appeared to be more effective than the positive one. The complete inactivation occurred in water suspension after 40 min and on contaminated lenses after 50 min of plasma exposure. The properties of lenses seem to not be affected by plasma exposure; that is, their optical power, diameter, curvature, water content and infrared and Raman spectra remain unchanged.

Mechanism of Blood Coagulation by Non-Thermal Atmospheric Pressure Dielectric Barrier Discharge Plasma.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3162-3162 ◽  
Author(s):  
Gayathri Nagaraj ◽  
Manjula Balasubramanian ◽  
Sameer Kalghatgi ◽  
Andrew S. Wu ◽  
Ari D. Brooks ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Platelet Activation ◽  
Thermal Plasma ◽  
Atmospheric Pressure ◽  
Discharge Plasma ◽  
Barrier Discharge ◽  
Dielectric Barrier Discharge Plasma ◽  
Dielectric Barrier ◽  
Barrier Discharge Plasma ◽  
Non Thermal Plasma

Abstract Introduction: Non-thermal atmospheric pressure dielectric barrier discharge plasma (cold plasma due to its non-thermal nature) has emerged as a promising new tool in medicine due to its ability to coagulate blood rapidly, sterilize tissue without thermal damage and induce apoptosis in malignant tissue. The potential clinical applicability of non-thermal plasma lies in its use in controlling intra-operative microvascular bleeding in organs and in endoscopy. Non-thermal plasma can also be used to treat superficial wounds in trauma through hemostasis while simultaneously inducing surface sterilization. We have previously demonstrated that non-thermal plasma hastens blood coagulation on cut tissue surfaces and accelerates clot formation in whole blood five times faster than natural coagulation. A series of experiments were undertaken to investigate the mechanism of coagulation by non-thermal plasma. Methods/Results: We initially postulated that changes in pH and/or extracellular Ca2+ as a possible mechanism for non-thermal plasma mediated coagulation. Our studies however showed no significant changes in pH or Ca2+ in treated blood. Thermal energy triggered coagulation as seen in conventional electrocautery as well as electric field effects were eliminated as other possible mechanisms. The role of reactive oxygen species (ROS) in coagulation was studied, as non-thermal plasma is known to produce ROS in water. ROS production in blood was blocked with sodium pyruvate, an ROS scavenger, and the results showed no effect on non-thermal plasma induced coagulation. Specific effects of non-thermal plasma on citrated blood samples revealed extremely rapid coagulation with surface gel formation, while clotting studies (PT, aPTT) performed on the plasma beneath the gel revealed consumption of coagulation factors. Examination of the clot formed by non-thermal plasma using Scanning Electron Microscopy (SEM) showed platelet activation with pseudopodia formation, aggregation, and fibrin formation. The effects of non-thermal plasma on fibrinogen solution treated at physiologic pH showed a change in opacity suggesting clot formation. Dynamic Light Scattering (DLS) was used to measure particle size distributions of treated and untreated fibrinogen solutions. Treated fibrinogen exhibited a multi-modal distribution of sizes with the largest size corresponding to the size of fibrin-like structures. This suggests that non-thermal plasma may coagulate blood by the conversion of fibrinogen to fibrin. Evaluation of albumin, our control protein given its non-involvement in coagulation, showed no changes upon exposure to non-thermal plasma. Conclusion: Non-thermal plasma likely promotes coagulation by enhancing the physiologic coagulation process through direct activation of fibrinogen as well as platelet activation and aggregation. Future research will further evaluate the mechanisms of non-thermal plasma induced platelet activation and effects on other proteins in the coagulation cascade. This will lead to newer insights into the physiological aspects of coagulation and clinical utility of non-thermal plasma in medicine.

Atmospheric pressure non-thermal plasma exposure reduces Pseudomonas aeruginosa lipopolysaccharide toxicity in vitro and in vivo

2019 ◽  
Vol 136 ◽  
pp. 103679
Author(s):  
Muna M. Barakat ◽  
Yahya H. Dallal Bashi ◽  
Louise Carson ◽  
William G. Graham ◽  
Brendan F. Gilmore ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Thermal Plasma ◽  
Atmospheric Pressure ◽  
Plasma Exposure ◽  
Non Thermal Plasma ◽  
Toxicity In Vitro

scholarly journals Non-thermal Plasma Exposure Rapidly Attenuates Bacterial AHL-Dependent Quorum Sensing and Virulence

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Padrig B. Flynn ◽  
Alessandro Busetti ◽  
Ewa Wielogorska ◽  
Olivier P. Chevallier ◽  
Christopher T. Elliott ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Thermal Plasma ◽  
Plasma Exposure ◽  
Non Thermal Plasma

Use of non-thermal plasma in lignocellulosic materials: A smart alternative

2021 ◽  
Vol 109 ◽  
pp. 365-373
Author(s):  
Gabriela N. Pereira ◽  
Karina Cesca ◽  
Anelise Leal Vieira Cubas ◽  
Débora de Oliveira
Keyword(s):  
Thermal Plasma ◽  
Lignocellulosic Materials ◽  
Non Thermal Plasma
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