scholarly journals Intravenous administration of lidocaine directly acts on spinal dorsal horn and produces analgesic effect: An in vivo patch-clamp analysis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Miyuki Kurabe ◽  
Hidemasa Furue ◽  
Tatsuro Kohno
Keyword(s):  
Spinal Cord ◽  
Patch Clamp ◽  
Dorsal Horn ◽  
Acute Pain ◽  
Analgesic Effect ◽  
Spinal Dorsal Horn ◽  
Intravenous Lidocaine ◽  
Spinal Dorsal ◽  
Postsynaptic Currents

Abstract Intravenous lidocaine administration produces an analgesic effect in various pain states, such as neuropathic and acute pain, although the underlying mechanisms remains unclear. Here, we hypothesized that intravenous lidocaine acts on spinal cord neurons and induces analgesia in acute pain. We therefore examined the action of intravenous lidocaine in the spinal cord using the in vivo patch-clamp technique. We first investigated the effects of intravenous lidocaine using behavioural measures in rats. We then performed in vivo patch-clamp recording from spinal substantia gelatinosa (SG) neurons. Intravenous lidocaine had a dose-dependent analgesic effect on the withdrawal response to noxious mechanical stimuli. In the electrophysiological experiments, intravenous lidocaine inhibited the excitatory postsynaptic currents (EPSCs) evoked by noxious pinch stimuli. Intravenous lidocaine also decreased the frequency, but did not change the amplitude, of both spontaneous and miniature EPSCs. However, it did not affect inhibitory postsynaptic currents. Furthermore, intravenous lidocaine induced outward currents in SG neurons. Intravenous lidocaine inhibits glutamate release from presynaptic terminals in spinal SG neurons. Concomitantly, it hyperpolarizes postsynaptic neurons by shifting the membrane potential. This decrease in the excitability of spinal dorsal horn neurons may be a possible mechanism for the analgesic action of intravenous lidocaine in acute pain.

In Vivo Patch-Clamp Analysis of IPSCs Evoked in Rat Substantia Gelatinosa Neurons by Cutaneous Mechanical Stimulation

2000 ◽  
Vol 84 (4) ◽  
pp. 2171-2174 ◽  
Author(s):  
Keita Narikawa ◽  
Hidemasa Furue ◽  
Eiichi Kumamoto ◽  
Megumu Yoshimura
Keyword(s):  
Patch Clamp ◽  
Receptor Antagonist ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Substantia Gelatinosa ◽  
Mechanical Stimuli ◽  
Patch Clamp Technique ◽  
Spinal Dorsal ◽  
Postsynaptic Currents

To know a functional role of inhibitory synaptic responses in transmitting noxious and innoxious information from the periphery to the rat spinal dorsal horn, we examined inhibitory postsynaptic currents (IPSCs) elicited in substantia gelatinosa (SG) neurons by mechanical stimuli applied to the skin using the newly developed in vivo patch-clamp technique. In the majority (80%) of SG neurons examined, a brush stimulus applied to the ipsilateral hind limb produced a barrage of IPSCs that persisted during the stimulus, while a pinch stimulus evoked IPSCs only at its beginning and end. The pinch-evoked IPSCs may have been caused by a touch that occurs at the on/off time of the pinch. The evoked IPSCs were blocked by either a glycine-receptor antagonist, strychnine (4 μM), or a GABAA-receptor antagonist, bicuculline (20 μM). All SG neurons examined received inhibitory inputs from a wide area throughout the thigh and lower leg. When IPSCs were examined together with excitatory postsynaptic currents (EPSCs) in the same neurons, a brush evoked a persistent activity of both IPSCs and EPSCs during the stimulus while a pinch evoked such an activity of EPSCs but not IPSCs. It is suggested that innoxious mechanical stimuli activate a GABAergic or glycinergic circuitry in the spinal dorsal horn. This inhibitory transmission may play an important role in the modulation of noxious information in the SG.

scholarly journals Noradrenaline modulates mechanically evoked responses in the rat spinal dorsal horn: an in vivo patch-clamp study

2019 ◽  
Vol Volume 12 ◽  
pp. 1269-1278 ◽  
Author(s):  
Motoki Sonohata ◽  
Atsushi Doi ◽  
Toshiharu Yasaka ◽  
Daisuke Uta ◽  
Masaaki Mawatari ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Evoked Responses ◽  
Spinal Dorsal ◽  
Clamp Study

In vivo patch-clamp analysis of sensory neuronal circuit in the rat superficial spinal dorsal horn

2004 ◽  
Vol 1269 ◽  
pp. 69-72 ◽  
Author(s):  
Hidemasa Furue ◽  
Go Kato ◽  
Ji Hoon Kim ◽  
Byung-Il Min ◽  
Toshihiko Katafuchi ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Neuronal Circuit ◽  
Spinal Dorsal

306 IN VIVO IMAGING OF Ca 2+ GRADIENTS IN SPINAL DORSAL HORN NEURONS

2009 ◽  
Vol 13 (S1) ◽  
Author(s):  
M. Gassner ◽  
M. Wagner ◽  
H. Fischer ◽  
R. Drdla ◽  
T. Jäger ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
In Vivo Imaging ◽  
Spinal Dorsal Horn ◽  
Dorsal Horn Neurons ◽  
Spinal Dorsal ◽  
Spinal Dorsal Horn Neurons

scholarly journals TWIK-Related Spinal Cord K+Channel Expression Is Increased in the Spinal Dorsal Horn after Spinal Nerve Ligation

2015 ◽  
Vol 56 (5) ◽  
pp. 1307 ◽  
Author(s):  
Hee Youn Hwang ◽  
Enji Zhang ◽  
Sangil Park ◽  
Woosuk Chung ◽  
Sunyeul Lee ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
K Channel ◽  
Spinal Dorsal ◽  
Channel Expression

Measurement of cholecystokinin release in vivo in the rat spinal dorsal horn

1999 ◽  
Vol 4 (2) ◽  
pp. 192-200 ◽  
Author(s):  
Henrik Gustafsson ◽  
Guilherme de Araujo Lucas ◽  
Eva Schött ◽  
Carl-Olav Stiller ◽  
Pawel Alster ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Spinal Dorsal

Interleukin-1beta released by microglia initiates the enhanced glutamatergic activity in the spinal dorsal horn during paclitaxel-associated acute pain syndrome

Glia ◽  
2018 ◽  
Vol 67 (3) ◽  
pp. 482-497 ◽  
Author(s):  
Xisheng Yan ◽  
Fen Li ◽  
Dylan W. Maixner ◽  
Ruchi Yadav ◽  
Mei Gao ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Acute Pain ◽  
Spinal Dorsal Horn ◽  
Pain Syndrome ◽  
Spinal Dorsal ◽  
Interleukin 1Beta

scholarly journals Metabotropic glutamate receptor 5 regulates excitability and Kv4.2-containing K+ channels primarily in excitatory neurons of the spinal dorsal horn

2011 ◽  
Vol 105 (6) ◽  
pp. 3010-3021 ◽  
Author(s):  
Hui-Juan Hu ◽  
Robert W. Gereau
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Neuronal Excitability ◽  
Gabaergic Neurons ◽  
Erk Signaling ◽  
Metabotropic Glutamate ◽  
Dorsal Horn Neurons ◽  
Spinal Dorsal ◽  
Excitatory Neurons

Metabotropic glutamate (mGlu) receptors play important roles in the modulation of nociception. Previous studies demonstrated that mGlu5 modulates nociceptive plasticity via activation of ERK signaling. We have reported recently that the Kv4.2 K+ channel subunit underlies A-type currents in spinal cord dorsal horn neurons and that this channel is modulated by mGlu5-ERK signaling. In the present study, we tested the hypothesis that modulation of Kv4.2 by mGlu5 occurs in excitatory spinal dorsal horn neurons. With the use of a transgenic mouse strain expressing enhanced green fluorescent protein (GFP) under control of the promoter for the γ-amino butyric acid (GABA)-synthesizing enzyme, glutamic acid decarboxylase 67 (GAD67), we found that these GABAergic neurons express less Kv4.2-mediated A-type current than non-GAD67-GFP neurons. Furthermore, the mGlu1/5 agonist, (R,S)-3,5-dihydroxyphenylglycine, had no modulatory effects on A-type currents or neuronal excitability in this subgroup of GABAergic neurons but robustly modulated A-type currents and neuronal excitability in non-GFP-expressing neurons. Immunofluorescence studies revealed that Kv4.2 was highly colocalized with markers of excitatory neurons, such as vesicular glutamate transporter 1/2, PKCγ, and neurokinin 1, in cultured dorsal horn neurons. These results indicate that mGlu5-Kv4.2 signaling is associated with excitatory dorsal horn neurons and suggest that the pronociceptive effects of mGlu5 activation in the spinal cord likely involve enhanced excitability of excitatory neurons.

Sign in / Sign up

Export Citation Format

Share Document