In vivo patch-clamp analysis of sensory neuronal circuit in the rat superficial spinal dorsal horn

2004 ◽  
Vol 1269 ◽  
pp. 69-72 ◽  
Author(s):  
Hidemasa Furue ◽  
Go Kato ◽  
Ji Hoon Kim ◽  
Byung-Il Min ◽  
Toshihiko Katafuchi ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Neuronal Circuit ◽  
Spinal Dorsal

scholarly journals Noradrenaline modulates mechanically evoked responses in the rat spinal dorsal horn: an in vivo patch-clamp study

2019 ◽  
Vol Volume 12 ◽  
pp. 1269-1278 ◽  
Author(s):  
Motoki Sonohata ◽  
Atsushi Doi ◽  
Toshiharu Yasaka ◽  
Daisuke Uta ◽  
Masaaki Mawatari ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Evoked Responses ◽  
Spinal Dorsal ◽  
Clamp Study

In Vivo Patch-Clamp Analysis of IPSCs Evoked in Rat Substantia Gelatinosa Neurons by Cutaneous Mechanical Stimulation

2000 ◽  
Vol 84 (4) ◽  
pp. 2171-2174 ◽  
Author(s):  
Keita Narikawa ◽  
Hidemasa Furue ◽  
Eiichi Kumamoto ◽  
Megumu Yoshimura
Keyword(s):  
Patch Clamp ◽  
Receptor Antagonist ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Substantia Gelatinosa ◽  
Mechanical Stimuli ◽  
Patch Clamp Technique ◽  
Spinal Dorsal ◽  
Postsynaptic Currents

To know a functional role of inhibitory synaptic responses in transmitting noxious and innoxious information from the periphery to the rat spinal dorsal horn, we examined inhibitory postsynaptic currents (IPSCs) elicited in substantia gelatinosa (SG) neurons by mechanical stimuli applied to the skin using the newly developed in vivo patch-clamp technique. In the majority (80%) of SG neurons examined, a brush stimulus applied to the ipsilateral hind limb produced a barrage of IPSCs that persisted during the stimulus, while a pinch stimulus evoked IPSCs only at its beginning and end. The pinch-evoked IPSCs may have been caused by a touch that occurs at the on/off time of the pinch. The evoked IPSCs were blocked by either a glycine-receptor antagonist, strychnine (4 μM), or a GABAA-receptor antagonist, bicuculline (20 μM). All SG neurons examined received inhibitory inputs from a wide area throughout the thigh and lower leg. When IPSCs were examined together with excitatory postsynaptic currents (EPSCs) in the same neurons, a brush evoked a persistent activity of both IPSCs and EPSCs during the stimulus while a pinch evoked such an activity of EPSCs but not IPSCs. It is suggested that innoxious mechanical stimuli activate a GABAergic or glycinergic circuitry in the spinal dorsal horn. This inhibitory transmission may play an important role in the modulation of noxious information in the SG.

scholarly journals Intravenous administration of lidocaine directly acts on spinal dorsal horn and produces analgesic effect: An in vivo patch-clamp analysis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Miyuki Kurabe ◽  
Hidemasa Furue ◽  
Tatsuro Kohno
Keyword(s):  
Spinal Cord ◽  
Patch Clamp ◽  
Dorsal Horn ◽  
Acute Pain ◽  
Analgesic Effect ◽  
Spinal Dorsal Horn ◽  
Intravenous Lidocaine ◽  
Spinal Dorsal ◽  
Postsynaptic Currents

Abstract Intravenous lidocaine administration produces an analgesic effect in various pain states, such as neuropathic and acute pain, although the underlying mechanisms remains unclear. Here, we hypothesized that intravenous lidocaine acts on spinal cord neurons and induces analgesia in acute pain. We therefore examined the action of intravenous lidocaine in the spinal cord using the in vivo patch-clamp technique. We first investigated the effects of intravenous lidocaine using behavioural measures in rats. We then performed in vivo patch-clamp recording from spinal substantia gelatinosa (SG) neurons. Intravenous lidocaine had a dose-dependent analgesic effect on the withdrawal response to noxious mechanical stimuli. In the electrophysiological experiments, intravenous lidocaine inhibited the excitatory postsynaptic currents (EPSCs) evoked by noxious pinch stimuli. Intravenous lidocaine also decreased the frequency, but did not change the amplitude, of both spontaneous and miniature EPSCs. However, it did not affect inhibitory postsynaptic currents. Furthermore, intravenous lidocaine induced outward currents in SG neurons. Intravenous lidocaine inhibits glutamate release from presynaptic terminals in spinal SG neurons. Concomitantly, it hyperpolarizes postsynaptic neurons by shifting the membrane potential. This decrease in the excitability of spinal dorsal horn neurons may be a possible mechanism for the analgesic action of intravenous lidocaine in acute pain.

306 IN VIVO IMAGING OF Ca 2+ GRADIENTS IN SPINAL DORSAL HORN NEURONS

2009 ◽  
Vol 13 (S1) ◽  
Author(s):  
M. Gassner ◽  
M. Wagner ◽  
H. Fischer ◽  
R. Drdla ◽  
T. Jäger ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
In Vivo Imaging ◽  
Spinal Dorsal Horn ◽  
Dorsal Horn Neurons ◽  
Spinal Dorsal ◽  
Spinal Dorsal Horn Neurons

Measurement of cholecystokinin release in vivo in the rat spinal dorsal horn

1999 ◽  
Vol 4 (2) ◽  
pp. 192-200 ◽  
Author(s):  
Henrik Gustafsson ◽  
Guilherme de Araujo Lucas ◽  
Eva Schött ◽  
Carl-Olav Stiller ◽  
Pawel Alster ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Spinal Dorsal

scholarly journals Evaluation of Formalin-Induced Pain Behavior and Glutamate Release in the Spinal Dorsal Horn Using In Vivo Microdialysis in Conscious Rats

2012 ◽  
Vol 120 (2) ◽  
pp. 129-132 ◽  
Author(s):  
Alba Vidal-Torres ◽  
Alicia Carceller ◽  
Daniel Zamanillo ◽  
Manuel Merlos ◽  
Jos^|^eacute; Miguel Vela ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Glutamate Release ◽  
In Vivo Microdialysis ◽  
Pain Behavior ◽  
Conscious Rats ◽  
Spinal Dorsal

scholarly journals Inhibitory action of nociceptin on spinal dorsal horn neurones of the rat, in vivo

1996 ◽  
Vol 118 (8) ◽  
pp. 1875-1877 ◽  
Author(s):  
L.C. Stanfa ◽  
V. Chapman ◽  
N. Kerr ◽  
A.H. Dickenson
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Inhibitory Action ◽  
Spinal Dorsal ◽  
Dorsal Horn Neurones

scholarly journals Neuron Type-Dependent Synaptic Activity in the Spinal Dorsal Horn of Opioid-Induced Hyperalgesia Mouse Model

2021 ◽  
Vol 13 ◽  
Author(s):  
Austin Kearns ◽  
Jazmine Jayasi ◽  
Xin Liu ◽  
Jigong Wang ◽  
Yuqiang Shi ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Pain Sensitivity ◽  
Specific Cell ◽  
Dependent Manner ◽  
Neuronal Circuit ◽  
Morphine Treatment ◽  
Tonic Firing ◽  
Spinal Dorsal ◽  
Opioid Induced Hyperalgesia

Opioids are widely used for pain relief; however, chronic opioid use causes a paradoxical state of enhanced pain sensitivity, termed “Opioid-induced hyperalgesia (OIH).” Despite the clinical importance of OIH, the detailed mechanism by which it enhances pain sensitivity remains unclear. In this study, we tested whether repeated morphine induces a neuronal circuit polarization in the mouse spinal dorsal horn (SDH). Transgenic mice expressing GFP to neurokinin 1 receptor-expressing neurons (sNK1Rn) and GABAergic interneurons (sGABAn) that received morphine [20 mg/kg, once daily for four consecutive days (i.p.)] developed mechanical hypersensitivity. Repeated morphine altered synaptic strengths in the SDH as a specific cell-type but not in a gender-dependent manner. In sNK1Rn and non-tonic firing neurons, repeated morphine treatment significantly increased frequency of spontaneous excitatory postsynaptic current (sEPSC) and evoked EPSC (eEPSC). In addition, repeated morphine treatment significantly decreased evoked inhibitory postsynaptic current (eIPSC) in sNK1Rn. Conversely, in sGABAn and tonic firing neurons, repeated morphine treatment significantly decreased sEPSC frequency and eEPSC, but had no change of eIPSC in sGABAn. Interestingly, repeated morphine treatment significantly decreased neuronal rheobase of sNK1Rn but had no effect on sGABAn. These findings suggest that spinal neuronal circuit polarization maybe the mechanism of OIH and identify a potential therapeutic mechanism to prevent or treat opioid-induced pain.

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