scholarly journals Chitosan oligosaccharide induces resistance to Tobacco mosaic virus in Arabidopsis via the salicylic acid-mediated signalling pathway

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xiaochen Jia ◽  
Qingshan Meng ◽  
Haihong Zeng ◽  
Wenxia Wang ◽  
Heng Yin
Keyword(s):  
Salicylic Acid ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Signalling Pathway ◽  
Chitosan Oligosaccharide

scholarly journals Salicylic Acid and Jasmonic Acid Are Essential for Systemic Resistance Against Tobacco mosaic virus in Nicotiana benthamiana

2014 ◽  
Vol 27 (6) ◽  
pp. 567-577 ◽  
Author(s):  
Feng Zhu ◽  
De-Hui Xi ◽  
Shu Yuan ◽  
Fei Xu ◽  
Da-Wei Zhang ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Foliar Application ◽  
Mass Spectrometry Analysis ◽  
Systemic Resistance ◽  
Long Distance ◽  
Resistance Response

Systemic resistance is induced by pathogens and confers protection against a broad range of pathogens. Recent studies have indicated that salicylic acid (SA) derivative methyl salicylate (MeSA) serves as a long-distance phloem-mobile systemic resistance signal in tobacco, Arabidopsis, and potato. However, other experiments indicate that jasmonic acid (JA) is a critical mobile signal. Here, we present evidence suggesting both MeSA and methyl jasmonate (MeJA) are essential for systemic resistance against Tobacco mosaic virus (TMV), possibly acting as the initiating signals for systemic resistance. Foliar application of JA followed by SA triggered the strongest systemic resistance against TMV. Furthermore, we use a virus-induced gene-silencing–based genetics approach to investigate the function of JA and SA biosynthesis or signaling genes in systemic response against TMV infection. Silencing of SA or JA biosynthetic and signaling genes in Nicotiana benthamiana plants increased susceptibility to TMV. Genetic experiments also proved the irreplaceable roles of MeSA and MeJA in systemic resistance response. Systemic resistance was compromised when SA methyl transferase or JA carboxyl methyltransferase, which are required for MeSA and MeJA formation, respectively, were silenced. Moreover, high-performance liquid chromatography–mass spectrometry analysis indicated that JA and MeJA accumulated in phloem exudates of leaves at early stages and SA and MeSA accumulated at later stages, after TMV infection. Our data also indicated that JA and MeJA could regulate MeSA and SA production. Taken together, our results demonstrate that (Me)JA and (Me)SA are required for systemic resistance response against TMV.

Salicylic Acid Interferes with Tobacco Mosaic Virus Replication via a Novel Salicylhydroxamic Acid-Sensitive Mechanism

1997 ◽  
Vol 9 (4) ◽  
pp. 547 ◽  
Author(s):  
Stephen Chivasa ◽  
Alex M. Murphy ◽  
Martin Naylor ◽  
John P. Carr
Keyword(s):  
Salicylic Acid ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Replication ◽  
Salicylhydroxamic Acid

scholarly journals Effects of DICER-like proteins 2, 3 and 4 on cucumber mosaic virus and tobacco mosaic virus infections in salicylic acid-treated plants

2009 ◽  
Vol 90 (12) ◽  
pp. 3010-3014 ◽  
Author(s):  
Mathew G. Lewsey ◽  
John P. Carr
Keyword(s):  
Salicylic Acid ◽  
Tobacco Mosaic Virus ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Rna Viruses ◽  
Virus Infections ◽  
Defence Mechanisms ◽  
Triple Mutant ◽  
Positive Sense

Salicylic acid (SA)-mediated resistance and RNA silencing are both important plant antiviral defence mechanisms. To investigate overlap between these resistance phenomena, we examined the ability of mutant Arabidopsis thaliana plants lacking DICER-like (DCL) endoribonucleases 2, 3 and 4 to exhibit SA-induced defence. We found that in dcl2/3/4 triple mutant plants, treatment with exogenous SA stimulated resistance to two positive-sense RNA viruses: cucumber mosaic virus and tobacco mosaic virus. We conclude that DCLs 2, 3 and 4, which are the predominant DCL endoribonucleases involved in silencing of positive-sense RNA viruses, are not required for effective SA-induced resistance to these viruses. However, the findings do not exclude RNA silencing from making a contribution to SA-mediated resistance in wild-type plants.

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