Cyanide Restores N Gene-Mediated Resistance to Tobacco Mosaic Virus in Transgenic Tobacco Expressing Salicylic Acid Hydroxylase

1998 ◽  
Vol 10 (9) ◽  
pp. 1489 ◽  
Author(s):  
Stephen Chivasa ◽  
John P. Carr
Keyword(s):  
Salicylic Acid ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
N Gene

scholarly journals Novel N Gene-Associated, Temperature-Independent Resistance to the Movement of Tobacco Mosaic Virus Vectors Neutralized by a Cucumber Mosaic Virus RNA1 Transgene

2002 ◽  
Vol 76 (24) ◽  
pp. 12908-12916 ◽  
Author(s):  
Tomas Canto ◽  
Peter Palukaitis
Keyword(s):  
Salicylic Acid ◽  
Green Fluorescent Protein ◽  
Tobacco Mosaic Virus ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Fluorescent Protein ◽  
N Gene ◽  
Nicotiana Glutinosa ◽  
Tobacco Plants ◽  
Green Fluorescent

ABSTRACT The N gene conditions for resistance to Tobacco mosaic virus (TMV) but only below 28°C. However, a TMV-based vector expressing green fluorescent protein (TMV-GFP) showed only limited movement at 33°C in tobacco plants harboring the N gene and other genes cointrogressed from Nicotiana glutinosa. TMV-GFP moved efficiently in tobacco plants that either lacked these genes or that contained the N gene but were transgenic for RNA1 of Cucumber mosaic virus. These findings identified novel temperature-independent resistance to the movement of TMV-GFP which could be neutralized by a different viral transgene. Using the N gene and nahG gene-transgenic tobacco, we show that this novel resistance is manifested specifically by the N gene itself and operates via a pathway independent of salicylic acid.

scholarly journals Transgenic Plants Expressing Potato Virus X ORF2 Protein (p24) Are Resistant to Tobacco Mosaic Virus and Ob Tobamoviruses

1998 ◽  
Vol 72 (1) ◽  
pp. 731-738 ◽  
Author(s):  
X. Ares ◽  
G. Calamante ◽  
S. Cabral ◽  
J. Lodge ◽  
P. Hemenway ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Potato Virus ◽  
Potato Virus X ◽  
N Gene ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Virus Accumulation

ABSTRACT The p24 protein, one of the three proteins implicated in local movement of potato virus X (PVX), was expressed in transgenic tobacco plants (Nicotiana tabacum Xanthi D8 NN). Plants with the highest level of p24 accumulation exhibited a stunted and slightly chlorotic phenotype. These transgenic plants facilitate the cell-to-cell movement of a mutant of PVX that contained a frameshift mutation in p24. Upon inoculation with tobacco mosaic virus (TMV), the size of necrotic local lesions was significantly smaller in p24+ plants than in nontransgenic, control plants. Systemic resistance to tobamoviruses was also evidenced after inoculation of p24+ plants with Ob, a virus that evades the hypersensitive response provided by the N gene. In the latter case, no systemic symptoms were observed, and virus accumulation remained low or undetectable by Western immunoblot analysis and back-inoculation assays. In contrast, no differences were observed in virus accumulation after inoculation with PVX, although more severe symptoms were evident on p24-expressing plants than on control plants. Similarly, infection assays conducted with potato virus Y showed no differences between control and transgenic plants. On the other hand, a considerable delay in virus accumulation and symptom development was observed when transgenic tobacco plants containing the movement protein (MP) of TMV were inoculated with PVX. Finally, a movement defective mutant of TMV was inoculated on p24+ plants or in mixed infections with PVX on nontransgenic plants. Both types of assays failed to produce TMV infections, implying that TMV MP is not interchangeable with the PVX MPs.

Systemic infection of some N-gene-carrying Nicotiana species and cultivars after inoculation with tobacco mosaic virus

1977 ◽  
Vol 83 (2) ◽  
pp. 41-59 ◽  
Author(s):  
Jeanne Dijkstra ◽  
G. C. A. Bruin ◽  
Ankie C. Burgers ◽  
L. C. Loon ◽  
Christien Ritter ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Systemic Infection ◽  
N Gene ◽  
Nicotiana Species

Hypersensitivity to Tobacco Mosaic Virus in N’-Gene Hosts: Which Viral Genes are Involved?

1990 ◽  
pp. 345-359 ◽  
Author(s):  
K. W. Mundry ◽  
W. Schaible ◽  
M. Ellwart-Tschürtz ◽  
H. Nitschko ◽  
C. Hapke
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
N Gene ◽  
Viral Genes
Sign in / Sign up

Export Citation Format

Share Document