scholarly journals In vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Won Hyuk Jang ◽  
Sehwan Shim ◽  
Taejun Wang ◽  
Yeoreum Yoon ◽  
Won-Suk Jang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Early Stage ◽  
Skin Injury ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
In Vivo Characterization

scholarly journals In vivo imaging of activated microglia in a mouse model of focal cerebral ischemia by two-photon microscopy

2015 ◽  
Vol 6 (9) ◽  
pp. 3303 ◽  
Author(s):  
Seoyeon Bok ◽  
Taejun Wang ◽  
Chan-Ju Lee ◽  
Seong-Uk Jeon ◽  
Young-Eun Kim ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Mouse Model ◽  
In Vivo Imaging ◽  
Focal Cerebral Ischemia ◽  
Activated Microglia ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals In Vivo Characterization of Changing Blood-Tumor Barrier Permeability in a Mouse Model of Breast Cancer Metastasis

2011 ◽  
Vol 46 (11) ◽  
pp. 718-725 ◽  
Author(s):  
Dean B. Percy ◽  
Emeline J. Ribot ◽  
Yuhua Chen ◽  
Catherine McFadden ◽  
Carmen Simedrea ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mouse Model ◽  
Cancer Metastasis ◽  
Breast Cancer Metastasis ◽  
Barrier Permeability ◽  
In Vivo Characterization

scholarly journals Longitudinal In Vivo Characterization of the Streptozotocin-Induced Diabetic Mouse Model: Focus on Early Inner Retinal Responses

2019 ◽  
Vol 60 (2) ◽  
pp. 807 ◽  
Author(s):  
Jurgen Sergeys ◽  
Isabelle Etienne ◽  
Inge Van Hove ◽  
Evy Lefevere ◽  
Ingeborg Stalmans ◽  
...  
Keyword(s):  
Mouse Model ◽  
Diabetic Mouse ◽  
In Vivo Characterization

scholarly journals In vivo two-photon microscopy reveals the contribution of Sox9+ cell to kidney regeneration in a mouse model with extracellular vesicle treatment

2020 ◽  
Vol 295 (34) ◽  
pp. 12203-12213 ◽  
Author(s):  
Kaiyue Zhang ◽  
Shang Chen ◽  
Huimin Sun ◽  
Lina Wang ◽  
Huifang Li ◽  
...  
Keyword(s):  
Mouse Model ◽  
Kidney Injury ◽  
Extracellular Vesicle ◽  
Lineage Tracing ◽  
Abdominal Imaging ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Activation Mechanisms ◽  
Renal Regeneration

Mesenchymal stem cell (MSC)–derived extracellular vesicles (EVs) have been shown to stimulate regeneration in the treatment of kidney injury. Renal regeneration is also thought to be stimulated by the activation of Sox9+ cells. However, whether and how the activation mechanisms underlying EV treatment and Sox9+ cell–dependent regeneration intersect is unclear. We reasoned that a high-resolution imaging platform in living animals could help to untangle this system. To test this idea, we first applied EVs derived from human placenta–derived MSCs (hP-MSCs) to a Sox9-CreERT2; R26mTmG transgenic mouse model of acute kidney injury (AKI). Then, we developed an abdominal imaging window in the mouse and tracked the Sox9+ cells in the inducible Sox9-Cre transgenic mice via in vivo lineage tracing with two-photon intravital microscopy. Our results demonstrated that EVs can travel to the injured kidneys post intravenous injection as visualized by Gaussia luciferase imaging and markedly increase the activation of Sox9+ cells. Moreover, the two-photon living imaging of lineage-labeled Sox9+ cells showed that the EVs promoted the expansion of Sox9+ cells in kidneys post AKI. Histological staining results confirmed that the descendants of Sox9+ cells contributed to nephric tubule regeneration which significantly ameliorated the renal function after AKI. In summary, intravital lineage tracing with two-photon microscopy through an embedded abdominal imaging window provides a practical strategy to investigate the beneficial functions and to clarify the mechanisms of regenerative therapies in AKI.

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