scholarly journals In vitro follicle growth supports human oocyte meiotic maturation

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Shuo Xiao ◽  
Jiyang Zhang ◽  
Megan M. Romero ◽  
Kristin N. Smith ◽  
Lonnie D. Shea ◽  
...  
Keyword(s):  
Meiotic Maturation ◽  
Human Oocyte ◽  
Follicle Growth ◽  
Oocyte Meiotic Maturation

Diethylstilbestrol exposure disrupts mouse oocyte meiotic maturation in vitro through affecting spindle assembly and chromosome alignment

Chemosphere ◽  
2020 ◽  
Vol 249 ◽  
pp. 126182 ◽  
Author(s):  
Zhi-Ming Ding ◽  
Li-Ping Hua ◽  
Muhammad Jamil Ahmad ◽  
Muhammad Safdar ◽  
Fan Chen ◽  
...  
Keyword(s):  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Maturation In Vitro ◽  
Chromosome Alignment ◽  
Oocyte Meiotic Maturation

Bisphenol A (BPA) Exposure and Human Oocyte Meiotic Maturation.

2012 ◽  
Vol 87 (Suppl_1) ◽  
pp. 122-122 ◽  
Author(s):  
Ronit Machtinger ◽  
Russ Hauser ◽  
Stacey A. Missmer ◽  
Katharine F. Correia ◽  
Catherine Combelles ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Meiotic Maturation ◽  
Human Oocyte ◽  
Oocyte Meiotic Maturation

The impact of bisphenol A (BPA) on human oocyte meiotic maturation

2011 ◽  
Vol 96 (3) ◽  
pp. S7 ◽  
Author(s):  
R. Machtinger ◽  
R. Hauser ◽  
C. Combelles ◽  
C. Racowsky
Keyword(s):  
Bisphenol A ◽  
Meiotic Maturation ◽  
Human Oocyte ◽  
Oocyte Meiotic Maturation ◽  
The Impact

Gamma Tubulin is Localized to a New Organelle, The Multivesicular Aggregate, In Mammalian Oocytes

1998 ◽  
Vol 4 (S2) ◽  
pp. 1124-1125
Author(s):  
Patricia G. Calarco
Keyword(s):  
Chorionic Gonadotropin ◽  
Immunofluorescence Microscopy ◽  
Germinal Vesicle ◽  
Central Nucleus ◽  
Meiotic Maturation ◽  
Follicle Growth ◽  
Early Maturation ◽  
Nuclear Envelope Breakdown ◽  
Gamma Tubulin ◽  
Oocyte Meiotic Maturation

Oocyte meiotic maturation occurs without the aid of centrioles in the mouse; instead the division spindles are organized by a number of microtubule organizing centers (MTOC). Immunofluorescence microscopy using anti-γ tubulin shows that multiple MTOCs migrate to surround the central nucleus (germinal vesicle, GV) during early maturation.. However, at the EM level, recognizable MTOCs have never been seen prior to the nucleation of microtubules (MT) which occurs near the time of nuclear envelope breakdown. Recently, I described multivesicular aggregates (MVAs) as the organelles most likely to represent immature MTOCs prior to MT nucleation4. These are first seen at time zero by EM as two large aggregates in the cortex, with sizes averaging 10um. MVA subsequently fragment into smaller units as they migrate centrally.Materials and Methods: Female ICR mice were injected IP with 100 iu of chorionic gonadotropin (Sigma) to stimulate follicle growth, and ovaries were removed 48 hours later.

200 SYNCHRONIZATION OF OOCYTE MEIOTIC MATURATION IN SUPEROVULATED MICE IMPROVES IN VITRO FERTILIZATION RATE

2012 ◽  
Vol 24 (1) ◽  
pp. 212
Author(s):  
A. M. Taiyeb Ridha ◽  
D. C. Kraemer
Keyword(s):  
Embryonic Development ◽  
Fertilization Rate ◽  
Early Embryonic Development ◽  
Meiotic Maturation ◽  
Control Group ◽  
Vitro Fertilization ◽  
Development Rates ◽  
Oocyte Meiotic Maturation

In vitro synchronization of oocyte nuclear and cytoplasmic maturation has been found to improve the IVF rate of ovarian oocytes in several species, including humans, in comparison with nonsynchronized in vitro-matured oocytes. Here, we tested the hypothesis that synchronization of oocyte meiotic maturation by an in vivo system in superovulated mice would increase the oocyte fertilization rate when compared to that of conventional superovulated oocytes. Recently, we observed that cilostazol (CZL), a PDE3-I, was able to inhibit mouse oocyte meiotic maturation in both in vitro and in vivo systems. Administering CZL at 7.5 mg, 4 or 7 h pre-hCG allowed retrieval of ovulated oocytes of which >95% were at MI stage, scored by Nikon stereo microscope (SMZ 1500). A conventional superovulation program was adapted in all treated and their control groups, in which mice were injected with eCG and after 48 h with hCG (7.5 IU for each hormone). On the second morning, 13 to 14 h post-hCG, mice were killed and oocytes were collected from oviducts and in vitro fertilized (control). For the treated groups, CZL was administered in a single 7.5 mg oral dose (gavage) 4 or 7 h before the hCG injection. On the second morning, CZL-treated animals were killed at the same timing as control animals and oocytes were retrieved from the oviduct and in vitro matured for 6 h (for those gavaged with CZL, 4 h pre-hCG) or 3 h (for those gavaged with CZL, 7 h pre-hCG) to MII oocytes before IVF. These groups were designated as in vivo-in vitro synchronized/matured oocytes. In other groups treated with CZL, 4 or 7 h pre-hCG, the ovulated oocytes were allowed to mature in the oviduct (full in vivo synchronization and maturation) and oocytes were retrieved and fertilized with the same fertilization timings as the in vivo-in vitro synchronized/matured oocytes. Oocytes were cultured for 1 day after IVF and examined for cleavage. Statistical differences were analyzed by cross-tabulated chi-square test. The full in vivo synchronization and maturation (for both CZL dose timings of 4 and 7 h pre-hCG) gave significantly higher early embryonic development rates compared with those of the control [89% (n = 219) and 92.2% (n = 374) vs 81.8% (n = 198); P = 0.034 and P < 0.0001, respectively]. The in vivo-in vitro synchronized/matured oocytes (CZL dose timing at 7 h, but not 4 h pre-hCG) gave significantly higher early embryonic development rates compared with those of the control [88.5% (n = 339) vs 83.4% (n = 458), respectively; P = 0.043]. However, the increase of the IVF rate of the oocytes from mice treated with CZL, 4 h pre-hCG, in the in vivo-in vitro synchronized/matured group was not significantly different from the control group [88.5% (n = 399) vs 83.4% (n = 458), respectively; P = 0.43]. It is concluded from the present study that synchronization of oocyte meiotic maturation by the in vivo and in vivo-in-vitro protocols can increase the IVF rate of oocytes in superovulated mice.

Progestins inhibit murine oocyte meiotic maturation in vitro

1993 ◽  
Vol 265 (3) ◽  
pp. 231-239 ◽  
Author(s):  
C. Brent Barrett ◽  
R. Douglas Powers
Keyword(s):  
Meiotic Maturation ◽  
Maturation In Vitro ◽  
Oocyte Meiotic Maturation

scholarly journals In Vitro Acute Exposure to DEHP Affects Oocyte Meiotic Maturation, Energy and Oxidative Stress Parameters in a Large Animal Model

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e27452 ◽  
Author(s):  
Barbara Ambruosi ◽  
Manuel Filioli Uranio ◽  
Anna Maria Sardanelli ◽  
Paola Pocar ◽  
Nicola Antonio Martino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Animal Model ◽  
Meiotic Maturation ◽  
Large Animal Model ◽  
Large Animal ◽  
Oxidative Stress Parameters ◽  
Oocyte Meiotic Maturation ◽  
And Oxidative Stress ◽  
Stress Parameters
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