Preimplantation embryonic development of spontaneous mouse parthenotes after oocyte meiotic maturation in vitro

1981 ◽  
Vol 4 (1) ◽  
pp. 3-13 ◽  
Author(s):  
John J. Eppig
Keyword(s):  
Embryonic Development ◽  
Meiotic Maturation ◽  
Maturation In Vitro ◽  
Oocyte Meiotic Maturation

Diethylstilbestrol exposure disrupts mouse oocyte meiotic maturation in vitro through affecting spindle assembly and chromosome alignment

Chemosphere ◽  
2020 ◽  
Vol 249 ◽  
pp. 126182 ◽  
Author(s):  
Zhi-Ming Ding ◽  
Li-Ping Hua ◽  
Muhammad Jamil Ahmad ◽  
Muhammad Safdar ◽  
Fan Chen ◽  
...  
Keyword(s):  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Maturation In Vitro ◽  
Chromosome Alignment ◽  
Oocyte Meiotic Maturation

200 SYNCHRONIZATION OF OOCYTE MEIOTIC MATURATION IN SUPEROVULATED MICE IMPROVES IN VITRO FERTILIZATION RATE

2012 ◽  
Vol 24 (1) ◽  
pp. 212
Author(s):  
A. M. Taiyeb Ridha ◽  
D. C. Kraemer
Keyword(s):  
Embryonic Development ◽  
Fertilization Rate ◽  
Early Embryonic Development ◽  
Meiotic Maturation ◽  
Control Group ◽  
Vitro Fertilization ◽  
Development Rates ◽  
Oocyte Meiotic Maturation

In vitro synchronization of oocyte nuclear and cytoplasmic maturation has been found to improve the IVF rate of ovarian oocytes in several species, including humans, in comparison with nonsynchronized in vitro-matured oocytes. Here, we tested the hypothesis that synchronization of oocyte meiotic maturation by an in vivo system in superovulated mice would increase the oocyte fertilization rate when compared to that of conventional superovulated oocytes. Recently, we observed that cilostazol (CZL), a PDE3-I, was able to inhibit mouse oocyte meiotic maturation in both in vitro and in vivo systems. Administering CZL at 7.5 mg, 4 or 7 h pre-hCG allowed retrieval of ovulated oocytes of which >95% were at MI stage, scored by Nikon stereo microscope (SMZ 1500). A conventional superovulation program was adapted in all treated and their control groups, in which mice were injected with eCG and after 48 h with hCG (7.5 IU for each hormone). On the second morning, 13 to 14 h post-hCG, mice were killed and oocytes were collected from oviducts and in vitro fertilized (control). For the treated groups, CZL was administered in a single 7.5 mg oral dose (gavage) 4 or 7 h before the hCG injection. On the second morning, CZL-treated animals were killed at the same timing as control animals and oocytes were retrieved from the oviduct and in vitro matured for 6 h (for those gavaged with CZL, 4 h pre-hCG) or 3 h (for those gavaged with CZL, 7 h pre-hCG) to MII oocytes before IVF. These groups were designated as in vivo-in vitro synchronized/matured oocytes. In other groups treated with CZL, 4 or 7 h pre-hCG, the ovulated oocytes were allowed to mature in the oviduct (full in vivo synchronization and maturation) and oocytes were retrieved and fertilized with the same fertilization timings as the in vivo-in vitro synchronized/matured oocytes. Oocytes were cultured for 1 day after IVF and examined for cleavage. Statistical differences were analyzed by cross-tabulated chi-square test. The full in vivo synchronization and maturation (for both CZL dose timings of 4 and 7 h pre-hCG) gave significantly higher early embryonic development rates compared with those of the control [89% (n = 219) and 92.2% (n = 374) vs 81.8% (n = 198); P = 0.034 and P < 0.0001, respectively]. The in vivo-in vitro synchronized/matured oocytes (CZL dose timing at 7 h, but not 4 h pre-hCG) gave significantly higher early embryonic development rates compared with those of the control [88.5% (n = 339) vs 83.4% (n = 458), respectively; P = 0.043]. However, the increase of the IVF rate of the oocytes from mice treated with CZL, 4 h pre-hCG, in the in vivo-in vitro synchronized/matured group was not significantly different from the control group [88.5% (n = 399) vs 83.4% (n = 458), respectively; P = 0.43]. It is concluded from the present study that synchronization of oocyte meiotic maturation by the in vivo and in vivo-in-vitro protocols can increase the IVF rate of oocytes in superovulated mice.

Progestins inhibit murine oocyte meiotic maturation in vitro

1993 ◽  
Vol 265 (3) ◽  
pp. 231-239 ◽  
Author(s):  
C. Brent Barrett ◽  
R. Douglas Powers
Keyword(s):  
Meiotic Maturation ◽  
Maturation In Vitro ◽  
Oocyte Meiotic Maturation

Removal of Follicle Wall Components from Ovarian Oocytes of the Brook Trout, Salvelinus fontinalis

1985 ◽  
Vol 42 (12) ◽  
pp. 2053-2058 ◽  
Author(s):  
Charles A. Lessman ◽  
Soosamma Kavumpurath ◽  
Hamid R. Habibi
Keyword(s):  
Brook Trout ◽  
Salvelinus Fontinalis ◽  
Ovarian Tissue ◽  
Meiotic Maturation ◽  
Cell Surfaces ◽  
Maturation In Vitro ◽  
Free Media ◽  
Brook Trout Oocytes ◽  
Wall Components

Fully grown brook trout (Salvelinus fontinalis) ovarian follicles, preincubated in Ca2+-free media, were dissected from other ovarian tissue by use of watchmaker forceps. Manual defolliculation and further Ca2+-free treatments were used to obtain oocytes freed of one or more follicular investments. Combinations of these treatments ultimately resulted in oocytes denuded of somatic cells as demonstrated by scanning electron microscopy. Mechanically denuded brook trout oocytes maintained the ability to undergo progestogen-induced meiotic maturation in vitro. This technique, which avoids use of enzymes that may alter cell surfaces, provides a useful system for the study of interactions between follicle wall components and the oocyte during hormonally induced meiotic maturation in vitro.

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