scholarly journals Silica bioreplication preserves three-dimensional spheroid structures of human pluripotent stem cells and HepG2 cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yan-Ru Lou ◽  
Liisa Kanninen ◽  
Bryan Kaehr ◽  
Jason L. Townson ◽  
Johanna Niklander ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Hepg2 Cells ◽  
Three Dimensional ◽  
Human Pluripotent Stem Cells

scholarly journals C-STEM: ENGINEERING NICHE-LIKE MICRO-COMPARTMENTS FOR OPTIMAL AND SCALE-INDEPENDENT EXPANSION OF HUMAN PLURIPOTENT STEM CELLS IN BIOREACTORS

2021 ◽  
Author(s):  
Philippe J.R. Cohen ◽  
Elisa Luquet ◽  
Justine Pletenka ◽  
Andrea Leonard ◽  
Elise Warter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Large Scale ◽  
Scale Up ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Human Pluripotent Stem Cells ◽  
Cell Therapies ◽  
Cellular Source

Human pluripotent stem cells (hPSCs) have emerged as the most promising cellular source for cell therapies. To overcome scale up limitations of classical 2D culture systems, suspension cultures have been developed to meet the need of large-scale culture in regenerative medicine. Despite constant improvements, current protocols relying on the generation of micro-carriers or cell aggregates only achieve moderate amplification performance. Here, guided by reports showing that hPSCs can self-organize in vitro into cysts reminiscent of the epiblast stage in embryo development, we developed a physio-mimetic approach for hPSC culture. We engineered stem cell niche microenvironments inside microfluidics-assisted core-shell microcapsules. We demonstrate that lumenized three-dimensional colonies maximize viability and expansion rates while maintaining pluripotency. By optimizing capsule size and culture conditions, we scale-up this method to industrial scale stirred tank bioreactors and achieve an unprecedented hPSC amplification rate of 282-fold in 6.5 days.

scholarly journals Three-dimensional induction of dorsal, intermediate and ventral spinal cord tissues from human pluripotent stem cells

Development ◽  
2018 ◽  
Vol 145 (16) ◽  
pp. dev162214 ◽  
Author(s):  
Takenori Ogura ◽  
Hideya Sakaguchi ◽  
Susumu Miyamoto ◽  
Jun Takahashi
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Human Pluripotent Stem Cells ◽  
Ventral Spinal Cord

scholarly journals Three-dimensional cardiac microtissues composed of cardiomyocytes and endothelial cells co-differentiated from human pluripotent stem cells

Development ◽  
2017 ◽  
Vol 144 (6) ◽  
pp. 1008-1017 ◽  
Author(s):  
Elisa Giacomelli ◽  
Milena Bellin ◽  
Luca Sala ◽  
Berend J. van Meer ◽  
Leon G. J. Tertoolen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Human Pluripotent Stem Cells
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