scholarly journals Three-dimensional cage-like microscaffolds for cell invasion studies

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Barbara Spagnolo ◽  
Virgilio Brunetti ◽  
Godefroy Leménager ◽  
Elisa De Luca ◽  
Leonardo Sileo ◽  
...  
Keyword(s):  
Cell Invasion ◽  
Three Dimensional

scholarly journals α5β1-Integrin promotes tension-dependent mammary epithelial cell invasion by engaging the fibronectin synergy site

2017 ◽  
Vol 28 (22) ◽  
pp. 2958-2977 ◽  
Author(s):  
Y. A. Miroshnikova ◽  
G. I. Rozenberg ◽  
L. Cassereau ◽  
M. Pickup ◽  
J. K. Mouw ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Malignant Transformation ◽  
Cell Invasion ◽  
Adhesion Force ◽  
Adhesion Formation ◽  
Three Dimensional ◽  
Growth Factor Receptor ◽  
Mammary Epithelial ◽  
Α5β1 Integrin

Tumors are fibrotic and characterized by abundant, remodeled, and cross-linked collagen that stiffens the extracellular matrix stroma. The stiffened collagenous stroma fosters malignant transformation of the tissue by increasing tumor cell tension to promote focal adhesion formation and potentiate growth factor receptor signaling through kinase. Importantly, collagen cross-linking requires fibronectin (FN). Fibrotic tumors contain abundant FN, and tumor cells frequently up-regulate the FN receptor α5β1 integrin. Using transgenic and xenograft models and tunable two- and three-dimensional substrates, we show that FN-bound α5β1 integrin promotes tension-dependent malignant transformation through engagement of the synergy site that enhances integrin adhesion force. We determined that ligation of the synergy site of FN permits tumor cells to engage a zyxin-stabilized, vinculin-linked scaffold that facilitates nucleation of phosphatidylinositol (3,4,5)-triphosphate at the plasma membrane to enhance phosphoinositide 3-kinase (PI3K)-dependent tumor cell invasion. The data explain why rigid collagen fibrils potentiate PI3K activation to promote malignancy and offer a perspective regarding the consistent up-regulation of α5β1 integrin and FN in many tumors and their correlation with cancer aggression.

Redox-Directed Suppression of Malignant Cell Invasion in a Three-Dimensional Skin Reconstruct Model of Metastatic Melanoma

2014 ◽  
Vol 76 ◽  
pp. S80
Author(s):  
Angela L. Davis ◽  
Shuxi Qiao ◽  
Rebecca Justiniano ◽  
Montserrat Rojo de la Vega ◽  
Georg T. Wondrak
Keyword(s):  
Metastatic Melanoma ◽  
Cell Invasion ◽  
Three Dimensional ◽  
Malignant Cell

Micropassage-embedding composite hydrogel fibers enable quantitative evaluation of cancer cell invasion under 3D coculture conditions

Lab on a Chip ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 1378-1387 ◽  
Author(s):  
Manami Sugimoto ◽  
Yoichi Kitagawa ◽  
Masumi Yamada ◽  
Yuya Yajima ◽  
Rie Utoh ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Quantitative Evaluation ◽  
Cell Invasion ◽  
Three Dimensional ◽  
Composite Hydrogel ◽  
Cancer Cell Invasion ◽  
New System

A new system for quantitatively evaluating cancer cell invasion in a three-dimensional environment was developed using composite hydrogel microfibers having a micropassage.

Digital microfluidics for spheroid-based invasion assays

Lab on a Chip ◽  
2016 ◽  
Vol 16 (8) ◽  
pp. 1505-1513 ◽  
Author(s):  
Brian F. Bender ◽  
Andrew. P. Aijian ◽  
Robin. L. Garrell
Keyword(s):  
Drug Discovery ◽  
Cell Invasion ◽  
Cell Biology ◽  
Digital Microfluidics ◽  
Three Dimensional ◽  
Multicellular Spheroids ◽  
Microfluidic Platform ◽  
Digital Microfluidic ◽  
Biology Research ◽  
Invasion Assays

A digital microfluidic platform that enables the formation, gel encapsulation, and assaying of three-dimensional multicellular spheroids is described. Such a platform can facilitate automation of cell invasion assays for cell biology research and drug discovery.

scholarly journals Three-dimensional cultured tissue constructs that imitate human living tissue organization for analysis of tumor cell invasion

2018 ◽  
Vol 107 (2) ◽  
pp. 292-300 ◽  
Author(s):  
Soichi Iwai ◽  
Satoko Kishimoto ◽  
Yuto Amano ◽  
Akihiro Nishiguchi ◽  
Michiya Matsusaki ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Invasion ◽  
Three Dimensional ◽  
Tumor Cell Invasion ◽  
Living Tissue ◽  
Tissue Constructs ◽  
Tissue Organization

scholarly journals Extracellular Matrix Heterogeneity Regulates Three-Dimensional Morphologies of Breast Adenocarcinoma Cell Invasion

2012 ◽  
Vol 2 (6) ◽  
pp. 790-794 ◽  
Author(s):  
Yoojin Shin ◽  
Hyunju Kim ◽  
Sewoon Han ◽  
Jihee Won ◽  
Hyo Eun Jeong ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Invasion ◽  
Three Dimensional ◽  
Breast Adenocarcinoma ◽  
Adenocarcinoma Cell ◽  
Matrix Heterogeneity
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