scholarly journals Triggering mitophagy with far-red fluorescent photosensitizers

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Cheng-Wei Hsieh ◽  
Chih-Hang Chu ◽  
Hsien-Ming Lee ◽  
Wei Yuan Yang
Keyword(s):  
Cell Death ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Mitochondrial Damage ◽  
Cellular Mechanism ◽  
Live Cell ◽  
Temporal Precision ◽  
Highly Correlated

Abstract Cells identify defective mitochondria and eliminate them through mitophagy: this allows cells to rid themselves of unwanted stress to maintain health and avoid the activation of cell death. One approach to experimentally investigate mitophagy is through the use of mitochondrial photosensitizers, which when coupled with light allows one to precisely control mitochondrial damage with spatial and temporal precision. Here we report three far-red fluorophores that can be used as robust mitochondrial photosensitizers to initiate mitophagy. The dyes offer maximal compatibility with multi-color live-cell imaging, as they do not spectrally overlap with commonly used fluorescent proteins. Through the use of these far-red fluorescent photosensitizers we found that mitophagic engulfment and mitophagosome maturation rates are highly correlated with the cellular Parkin-labeled mitochondria levels. This may represent a protective cellular mechanism to avoid membrane and lysosome depletion during mitophagy.

scholarly journals A single promoter system co-expressing RNA sensor with fluorescent proteins for quantitative mRNA imaging in living tumor cells

2019 ◽  
Vol 10 (18) ◽  
pp. 4828-4833 ◽  
Author(s):  
Zhan-Ming Ying ◽  
Yue-Yan Yuan ◽  
Bin Tu ◽  
Li-Juan Tang ◽  
Ru-Qin Yu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Live Cell ◽  
Rna Aptamers ◽  
Rna Sensors ◽  
Promoter System ◽  
Single Promoter

Genetically encoded light-up RNA aptamers afford a valuable platform for developing RNA sensors toward live cell imaging.

scholarly journals tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

2021 ◽  
Author(s):  
Y. Bousmah ◽  
H. Valenta ◽  
G. Bertolin ◽  
U. Singh ◽  
V. Nicolas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy ◽  
Live Cell ◽  
Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

scholarly journals Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

IUBMB Life ◽  
2009 ◽  
Vol 61 (11) ◽  
pp. 1029-1042 ◽  
Author(s):  
Jörg Wiedenmann ◽  
Franz Oswald ◽  
Gerd Ulrich Nienhaus
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Live Cell

Utility of Cytoplasmic Fluorescent Proteins for Live-Cell Imaging of Magnaporthe grisea in Planta

Mycologia ◽  
2002 ◽  
Vol 94 (2) ◽  
pp. 280 ◽  
Author(s):  
Kirk J. Czymmek ◽  
Timothy M. Bourett ◽  
James A. Sweigard ◽  
Anne Carroll ◽  
Richard J. Howard
Keyword(s):  
Live Cell Imaging ◽  
Magnaporthe Grisea ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Live Cell ◽  
In Planta

scholarly journals Long-term fate of etoposide-induced micronuclei and micronucleated cells in Hela-H2B-GFP cells

2020 ◽  
Vol 94 (10) ◽  
pp. 3553-3561
Author(s):  
Hauke Reimann ◽  
Helga Stopper ◽  
Henning Hintzsche
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cellular Structures ◽  
Death Rates ◽  
Daughter Cells

Abstract Micronuclei are small nuclear cellular structures containing whole chromosomes or chromosomal fragments. While there is a lot of information available about the origin and formation of micronuclei, less is known about the fate of micronuclei and micronucleated cells. Possible fates include extrusion, degradation, reincorporation and persistence. Live cell imaging was performed to quantitatively analyse the fates of micronuclei and micronucleated cells occurring in vitro. Imaging was conducted for up to 96 h in HeLa-H2B-GFP cells treated with 0.5, 1 and 2 µg/ml etoposide. While a minority of micronuclei was reincorporated into the main nucleus during mitosis, the majority of micronuclei persisted without any alterations. Degradation and extrusion were observed rarely or never. The presence of micronuclei affected the proliferation of the daughter cells and also had an influence on cell death rates. Mitotic errors were found to be clearly increased in micronucleus-containing cells. The results show that micronuclei and micronucleated cells can, although delayed in cell cycle, sustain for multiple divisions.

Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging

2013 ◽  
Vol 9 (2) ◽  
pp. 258-267 ◽  
Author(s):  
Marie Erard ◽  
Asma Fredj ◽  
Hélène Pasquier ◽  
Dahdjim-Benoît Beltolngar ◽  
Yasmina Bousmah ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Live Cell
Sign in / Sign up

Export Citation Format

Share Document