scholarly journals Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

IUBMB Life ◽  
2009 ◽  
Vol 61 (11) ◽  
pp. 1029-1042 ◽  
Author(s):  
Jörg Wiedenmann ◽  
Franz Oswald ◽  
Gerd Ulrich Nienhaus
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Live Cell

scholarly journals A single promoter system co-expressing RNA sensor with fluorescent proteins for quantitative mRNA imaging in living tumor cells

2019 ◽  
Vol 10 (18) ◽  
pp. 4828-4833 ◽  
Author(s):  
Zhan-Ming Ying ◽  
Yue-Yan Yuan ◽  
Bin Tu ◽  
Li-Juan Tang ◽  
Ru-Qin Yu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Live Cell ◽  
Rna Aptamers ◽  
Rna Sensors ◽  
Promoter System ◽  
Single Promoter

Genetically encoded light-up RNA aptamers afford a valuable platform for developing RNA sensors toward live cell imaging.

scholarly journals tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

2021 ◽  
Author(s):  
Y. Bousmah ◽  
H. Valenta ◽  
G. Bertolin ◽  
U. Singh ◽  
V. Nicolas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy ◽  
Live Cell ◽  
Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

Utility of Cytoplasmic Fluorescent Proteins for Live-Cell Imaging of Magnaporthe grisea in Planta

Mycologia ◽  
2002 ◽  
Vol 94 (2) ◽  
pp. 280 ◽  
Author(s):  
Kirk J. Czymmek ◽  
Timothy M. Bourett ◽  
James A. Sweigard ◽  
Anne Carroll ◽  
Richard J. Howard
Keyword(s):  
Live Cell Imaging ◽  
Magnaporthe Grisea ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Live Cell ◽  
In Planta

Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging

2013 ◽  
Vol 9 (2) ◽  
pp. 258-267 ◽  
Author(s):  
Marie Erard ◽  
Asma Fredj ◽  
Hélène Pasquier ◽  
Dahdjim-Benoît Beltolngar ◽  
Yasmina Bousmah ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Live Cell

scholarly journals Triggering mitophagy with far-red fluorescent photosensitizers

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Cheng-Wei Hsieh ◽  
Chih-Hang Chu ◽  
Hsien-Ming Lee ◽  
Wei Yuan Yang
Keyword(s):  
Cell Death ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Mitochondrial Damage ◽  
Cellular Mechanism ◽  
Live Cell ◽  
Temporal Precision ◽  
Highly Correlated

Abstract Cells identify defective mitochondria and eliminate them through mitophagy: this allows cells to rid themselves of unwanted stress to maintain health and avoid the activation of cell death. One approach to experimentally investigate mitophagy is through the use of mitochondrial photosensitizers, which when coupled with light allows one to precisely control mitochondrial damage with spatial and temporal precision. Here we report three far-red fluorophores that can be used as robust mitochondrial photosensitizers to initiate mitophagy. The dyes offer maximal compatibility with multi-color live-cell imaging, as they do not spectrally overlap with commonly used fluorescent proteins. Through the use of these far-red fluorescent photosensitizers we found that mitophagic engulfment and mitophagosome maturation rates are highly correlated with the cellular Parkin-labeled mitochondria levels. This may represent a protective cellular mechanism to avoid membrane and lysosome depletion during mitophagy.

scholarly journals Collection of Recombinant Rotaviruses Expressing Fluorescent Reporter Proteins

2019 ◽  
Vol 8 (27) ◽  
Author(s):  
Asha A. Philip ◽  
Brittany E. Herrin ◽  
Maximiliano L. Garcia ◽  
Andrew T. Abad ◽  
Sarah P. Katen ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Genome Segment ◽  
Live Cell ◽  
Fluorescent Reporter ◽  
Fluorescent Markers ◽  
Reporter Proteins

ABSTRACT A collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for the fluorescent proteins were inserted into genome segment 7 without compromising expression of the protein NSP3. These recombinant rotaviruses are valuable for analyzing rotavirus biology by fluorescence-based live-cell imaging.

Fluorescent proteins for live-cell imaging with super-resolution

2014 ◽  
Vol 43 (4) ◽  
pp. 1088-1106 ◽  
Author(s):  
Karin Nienhaus ◽  
G. Ulrich Nienhaus
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Proteins ◽  
Super Resolution ◽  
Live Cell
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