scholarly journals Towards an in vitro model mimicking the foreign body response: tailoring the surface properties of biomaterials to modulate extracellular matrix

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Febriyani F. R. Damanik ◽  
Tonia C. Rothuizen ◽  
Clemens van Blitterswijk ◽  
Joris I. Rotmans ◽  
Lorenzo Moroni
Keyword(s):  
Extracellular Matrix ◽  
Foreign Body ◽  
Surface Properties ◽  
In Vitro Model ◽  
Foreign Body Response ◽  
Vitro Model ◽  
Body Response

Amniotic Membrane as an in vitro Model for Endometrium- Extracellular Matrix Interactions

1998 ◽  
Vol 45 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Paul J.Q. van der Linden ◽  
Anton F.P.M. de Goeij ◽  
Gerard A.J. Dunselman ◽  
Heleen W.H. Erkens ◽  
Johannes L.H. Evers
Keyword(s):  
Extracellular Matrix ◽  
Amniotic Membrane ◽  
In Vitro Model ◽  
Matrix Interactions ◽  
Vitro Model ◽  
Extracellular Matrix Interactions

scholarly journals Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins

Nephron ◽  
2019 ◽  
Vol 142 (4) ◽  
pp. 328-350 ◽  
Author(s):  
Hélène Bon ◽  
Paul Hales ◽  
Simon Lumb ◽  
Gill Holdsworth ◽  
Tim Johnson ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Renal Fibrosis ◽  
In Vitro Model ◽  
Vitro Model ◽  
Matrix Accumulation

scholarly journals In Vitro Model of Metastasis to Bone Marrow Mediates Prostate Cancer Castration Resistant Growth through Paracrine and Extracellular Matrix Factors

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e40372 ◽  
Author(s):  
Reynald M. Lescarbeau ◽  
F. Philipp Seib ◽  
Marina Prewitz ◽  
Carsten Werner ◽  
David L. Kaplan
Keyword(s):  
Prostate Cancer ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
In Vitro Model ◽  
Castration Resistant ◽  
Vitro Model ◽  
Matrix Factors

Immunolocalisation of collagenase in rabbit periosteal tissue explants and extraction of the enzyme. The effect of the cytokines IL-1 alpha and EGF

1994 ◽  
Vol 107 (4) ◽  
pp. 1047-1053 ◽  
Author(s):  
E. van der Zee ◽  
V. Everts ◽  
K. Hoeben ◽  
W. Beertsen
Keyword(s):  
Extracellular Matrix ◽  
In Vitro Model ◽  
Interleukin 1 ◽  
Immunohistochemical Analysis ◽  
Model System ◽  
High Concentration ◽  
Active Collagenase ◽  
Epidermal Growth ◽  
Vitro Model

The effect of interleukin-1 alpha (IL-1 alpha) and murine epidermal growth factor (EGF) on incorporation of endogenously produced collagenase in the extracellular matrix of soft connective tissue was studied in an in vitro model system using periosteal explants obtained from rabbit calvariae. Immunohistochemical analysis indicated the highest level of collagenase in explants cultured for 72 hours with IL-1 alpha in combination with EGF. Most enzyme appeared to be associated with the extracellular matrix, but labeling was also found in numerous fibroblast-like cells. Explants cultured in the presence of IL-1 alpha alone contained less enzyme and in periostea treated without cytokines, or with EGF alone, only a faint label, if any, was seen. Freshly isolated, non-cultured periostea contained no detectable enzyme. Extraction of collagenase from periostea revealed that: (1) non-cultured periosteum did not contain detectable levels of enzyme. (2) The amount of total activatable enzyme synergistically increased (10-fold) under the influence of IL-1 alpha and EGF, whereas IL-1 alpha alone showed a 4-fold enhancement compared to control or EGF-incubated explants. (3) The latent fraction of the enzyme was synergistically increased (up to 100-fold or more) in periostea cultured in the presence of IL-1 alpha + EGF (21.17 mU/explant versus 0.05 mU/explant in controls). (4) Active collagenase, on the other hand, appeared to be present in a relatively high concentration in explants cultured without cytokines (2.45 mU/explant versus 0.36 mU/explant in IL-1 alpha + EGF-treated explants).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Fourier transform infrared spectroscopy to quantify collagen and elastin in an in vitro model of extracellular matrix degradation in aorta

The Analyst ◽  
2014 ◽  
Vol 139 (12) ◽  
pp. 3039-3047 ◽  
Author(s):  
Rabee Cheheltani ◽  
Cushla M. McGoverin ◽  
Jayashree Rao ◽  
David A. Vorp ◽  
Mohammad F. Kiani ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
In Vitro Model ◽  
Fourier Transform Infrared ◽  
Matrix Degradation ◽  
Extracellular Matrix Degradation ◽  
Vitro Model

FTIR-based analyses can quantify elastin and collagen in vascular tissues.

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