Amniotic Membrane as an in vitro Model for Endometrium- Extracellular Matrix Interactions

1998 ◽  
Vol 45 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Paul J.Q. van der Linden ◽  
Anton F.P.M. de Goeij ◽  
Gerard A.J. Dunselman ◽  
Heleen W.H. Erkens ◽  
Johannes L.H. Evers
Keyword(s):  
Extracellular Matrix ◽  
Amniotic Membrane ◽  
In Vitro Model ◽  
Matrix Interactions ◽  
Vitro Model ◽  
Extracellular Matrix Interactions

A rhabdomyosarcoma hydrogel model to unveil cell-extracellular matrix interactions

2021 ◽  
Author(s):  
Mattia Saggioro ◽  
Stefania D'Agostino ◽  
Anna Gallo ◽  
Sara Crotti ◽  
Sara D'Aronco ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
3D Culture ◽  
3D Cell Culture ◽  
Culture Systems ◽  
Matrix Interactions ◽  
In Vitro Systems ◽  
Extracellular Matrix Interactions

Three-dimensional (3D) culture systems are progressively getting attention given their potential in overcoming limitations of the classical 2D in vitro systems. Among different supports for 3D cell culture, hydrogels (HGs)...

scholarly journals Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application

2020 ◽  
Vol 21 (17) ◽  
pp. 6225 ◽  
Author(s):  
Kamila Białkowska ◽  
Piotr Komorowski ◽  
Maria Bryszewska ◽  
Katarzyna Miłowska
Keyword(s):  
Cell Cultures ◽  
Cell Biology ◽  
Disease Modeling ◽  
Three Dimensional ◽  
3D Culture ◽  
Matrix Interactions ◽  
Vitro Model ◽  
Extracellular Matrix Interactions ◽  
Natural Cell

Cell cultures are very important for testing materials and drugs, and in the examination of cell biology and special cell mechanisms. The most popular models of cell culture are two-dimensional (2D) as monolayers, but this does not mimic the natural cell environment. Cells are mostly deprived of cell–cell and cell–extracellular matrix interactions. A much better in vitro model is three-dimensional (3D) culture. Because many cell lines have the ability to self-assemble, one 3D culturing method is to produce spheroids. There are several systems for culturing cells in spheroids, e.g., hanging drop, scaffolds and hydrogels, and these cultures have their applications in drug and nanoparticles testing, and disease modeling. In this paper we would like to present methods of preparation of spheroids in general and emphasize the most important applications.

scholarly journals Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins

Nephron ◽  
2019 ◽  
Vol 142 (4) ◽  
pp. 328-350 ◽  
Author(s):  
Hélène Bon ◽  
Paul Hales ◽  
Simon Lumb ◽  
Gill Holdsworth ◽  
Tim Johnson ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Renal Fibrosis ◽  
In Vitro Model ◽  
Vitro Model ◽  
Matrix Accumulation

scholarly journals In Vitro Model of Metastasis to Bone Marrow Mediates Prostate Cancer Castration Resistant Growth through Paracrine and Extracellular Matrix Factors

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e40372 ◽  
Author(s):  
Reynald M. Lescarbeau ◽  
F. Philipp Seib ◽  
Marina Prewitz ◽  
Carsten Werner ◽  
David L. Kaplan
Keyword(s):  
Prostate Cancer ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
In Vitro Model ◽  
Castration Resistant ◽  
Vitro Model ◽  
Matrix Factors

Immunolocalisation of collagenase in rabbit periosteal tissue explants and extraction of the enzyme. The effect of the cytokines IL-1 alpha and EGF

1994 ◽  
Vol 107 (4) ◽  
pp. 1047-1053 ◽  
Author(s):  
E. van der Zee ◽  
V. Everts ◽  
K. Hoeben ◽  
W. Beertsen
Keyword(s):  
Extracellular Matrix ◽  
In Vitro Model ◽  
Interleukin 1 ◽  
Immunohistochemical Analysis ◽  
Model System ◽  
High Concentration ◽  
Active Collagenase ◽  
Epidermal Growth ◽  
Vitro Model

The effect of interleukin-1 alpha (IL-1 alpha) and murine epidermal growth factor (EGF) on incorporation of endogenously produced collagenase in the extracellular matrix of soft connective tissue was studied in an in vitro model system using periosteal explants obtained from rabbit calvariae. Immunohistochemical analysis indicated the highest level of collagenase in explants cultured for 72 hours with IL-1 alpha in combination with EGF. Most enzyme appeared to be associated with the extracellular matrix, but labeling was also found in numerous fibroblast-like cells. Explants cultured in the presence of IL-1 alpha alone contained less enzyme and in periostea treated without cytokines, or with EGF alone, only a faint label, if any, was seen. Freshly isolated, non-cultured periostea contained no detectable enzyme. Extraction of collagenase from periostea revealed that: (1) non-cultured periosteum did not contain detectable levels of enzyme. (2) The amount of total activatable enzyme synergistically increased (10-fold) under the influence of IL-1 alpha and EGF, whereas IL-1 alpha alone showed a 4-fold enhancement compared to control or EGF-incubated explants. (3) The latent fraction of the enzyme was synergistically increased (up to 100-fold or more) in periostea cultured in the presence of IL-1 alpha + EGF (21.17 mU/explant versus 0.05 mU/explant in controls). (4) Active collagenase, on the other hand, appeared to be present in a relatively high concentration in explants cultured without cytokines (2.45 mU/explant versus 0.36 mU/explant in IL-1 alpha + EGF-treated explants).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Fourier transform infrared spectroscopy to quantify collagen and elastin in an in vitro model of extracellular matrix degradation in aorta

The Analyst ◽  
2014 ◽  
Vol 139 (12) ◽  
pp. 3039-3047 ◽  
Author(s):  
Rabee Cheheltani ◽  
Cushla M. McGoverin ◽  
Jayashree Rao ◽  
David A. Vorp ◽  
Mohammad F. Kiani ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
In Vitro Model ◽  
Fourier Transform Infrared ◽  
Matrix Degradation ◽  
Extracellular Matrix Degradation ◽  
Vitro Model

FTIR-based analyses can quantify elastin and collagen in vascular tissues.

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