scholarly journals Rapid generation of functional mature pancreatic islet-beta cells from human pluripotent stem cells

2019 ◽  
Author(s):  
Gopika Nair ◽  
Gopika Nair ◽  
Holger Russ ◽  
Matthias Hebrok
Keyword(s):  
Stem Cells ◽  
Beta Cells ◽  
Pancreatic Islet ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Islet Beta Cells ◽  
Pancreatic Islet Beta Cells ◽  
Rapid Generation

scholarly journals Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cells

2021 ◽  
Author(s):  
Rabea Dettmer ◽  
Isabell Niwolik ◽  
Ilir Mehmeti ◽  
Anne Jörns ◽  
Ortwin Naujok
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Beta Cells ◽  
Pluripotent Stem Cells ◽  
Reporter Genes ◽  
Human Pluripotent Stem Cells ◽  
Endogenous Gene ◽  
Pancreatic Progenitors ◽  
Insulin Producing Cells ◽  
Endogenous Genes

AbstractDifferentiation of human pluripotent stem cells into insulin-producing stem cell-derived beta cells harbors great potential for research and therapy of diabetes. The SOX9 gene plays a crucial role during development of the pancreas and particularly in the development of insulin-producing cells as SOX9+ cells form the source for NEUROG3+ endocrine progenitor cells. For the purpose of easy monitoring of differentiation efficiencies into pancreatic progenitors and insulin-producing cells, we generated new reporter lines by knocking in a P2A-H-2Kk-F2A-GFP2 reporter genes into the SOX9 locus and a P2A-mCherry reporter gene into the INS locus mediated by CRISPR/CAS9-technology. The knock-ins enable co-expression of the endogenous genes and reporter genes, report the endogenous gene expression and enable the purification of pancreatic progenitors and insulin-producing cells using FACS or MACS. Using these cell lines we established a new differentiation protocol geared towards SOX9+ cells to efficiently drive human pluripotent stem cells into glucose-responsive beta cells.

Selective expression of an arachidonate 12-lipoxygenase by pancreatic islet beta-cells

1992 ◽  
Vol 263 (5) ◽  
pp. E828-E836 ◽  
Author(s):  
V. R. Shannon ◽  
S. Ramanadham ◽  
J. Turk ◽  
M. J. Holtzman
Keyword(s):  
Beta Cell ◽  
Enzymatic Activity ◽  
Beta Cells ◽  
Pancreatic Islet ◽  
Cell Populations ◽  
Intense Staining ◽  
Rat Pancreas ◽  
Islet Beta Cells ◽  
Pancreatic Islet Beta Cells ◽  
12 Lipoxygenase

The immunohistochemical distribution of arachidonate lipoxygenases in rat pancreas was characterized with specific polyclonal anti-5-lipoxygenase and anti-12-lipoxygenase antibodies. Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded rat pancreas using anti-12-lipoxygenase antibody and biotin-avidin-peroxidase detection demonstrated specific staining of islets and no staining of pancreatic exocrine tissue. Less intense staining of pancreatic vascular myocytes and endothelial cells was also observed. Immunoblotting of isolated pancreatic islet extracts with the anti-12-lipoxygenase antibody demonstrated immunoperoxidase staining of a single protein band which comigrated with purified 12-lipoxygenase (relative molecular weight = 72,000) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Dispersed cells prepared from isolated islets and then subjected to fluorescence-activated cell sorting and immunostaining exhibited 12-lipoxygenase antigen in beta-cell populations but not in non-beta-cell (predominantly alpha-cell) populations. Assays of enzymatic activity confirmed that the 12-lipoxygenase-catalyzed conversion of arachidonic acid to 12-hydroxyeicosatetraenoic acid methyl ester occurred only with purified beta-cells and not with islet non-beta-cells. No evidence of 5-lipoxygenase antigen or enzymatic activity was found in purified beta-cells or in islet non-beta-cells. We conclude that rat pancreatic islet beta-cells contain an arachidonate 12-lipoxygenase which shares antigenic epitopes with the homologous enzyme contained in tissues from other species. In addition, the selective localization of the 12-lipoxygenase to pancreatic beta-cells and its absence in pancreatic acinar cells and in islet non-beta-cells support observations suggesting that 12-lipoxygenase products may participate in glucose-induced insulin secretion from beta-cells.

scholarly journals Role of caspases in the regulation of apoptotic pancreatic islet beta-cells death

2004 ◽  
Vol 200 (2) ◽  
pp. 177-200 ◽  
Author(s):  
Hongxiang Hui ◽  
Francesco Dotta ◽  
Umberto Di Mario ◽  
Riccardo Perfetti
Keyword(s):  
Beta Cells ◽  
Pancreatic Islet ◽  
Islet Beta Cells ◽  
Pancreatic Islet Beta Cells ◽  
Cells Death

scholarly journals In vivo proliferation of differentiated pancreatic islet beta cells in transgenic mice expressing mutated cyclin-dependent kinase 4

Diabetologia ◽  
2004 ◽  
Vol 47 (10) ◽  
pp. 1819-1830 ◽  
Author(s):  
S. Hino ◽  
T. Yamaoka ◽  
Y. Yamashita ◽  
T. Yamada ◽  
J. Hata ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Beta Cells ◽  
Pancreatic Islet ◽  
Cyclin Dependent Kinase ◽  
Islet Beta Cells ◽  
Pancreatic Islet Beta Cells
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