Isolation of exosomal RNA from cell culture supernatant using the System Biosciences Exoquick Seramir tissue culture kit

2017 ◽  
Author(s):  
Roger P. Alexander ◽  
Kirsty Danielson ◽  
Bridget Simonson ◽  
Srimeenakshi Srinivasan ◽  
Irene K. Yan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tissue Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant

Presence of Trypanosoma cruzi antigen on the surface of both infected and uninfected cells in tissue culture

Parasitology ◽  
1980 ◽  
Vol 80 (1) ◽  
pp. 147-152 ◽  
Author(s):  
I. A. Abrahamsohn ◽  
J. K. Kloetzel
Keyword(s):  
Cell Culture ◽  
Tissue Culture ◽  
Trypanosoma Cruzi ◽  
Culture Medium ◽  
Culture Supernatant ◽  
Uninfected Cell ◽  
Cell Culture Supernatant ◽  
Supernatant Fluid ◽  
Cell Monolayers ◽  
Infected Cells

SummaryLLC-MK2 cell monolayers infected with Trypanosoma cruzi were shown by immunofluorescence to present parasite antigens on the surface of both parasitized and non-parasitized cells after completion of the first intracellular cycle and rupture of infected cells. The cell-culture supernatant fluid at this stage, as well as the supernatant fluid of parasites left overnight in culture medium were concentrated and contained antigen capable of binding to uninfected cell monolayers. The origin of this antigen, as well as its eventual role in the pathogenesis of Chagas' disease, are discussed.

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

2013 ◽  
Vol 8 (3) ◽  
pp. 352-362 ◽  
Author(s):  
Paula A. J. Rosa ◽  
Ana M. Azevedo ◽  
S. Sommerfeld ◽  
Martina Mutter ◽  
Werner Bäcker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Precipitation of VEGF from mesenchymal stem cell culture supernatant using the PCA process

2019 ◽  
Vol 151 ◽  
pp. 40-48 ◽  
Author(s):  
Hee Jeong Park ◽  
Tae Jun Yoon ◽  
Won-Su Son ◽  
Chan-Ju Lee ◽  
Si-na Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Mesenchymal Stem Cell Culture ◽  
Stem Cell Culture

scholarly journals Factors influencing the measurement of the secretion rate of extracellular vesicles

The Analyst ◽  
2020 ◽  
Vol 145 (17) ◽  
pp. 5870-5877
Author(s):  
Yi Wen ◽  
Yundi Chen ◽  
Guosheng Wang ◽  
Komal Abhange ◽  
Fei Xue ◽  
...  
Keyword(s):  
Cell Culture ◽  
Direct Measurement ◽  
Extracellular Vesicles ◽  
Culture Supernatant ◽  
Extracellular Vesicle ◽  
Secretion Rate ◽  
Cell Culture Supernatant ◽  
Factors Influencing

Direct measurement of the small extracellular vesicle in the cell culture supernatant.

scholarly journals Design and Manufacturing of Electromechanical Chip for Rapid Measurement of IgG1 Antibody in Cell-Culture Supernatant

2016 ◽  
Vol 08 (07) ◽  
Author(s):  
Azam Rahimpour ◽  
Fatemeh Yazdian ◽  
Zahra Akbari Jonoush ◽  
Masoumeh Rajabibazl ◽  
Meisam Omi
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Rapid Measurement ◽  
Igg1 Antibody ◽  
Design And Manufacturing
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