scholarly journals Factors influencing the measurement of the secretion rate of extracellular vesicles

The Analyst ◽  
2020 ◽  
Vol 145 (17) ◽  
pp. 5870-5877
Author(s):  
Yi Wen ◽  
Yundi Chen ◽  
Guosheng Wang ◽  
Komal Abhange ◽  
Fei Xue ◽  
...  
Keyword(s):  
Cell Culture ◽  
Direct Measurement ◽  
Extracellular Vesicles ◽  
Culture Supernatant ◽  
Extracellular Vesicle ◽  
Secretion Rate ◽  
Cell Culture Supernatant ◽  
Factors Influencing

Direct measurement of the small extracellular vesicle in the cell culture supernatant.

scholarly journals Isolation of small extracellular vesicles from cell culture supernatant

2020 ◽  
Author(s):  
Eun-Ju Im ◽  
Chan-Hyeong Lee ◽  
Moon-Chang Baek
Keyword(s):  
Cell Culture ◽  
Extracellular Vesicles ◽  
Culture Supernatant ◽  
Culture Media ◽  
Cell Culture Supernatant ◽  
Cell Culture Media

Abstract This protocol describes how to isolate small extracellular vesicles (sEV) from cell culture media using an ultracentrifuge and analyze the number of sEV by nanoparticle tracking analyzer.

Separation of Extracellular Vesicles by DNA-Directed Immunocapturing Followed by Enzymatic Release

2020 ◽  
Author(s):  
Dario Brambilla ◽  
Laura Sola ◽  
Elisa Chiodi ◽  
Natasa Zarovni ◽  
Diogo Fortunato ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Vesicles ◽  
Culture Media ◽  
Biological Fluids ◽  
Imaging Techniques ◽  
Cell Communication ◽  
Disease Diagnosis ◽  
Cell Culture Supernatant ◽  
Transmission Electron ◽  
Downstream Analysis

Extracellular vesicles (EVs) have attracted great interest among researchers due to their role in cell-cell communication, disease diagnosis, and drug delivery. In spite of their potential in the medical field, there is no consensus on the best method for separating microvesicles from cell culture supernatant and complex biological fluids. Obtaining a good recovery yield and preserving physical characteristics is critical for the diagnostic and therapeutic use of EVs. The separation is made complex by the fact that blood and cell culture media, contain a large number of nanoparticles in the same size range. Methods that exploit immunoaffinity capture provide high purity samples and overcome the issues of currently used separation methods. However, the release of captured nanovesicles requires harsh conditions that hinder their use in certain types of downstream analysis. Herein, a novel capture and release approach for small extracellular vesicles (sEVs), based on DNAdirected immobilization of antiCD63 antibody is presented. The flexible DNAlinker increases the capture efficiency and allows releasing of EVs by exploiting the endonucleasic activity of DNAse I. This separation protocol works under mild conditions, enabling the release of intact vesicles that can be successfully analyzed by imaging techniques. In this article sEVs recovered from plasma were characterized by established techniques for EVs analysis including nanoparticle tracking and transmission electron microscopy.<br>

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

2013 ◽  
Vol 8 (3) ◽  
pp. 352-362 ◽  
Author(s):  
Paula A. J. Rosa ◽  
Ana M. Azevedo ◽  
S. Sommerfeld ◽  
Martina Mutter ◽  
Werner Bäcker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Precipitation of VEGF from mesenchymal stem cell culture supernatant using the PCA process

2019 ◽  
Vol 151 ◽  
pp. 40-48 ◽  
Author(s):  
Hee Jeong Park ◽  
Tae Jun Yoon ◽  
Won-Su Son ◽  
Chan-Ju Lee ◽  
Si-na Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Mesenchymal Stem Cell Culture ◽  
Stem Cell Culture

scholarly journals Design and Manufacturing of Electromechanical Chip for Rapid Measurement of IgG1 Antibody in Cell-Culture Supernatant

2016 ◽  
Vol 08 (07) ◽  
Author(s):  
Azam Rahimpour ◽  
Fatemeh Yazdian ◽  
Zahra Akbari Jonoush ◽  
Masoumeh Rajabibazl ◽  
Meisam Omi
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Rapid Measurement ◽  
Igg1 Antibody ◽  
Design And Manufacturing
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