scholarly journals In planta transformation of Brassica rapa and B. napus via vernalization-infiltration methods

2013 ◽  
Author(s):  
Yuke He ◽  
Jinjuan Bai ◽  
Feijie Wu ◽  
Yanfei Mao ◽  
Yuke He
Keyword(s):  
Brassica Rapa ◽  
In Planta Transformation ◽  
In Planta

A rapid, novel and high-throughput identification of putative bell pepper transformants generated through in planta transformation approach

2011 ◽  
Vol 129 (4) ◽  
pp. 898-903 ◽  
Author(s):  
Arthikala Manoj Kumar ◽  
Kalpana N. Reddy ◽  
M. Manjulatha ◽  
Elizabeth S. Arellano ◽  
Rohini Sreevathsa ◽  
...  
Keyword(s):  
High Throughput ◽  
Bell Pepper ◽  
In Planta Transformation ◽  
In Planta ◽  
Transformation Approach

scholarly journals Genetic and physiological regulation of folate in pak choi (Brassica rapa subsp. Chinensis) germplasm

2020 ◽  
Vol 71 (16) ◽  
pp. 4914-4929
Author(s):  
M J I Shohag ◽  
Yanyan Wei ◽  
Jie Zhang ◽  
Ying Feng ◽  
Michael Rychlik ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Transcriptional Level ◽  
In Planta ◽  
Physiological Basis ◽  
Breeding Programs ◽  
Pak Choi ◽  
Physiological Regulation ◽  
The Difference ◽  
Folate Homeostasis ◽  
Living Organisms

Abstract Folates are one of the essential micronutrients for all living organisms. Due to inadequate dietary intake, folate deficiency remains prevalent in humans. Genetically diverse germplasms can potentially be used as parents in breeding programs and also for understanding the folate regulatory network. Therefore, we investigated the natural genetic diversity of folates and their physiological regulation in pak choi (Brassica rapa subsp. Chinensis) germplasm. The total folate concentration ranged from 52.7 μg 100 gFW–1 to 166.9 μg 100 gFW–1, with 3.2-fold variation. The main folate vitamer was represented by 5-CH3-H4folate, with 4.5-fold variation. The activities of GTP cyclohydrolase I and aminodeoxy chorismate synthase, the first step of folate synthesis, were high in high folate accessions and low in low folate accessions. Analysis of the transcription levels of 11 genes associated with folate metabolism demonstrated that the difference in folate concentrations may be primarily controlled at the post-transcriptional level. A general correlation between total folate and their precursors was observed. Folate diversity and chlorophyll content were tightly regulated through the methyl cycle. The diverse genetic variation in pak choi germplasm indicated the great genetic potential to integrate breeding programs for folate biofortification and unravel the physiological basis of folate homeostasis in planta.

Agrobacterium-mediated in planta transformation of maize germ cells

2010 ◽  
Vol 46 (4) ◽  
pp. 501-504 ◽  
Author(s):  
E. M. Mamontova ◽  
V. A. Velikov ◽  
I. V. Volokhina ◽  
M. I. Chumakov
Keyword(s):  
Germ Cells ◽  
In Planta Transformation ◽  
In Planta ◽  
Maize Germ

Development of simple and efficient in Planta transformation method for wheat (Triticum aestivum L.) using Agrobacterium tumefaciens

2006 ◽  
Vol 102 (3) ◽  
pp. 162-170 ◽  
Author(s):  
Putu Supartana ◽  
Tsutomu Shimizu ◽  
Masahiro Nogawa ◽  
Hidenari Shioiri ◽  
Tadashi Nakajima ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Agrobacterium Tumefaciens ◽  
Triticum Aestivum L ◽  
Transformation Method ◽  
In Planta Transformation ◽  
In Planta

scholarly journals Tissue culture-based Agrobacterium-mediated and in planta transformation methods

2017 ◽  
Vol 53 (No. 4) ◽  
pp. 133-143 ◽  
Author(s):  
M. Niazian ◽  
S.A. Sadat Noori ◽  
P. Galuszka ◽  
S.M.M. Mortazavian
Keyword(s):  
Tissue Culture ◽  
Transformation Method ◽  
Gene Transformation ◽  
In Planta Transformation ◽  
In Planta ◽  
Agrobacterium Mediated Transformation ◽  
Advantages And Disadvantages ◽  
Culture Independent ◽  
Transformation Methods ◽  
Mutant Lines

Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method. The main problem of Agrobacterium-method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. Development of genotype-independent gene transformation method is of great interest in many plants. Some tissue culture-independent Agrobacterium-mediated gene transformation methods are reported in individual plants and crops. Generally, these methods are called in planta gene transformation. In planta transformation methods are free from somaclonal variation and easier, quicker, and simpler than tissue culture-based transformation methods. Vacuum infiltration, injection of Agrobacterium culture to plant tissues, pollen-tube pathway, floral dip and floral spray are the main methods of in planta transformation. Each of these methods has its own advantages and disadvantages. Simplicity and reliability are the primary reasons for the popularity of the in planta methods. These methods are much quicker than regular tissue culture-based Agrobacterium-mediated gene transformation and success can be achieved by non-experts. In the present review, we highlight all methods of in planta transformation comparing them with regular tissue culture-based Agrobacterium-mediated transformation methods and then recently successful transformations using these methods are presented.

Sign in / Sign up

Export Citation Format

Share Document