Silk 3D matrices incorporating human neural progenitor cells for neural tissue engineering applications

2015 ◽  
Vol 47 (12) ◽  
pp. 819-825 ◽  
Author(s):  
Bano Subia ◽  
Raj R Rao ◽  
Subhas C Kundu
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neural Tissue ◽  
Neural Progenitor ◽  
Neural Tissue Engineering ◽  
Engineering Applications ◽  
Human Neural Progenitor Cells

scholarly journals Chromosome 7 and 19 Trisomy in Cultured Human Neural Progenitor Cells

PLoS ONE ◽  
2009 ◽  
Vol 4 (10) ◽  
pp. e7630 ◽  
Author(s):  
Dhruv Sareen ◽  
Erin McMillan ◽  
Allison D. Ebert ◽  
Brandon C. Shelley ◽  
Julie A. Johnson ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Chromosome 7 ◽  
Human Neural Progenitor Cells

scholarly journals Differential epigenetic effects of chlorpyrifos and arsenic in proliferating and differentiating human neural progenitor cells

2016 ◽  
Vol 65 ◽  
pp. 212-223 ◽  
Author(s):  
Hee Yeon Kim ◽  
Susanna H. Wegner ◽  
Kirk P. Van Ness ◽  
Julie Juyoung Park ◽  
Sara E. Pacheco ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Epigenetic Effects ◽  
Human Neural Progenitor Cells

scholarly journals Electrospun Polyvinylidene Fluoride-Based Fibrous Scaffolds with Piezoelectric Characteristics for Bone and Neural Tissue Engineering

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 952 ◽  
Author(s):  
Li ◽  
Liao ◽  
Tjong
Keyword(s):  
Tissue Engineering ◽  
Polyvinylidene Fluoride ◽  
Neural Tissue ◽  
Electrospinning Process ◽  
Neural Cells ◽  
Neural Tissue Engineering ◽  
Engineering Applications ◽  
Pore Sizes ◽  
Β Phase ◽  
Small Pore

Polyvinylidene fluoride (PVDF) and polyvinylidene fluoride-trifluoroethylene (P(VDF-TrFE) with excellent piezoelectricity and good biocompatibility are attractive materials for making functional scaffolds for bone and neural tissue engineering applications. Electrospun PVDF and P(VDF-TrFE) scaffolds can produce electrical charges during mechanical deformation, which can provide necessary stimulation for repairing bone defects and damaged nerve cells. As such, these fibrous mats promote the adhesion, proliferation and differentiation of bone and neural cells on their surfaces. Furthermore, aligned PVDF and P(VDF-TrFE) fibrous mats can enhance neurite growth along the fiber orientation direction. These beneficial effects derive from the formation of electroactive, polar β-phase having piezoelectric properties. Polar β-phase can be induced in the PVDF fibers as a result of the polymer jet stretching and electrical poling during electrospinning. Moreover, the incorporation of TrFE monomer into PVDF can stabilize the β-phase without mechanical stretching or electrical poling. The main drawbacks of electrospinning process for making piezoelectric PVDF-based scaffolds are their small pore sizes and the use of highly toxic organic solvents. The small pore sizes prevent the infiltration of bone and neuronal cells into the scaffolds, leading to the formation of a single cell layer on the scaffold surfaces. Accordingly, modified electrospinning methods such as melt-electrospinning and near-field electrospinning have been explored by the researchers to tackle this issue. This article reviews recent development strategies, achievements and major challenges of electrospun PVDF and P(VDF-TrFE) scaffolds for tissue engineering applications.

scholarly journals Xenotransplantation of Human Neural Progenitor Cells to the Subretinal Space of Nonimmunosuppressed Pigs

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Karin Warfvinge ◽  
Philip H. Schwartz ◽  
Jens Folke Kiilgaard ◽  
Morten la Cour ◽  
Michael J. Young ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Mononuclear Cell ◽  
Neural Progenitor Cells ◽  
Retinal Vasculitis ◽  
Donor Cell ◽  
Neural Progenitor ◽  
Morphological Integration ◽  
Subretinal Space ◽  
Donor Cells ◽  
Human Neural Progenitor Cells

To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10–28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10–13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model.

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