scholarly journals Adipose Tissue as a Potential Source of Hematopoietic Stem/Progenitor Cells

Obesity ◽  
2012 ◽  
Vol 20 (5) ◽  
pp. 923-931 ◽  
Author(s):  
Wojciech Błogowski ◽  
Mariusz Z. Ratajczak ◽  
Ewelina Żyżniewska-Banaszak ◽  
Barbara Dołęgowska ◽  
Teresa Starzyńska
Keyword(s):  
Adipose Tissue ◽  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Potential Source

Adipose tissue is an extramedullary reservoir for functional hematopoietic stem and progenitor cells

Blood ◽  
2010 ◽  
Vol 115 (5) ◽  
pp. 957-964 ◽  
Author(s):  
Jinah Han ◽  
Young Jun Koh ◽  
Hye Rin Moon ◽  
Hyun Gee Ryoo ◽  
Chung-Hyun Cho ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Progenitor Cells ◽  
High Efficiency ◽  
Stromal Vascular Fraction ◽  
Hematopoietic Stem ◽  
Blood Transplantation ◽  
Variable Extent ◽  
Stem And Progenitor Cells

Abstract The stromal vascular fraction (SVF) in adipose tissue contains a pool of various stem and progenitor cells, but the existence of hematopoietic stem and progenitor cells (HSPCs) in the SVF has not been seriously considered. We detected the presence of HSPCs in the SVF by phenotypically probing with Lin−Sca-1+c-kit+ (LSK) and functionally confirming the presence using colony-forming cell assay and assessing the long-term multilineage reconstitution ability after SVF transplantation. The LSK population in the SVF was 0.004% plus or minus 0.001%, and 5 × 105 freshly isolated SVF cells gave rise to 13 plus or minus 4 multilineage colonies. In addition, 0.15% plus or minus 0.03% of SVF cells was home to bone marrow (BM), especially near vascular and endosteal regions, 24 hours after blood transplantation. SVF transplantation was capable of generating a long-term (> 16 weeks), but variable extent (2.1%-32.1%) multilineage reconstitution in primary recipients, which was subsequently transferred to the secondary recipients by BM transplantation. All HSPCs within the SVF originated from the BM. Furthermore, the granulocyte–colony-stimulating factor (G-CSF) mobilization of HSPCs from BM markedly elevated the number of phenotypic and functional HSPCs in the SVF, which induced a high efficiency long-term reconstitution in multilineage hematopoiesis in vivo. Our results provide compelling evidence that adipose tissue is a novel extramedullary tissue possessing phenotypic and functional HSPCs.

Human periprostatic white adipose tissue is rich in stromal progenitor cells and a potential source of prostate tumor stroma

2012 ◽  
Vol 237 (10) ◽  
pp. 1155-1162 ◽  
Author(s):  
Ricardo Ribeiro ◽  
Cátia Monteiro ◽  
Ricardo Silvestre ◽  
Ângela Castela ◽  
Helena Coutinho ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Progenitor Cells ◽  
White Adipose Tissue ◽  
Tumor Stroma ◽  
Prostate Tumor ◽  
Potential Source ◽  
Stromal Progenitor Cells

scholarly journals Spatial and biochemical interactions between bone marrow adipose tissue and hematopoietic stem and progenitor cells in rhesus macaques

Bone ◽  
2020 ◽  
Vol 133 ◽  
pp. 115248 ◽  
Author(s):  
Jacob J. Robino ◽  
Nathalie Pamir ◽  
Sara Rosario ◽  
Lindsey B. Crawford ◽  
Benjamin J. Burwitz ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adipose Tissue ◽  
Progenitor Cells ◽  
Rhesus Macaques ◽  
Hematopoietic Stem ◽  
Bone Marrow Adipose Tissue ◽  
Marrow Adipose Tissue ◽  
Stem And Progenitor Cells

scholarly journals Metabolic dysfunction induced by high-fat diet modulates hematopoietic stem and myeloid progenitor cells in brown adipose tissue of mice

2021 ◽  
Author(s):  
Kyle T Mincham ◽  
Kunjal Panchal ◽  
Prue H Hart ◽  
Robyn M Lucas ◽  
Martin Feelisch ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adipose Tissue ◽  
Progenitor Cells ◽  
Immune Cells ◽  
High Fat Diet ◽  
Low Dose ◽  
Metabolic Dysfunction ◽  
High Fat ◽  
Hematopoietic Stem ◽  
Brown Adipose

AbstractBrown adipose tissue (BAT) may be an important metabolic regulator of whole-body glucose. While important roles have been ascribed to macrophages in regulating metabolic functions in BAT, little known is known of the roles of other immune cells subsets, particularly dendritic cells (DCs). Eating a high fat diet may compromise the development of hematopoietic stem and progenitor cells (HSPC) – which give rise to DCs – in bone marrow, with less known of its effects in BAT. We have previously demonstrated that ongoing exposure to low-dose ultraviolet radiation (UVR) significantly reduced the ‘whitening’ effect of eating a high-fat diet upon interscapular (i)BAT of mice. Here, we examined whether this observation may be linked to changes in the phenotype of HSPC and myeloid-derived immune cells in iBAT and bone marrow of mice using 12-colour flow cytometry. Many HSPC subsets declined in both iBAT and bone marrow with increasing metabolic dysfunction. Conversely, with rising adiposity and metabolic dysfunction, conventional (c)DCs increased in both of these tissues. When compared to low-fat diet, consumption of high-fat diet significantly reduced proportions of myeloid, common myeloid and megakaryocyte-erythrocyte progenitors in iBAT, and short-term hematopoietic stem cells in bone marrow. In mice fed a high-fat diet, exposure to low-dose UVR significantly reduced proportions of cDCs in iBAT, independently of nitric oxide release from irradiated skin (blocked using the scavenger, cPTIO), but did not significantly modify HSPC subsets in either tissue. Further studies are needed to determine whether changes in these cell populations contribute towards metabolic dysfunction.

Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells

2001 ◽  
Vol 38 (2) ◽  
pp. 139-147
Author(s):  
Jan W. Gratama ◽  
D. Robert Sutherland ◽  
Michael Keeney
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Flow Cytometric ◽  
Stem And Progenitor Cells
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