scholarly journals Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5

2013 ◽  
Vol 20 (7) ◽  
pp. 892-899 ◽  
Author(s):  
Susanne A Kassube ◽  
Martin Jinek ◽  
Jie Fang ◽  
Susan Tsutakawa ◽  
Eva Nogales
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Regulation ◽  
Dna Helicase

Structure of an RNA polymerase II–RNA inhibitor complex elucidates transcription regulation by noncoding RNAs

2005 ◽  
Vol 13 (1) ◽  
pp. 44-48 ◽  
Author(s):  
Hubert Kettenberger ◽  
Alexander Eisenführ ◽  
Florian Brueckner ◽  
Mirko Theis ◽  
Michael Famulok ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Regulation ◽  
Noncoding Rnas ◽  
Inhibitor Complex

scholarly journals Genome‐wide analysis of FOXO3 mediated transcription regulation through RNA polymerase II profiling

2013 ◽  
Vol 9 (1) ◽  
pp. 638 ◽  
Author(s):  
Astrid Eijkelenboom ◽  
Michal Mokry ◽  
Elzo de Wit ◽  
Lydia M Smits ◽  
Paulien E Polderman ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Regulation ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Nuclear DNA helicase II is recruited to IFN-α–activated transcription sites at PML nuclear bodies

2002 ◽  
Vol 158 (3) ◽  
pp. 463-473 ◽  
Author(s):  
Beata Fuchsová ◽  
Petr Novák ◽  
Jarmila Kafková ◽  
Pavel Hozák
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Nuclear Dna ◽  
Dna Helicase ◽  
Nuclear Bodies ◽  
Interferon Α ◽  
Small Subset ◽  
Ultrastructural Studies ◽  
Dna Helicase Ii ◽  
Pml Nuclear Bodies

It is known that nuclear DNA helicase II (NDH II) links CREB-binding protein directly to RNA polymerase II holoenzyme, and that this interaction is essential for gene activation by CREB. Here, we report for the first time that some NDH II/RNA helicase A is a component of promyelocytic leukemia nuclear bodies (PML NBs). An autoimmune serum specific for PML NBs was identified and used in immunoprecipitation experiments. NDH II was present in the immunoprecipitates as shown by mass spectrometry and by immunoblotting. Immunofluorescence and ultrastructural studies showed that NDH II colocalizes with a small subset of PML NBs in control cells, however, colocalizes with practically all bodies in interferon-α–stimulated cells. After interferon stimulation, more PML NBs were found to contain newly synthesized RNA, as indicated by bromouridine incorporation. PML NBs also contain RNA polymerase II. The association of NDH II with PML NBs was transcriptionally dependent, and NDH II was present in all bodies with nascent RNA. Blocking of mRNA synthesis caused NDH II relocalization from nucleoplasm to nucleoli. Based on the data, we suggest that NDH II recruitment to PML NBs is connected with transcriptional regulation of interferon-α–inducible genes attached to PML NBs.

scholarly journals Emerging Views on the CTD Code

2012 ◽  
Vol 2012 ◽  
pp. 1-19 ◽  
Author(s):  
David W. Zhang ◽  
Juan B. Rodríguez-Molina ◽  
Joshua R. Tietjen ◽  
Corey M. Nemec ◽  
Aseem Z. Ansari
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Regulation ◽  
Chromatin Remodeling ◽  
Posttranslational Modifications ◽  
Rna Processing ◽  
Transcription Initiation ◽  
Protein Complexes ◽  
Pol Ii

The C-terminal domain (CTD) of RNA polymerase II (Pol II) consists of conserved heptapeptide repeats that function as a binding platform for different protein complexes involved in transcription, RNA processing, export, and chromatin remodeling. The CTD repeats are subject to sequential waves of posttranslational modifications during specific stages of the transcription cycle. These patterned modifications have led to the postulation of the “CTD code” hypothesis, where stage-specific patterns define a spatiotemporal code that is recognized by the appropriate interacting partners. Here, we highlight the role of CTD modifications in directing transcription initiation, elongation, and termination. We examine the major readers, writers, and erasers of the CTD code and examine the relevance of describing patterns of posttranslational modifications as a “code.” Finally, we discuss major questions regarding the function of the newly discovered CTD modifications and the fundamental insights into transcription regulation that will necessarily emerge upon addressing those challenges.

scholarly journals Transcription Regulation by the Noncoding RNA SRG1 Requires Spt2-Dependent Chromatin Deposition in the Wake of RNA Polymerase II

2011 ◽  
Vol 31 (6) ◽  
pp. 1288-1300 ◽  
Author(s):  
P. Thebault ◽  
G. Boutin ◽  
W. Bhat ◽  
A. Rufiange ◽  
J. Martens ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Regulation ◽  
Noncoding Rna

scholarly journals A Role for the TFIIH XPB DNA Helicase in Promoter Escape by RNA Polymerase II

1999 ◽  
Vol 274 (32) ◽  
pp. 22127-22130 ◽  
Author(s):  
Rodney J. Moreland ◽  
Franck Tirode ◽  
Qin Yan ◽  
Joan Weliky Conaway ◽  
Jean-Marc Egly ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Dna Helicase ◽  
Promoter Escape
Sign in / Sign up

Export Citation Format

Share Document