Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS

2009 ◽  
Vol 16 (4) ◽  
pp. 390-396 ◽  
Author(s):  
Salvatore Adinolfi ◽  
Clara Iannuzzi ◽  
Filippo Prischi ◽  
Chiara Pastore ◽  
Stefania Iametti ◽  
...  
Keyword(s):  
Cluster Formation ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

Regulation of Iron-Sulfur Cluster Formation in Plastids

2012 ◽  
pp. 135-144
Author(s):  
Karl Ravet ◽  
Douglas Van Hoewyk ◽  
Marinus Pilon
Keyword(s):  
Cluster Formation ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

Methylene insertion into an Fe2S2 cluster: formation of a thiolate-bridged diiron complex containing an Fe–CH2–S moiety

2018 ◽  
Vol 54 (93) ◽  
pp. 13119-13122 ◽  
Author(s):  
Linan Su ◽  
Dawei Yang ◽  
Yixin Zhang ◽  
Baomin Wang ◽  
Jingping Qu
Keyword(s):  
Cluster Formation ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

Cleavage of both C−Cl bonds in CH2Cl2 by a reduced iron–sulfur cluster induced unexpected methylene insertion into the Fe–S bond.

scholarly journals The Hsp70 Chaperone Ssq1p Is Dispensable for Iron-Sulfur Cluster Formation on the Scaffold Protein Isu1p

2006 ◽  
Vol 281 (12) ◽  
pp. 7801-7808 ◽  
Author(s):  
Rafal Dutkiewicz ◽  
Jaroslaw Marszalek ◽  
Brenda Schilke ◽  
Elizabeth A. Craig ◽  
Roland Lill ◽  
...  
Keyword(s):  
Cluster Formation ◽  
Scaffold Protein ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Hsp70 Chaperone

scholarly journals In vivo iron-sulfur cluster formation

2008 ◽  
Vol 105 (25) ◽  
pp. 8591-8596 ◽  
Author(s):  
E. C. Raulfs ◽  
I. P. O'Carroll ◽  
P. C. Dos Santos ◽  
M.-C. Unciuleac ◽  
D. R. Dean
Keyword(s):  
Cluster Formation ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

scholarly journals Molecular mechanism of the dual regulation of bacterial iron sulfur cluster biogenesis by CyaY and IscX

2017 ◽  
Author(s):  
Salvatore Adinolfi ◽  
Rita Puglisi ◽  
Jason C. Crack ◽  
Clara Iannuzzi ◽  
Fabrizio Dal Piaz ◽  
...  
Keyword(s):  
Metabolic Pathway ◽  
Cluster Formation ◽  
Hybrid Approach ◽  
Cluster Assembly ◽  
Enzymatic Process ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Binding Surface ◽  
Iron Concentrations

AbstractIscX (or YfhJ) is a protein of unknown function which takes part in the iron-sulfur cluster assembly machinery, a highly specialised and essential metabolic pathway. IscX binds to iron with low affinity and interacts with IscS, the desulfurase central to cluster assembly. Previous studies have suggested a competition between IscX and CyaY, the bacterial ortholog of frataxin, for the same binding surface of IscS. This competition could suggest a link between the two proteins with a functional significance. Using a hybrid approach, we show here that IscX is a modulator of the inhibitory properties of CyaY: by competing for the same site on IscS, the presence of IscX rescues the rates of enzymatic cluster formation which are inhibited by CyaY. The effect is stronger at low iron concentrations, whereas it becomes negligible at high iron concentrations. These results strongly suggest that iron-sulfur cluster assembly is an exquisite example of an enzymatic process which requires a double regulation under the control of iron as the effector.

scholarly journals Copper Stress Affects Iron Homeostasis by Destabilizing Iron-Sulfur Cluster Formation in Bacillus subtilis

2010 ◽  
Vol 192 (10) ◽  
pp. 2512-2524 ◽  
Author(s):  
Shashi Chillappagari ◽  
Andreas Seubert ◽  
Hein Trip ◽  
Oscar P. Kuipers ◽  
Mohamed A. Marahiel ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Iron Homeostasis ◽  
Cluster Formation ◽  
Cellular Growth ◽  
Copper Stress ◽  
Intracellular Iron ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Copper Excess

ABSTRACT Copper and iron are essential elements for cellular growth. Although bacteria have to overcome limitations of these metals by affine and selective uptake, excessive amounts of both metals are toxic for the cells. Here we investigated the influences of copper stress on iron homeostasis in Bacillus subtilis, and we present evidence that copper excess leads to imbalances of intracellular iron metabolism by disturbing assembly of iron-sulfur cofactors. Connections between copper and iron homeostasis were initially observed in microarray studies showing upregulation of Fur-dependent genes under conditions of copper excess. This effect was found to be relieved in a csoR mutant showing constitutive copper efflux. In contrast, stronger Fur-dependent gene induction was found in a copper efflux-deficient copA mutant. A significant induction of the PerR regulon was not observed under copper stress, indicating that oxidative stress did not play a major role under these conditions. Intracellular iron and copper quantification revealed that the total iron content was stable during different states of copper excess or efflux and hence that global iron limitation did not account for copper-dependent Fur derepression. Strikingly, the microarray data for copper stress revealed a broad effect on the expression of genes coding for iron-sulfur cluster biogenesis (suf genes) and associated pathways such as cysteine biosynthesis and genes coding for iron-sulfur cluster proteins. Since these effects suggested an interaction of copper and iron-sulfur cluster maturation, a mutant with a conditional mutation of sufU, encoding the essential iron-sulfur scaffold protein in B. subtilis, was assayed for copper sensitivity, and its growth was found to be highly susceptible to copper stress. Further, different intracellular levels of SufU were found to influence the strength of Fur-dependent gene expression. By investigating the influence of copper on cluster-loaded SufU in vitro, Cu(I) was found to destabilize the scaffolded cluster at submicromolar concentrations. Thus, by interfering with iron-sulfur cluster formation, copper stress leads to enhanced expression of cluster scaffold and target proteins as well as iron and sulfur acquisition pathways, suggesting a possible feedback strategy to reestablish cluster biogenesis.

scholarly journals YfhJ, a Molecular Adaptor in Iron-Sulfur Cluster Formation or a Frataxin-like Protein?

Structure ◽  
2006 ◽  
Vol 14 (5) ◽  
pp. 857-867 ◽  
Author(s):  
Chiara Pastore ◽  
Salvatore Adinolfi ◽  
Martjin A. Huynen ◽  
Vladimir Rybin ◽  
Stephen Martin ◽  
...  
Keyword(s):  
Cluster Formation ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

Gene Cloning, Purification, and Characterization of Two Cyanobacterial NifS Homologs Driving Iron-Sulfur Cluster Formation

2000 ◽  
Vol 64 (11) ◽  
pp. 2412-2419 ◽  
Author(s):  
Shin-ichiro KATO ◽  
Hisaaki MIHARA ◽  
Tatsuo KURIHARA ◽  
Tohru YOSHIMURA ◽  
Nobuyoshi ESAKI
Keyword(s):  
Gene Cloning ◽  
Cluster Formation ◽  
Purification And Characterization ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur

The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron?sulfur cluster formation in ferredoxin

Planta ◽  
2004 ◽  
Vol 220 (4) ◽  
pp. 602-608 ◽  
Author(s):  
Hong Ye ◽  
Gulnara F. Garifullina ◽  
Salah E. Abdel-Ghany ◽  
Lihong Zhang ◽  
Elizabeth A. H. Pilon-Smits ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cluster Formation ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur
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