Gene Cloning, Purification, and Characterization of Two Cyanobacterial NifS Homologs Driving Iron-Sulfur Cluster Formation

2000 ◽  
Vol 64 (11) ◽  
pp. 2412-2419 ◽  
Author(s):  
Shin-ichiro KATO ◽  
Hisaaki MIHARA ◽  
Tatsuo KURIHARA ◽  
Tohru YOSHIMURA ◽  
Nobuyoshi ESAKI
2007 ◽  
Vol 20-21 ◽  
pp. 509-512 ◽  
Author(s):  
Jian She Liu ◽  
Yan Fei Zhang ◽  
Mei Mei Geng ◽  
Jia Zeng ◽  
Guan Zhou Qiu

The highly conserved operon iron–sulfur cluster (iscSUA) is essential for the general biogenesis and transfer of iron–sulfur proteins in bacteria. In this study, expression, purification and characterization of the proteins of the isc operon (iscSUA) of Acidithiobacillus ferrooxidans ATCC 23270 was studied. Assembly and transfer of [Fe4S4] in vitro during the isc proteins and other iron sulfur proteins was studied in order to detect the pathway and mechanism of [Fe4S4] assembly and transfer in vivo. The [Fe4S4] cluster was successfully assembled in iron-sulfur proteins in vitro in the presence of Fe2+ and sulfide, and it was successfully transferred from IscA or IscU to iron- sulfur proteins. Our results support and extend certain models of iron-sulfur clusters assembly and transfer.


1997 ◽  
Vol 36 (2) ◽  
pp. 214-219 ◽  
Author(s):  
Yuheng Deng ◽  
Qiutian Liu ◽  
Yu Yang ◽  
Youtong Wang ◽  
Yuanba Cai ◽  
...  

2012 ◽  
pp. 135-144
Author(s):  
Karl Ravet ◽  
Douglas Van Hoewyk ◽  
Marinus Pilon

1981 ◽  
Vol 20 (11) ◽  
pp. 3586-3589 ◽  
Author(s):  
Pierre. Braunstein ◽  
Antonio. Tiripicchio ◽  
Marisa. Tiripicchio Camellini ◽  
Enrico. Sappa

Sign in / Sign up

Export Citation Format

Share Document