Long-term survival during stationary phase: evolution and the GASP phenotype

2006 ◽  
Vol 4 (2) ◽  
pp. 113-120 ◽  
Author(s):  
Steven E. Finkel
Keyword(s):  
Stationary Phase ◽  
Phase Evolution ◽  
Term Survival ◽  
Long Term Survival

scholarly journals Anaerobic Survival of Pseudomonas aeruginosa by Pyruvate Fermentation Requires an Usp-Type Stress Protein

2006 ◽  
Vol 188 (2) ◽  
pp. 659-668 ◽  
Author(s):  
Kerstin Schreiber ◽  
Nelli Boes ◽  
Martin Eschbach ◽  
Lothar Jaensch ◽  
Juergen Wehland ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stationary Phase ◽  
Gene Fusion ◽  
Laser Scanning Microscopy ◽  
Green Fluorescent Protein Gene ◽  
Confocal Laser ◽  
Term Survival ◽  
Long Term Survival ◽  
Pyruvate Fermentation

ABSTRACT Recently, we identified a pyruvate fermentation pathway in Pseudomonas aeruginosa sustaining anaerobic survival in the absence of alternative anaerobic respiratory and fermentative energy generation systems (M. Eschbach, K. Schreiber, K. Trunk, J. Buer, D. Jahn, and M. Schobert, J. Bacteriol. 186:4596-4604, 2004). Anaerobic long-term survival of P. aeruginosa might be essential for survival in deeper layers of a biofilm and the persistent infection of anaerobic mucus plaques in the cystic fibrosis lung. Proteome analysis of P. aeruginosa cells during a 7-day period of pyruvate fermentation revealed the induced synthesis of three enzymes involved in arginine fermentation, ArcA, ArcB, and ArcC, and the outer membrane protein OprL. Moreover, formation of two proteins of unknown function, PA3309 and PA4352, increased by factors of 72- and 22-fold, respectively. Both belong to the group of universal stress proteins (Usp). Long-term survival of a PA3309 knockout mutant by pyruvate fermentation was found drastically reduced. The oxygen-sensing regulator Anr controls expression of the P PA3309-lacZ reporter gene fusion after a shift to anaerobic conditions and further pyruvate fermentation. PA3309 expression was also found induced during the anaerobic and aerobic stationary phases. This aerobic stationary-phase induction is independent of the regulatory proteins Anr, RpoS, RelA, GacA, RhlR, and LasR, indicating a currently unknown mechanism of stationary-phase-dependent gene activation. PA3309 promoter activity was detected in the deeper layers of a P. aeruginosa biofilm using a P PA3309-gfp (green fluorescent protein gene) fusion and confocal laser-scanning microscopy. This is the first description of an Anr-dependent, anaerobically induced, and functional Usp-like protein in bacteria.

scholarly journals A metabolic strategy to enhance long-term survival by Phx1 through stationary phase-specific pyruvate decarboxylases in fission yeast

Aging ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 587-601 ◽  
Author(s):  
Ji-Yoon Kim ◽  
Eun-Jung Kim ◽  
Luis Lopez-Maury ◽  
Jürg Bähler ◽  
Jung-Hye Roe
Keyword(s):  
Stationary Phase ◽  
Fission Yeast ◽  
Term Survival ◽  
Long Term Survival

scholarly journals Culture Volume and Vessel Affect Long-Term Survival, Mutation Frequency, and Oxidative Stress of Escherichia coli

2013 ◽  
Vol 80 (5) ◽  
pp. 1732-1738 ◽  
Author(s):  
Karin E. Kram ◽  
Steven E. Finkel
Keyword(s):  
Oxidative Stress ◽  
Escherichia Coli ◽  
Stationary Phase ◽  
Mutation Frequency ◽  
Physiological Responses ◽  
Culture Vessel ◽  
Term Survival ◽  
Long Term Survival ◽  
Content Type

ABSTRACTBacteria such asEscherichia coliare frequently studied during exponential- and stationary-phase growth. However, many strains can survive in long-term stationary phase (LTSP), without the addition of nutrients, from days to several years. During LTSP, cells experience a variety of stressors, including reactive oxidative species, nutrient depletion, and metabolic toxin buildup, that lead to physiological responses and changes in genetic stability. In this study, we monitored survival during LTSP, as well as reporters of genetic and physiological change, to determine how the physical environment affectsE. coliduring long-term batch culture. We demonstrate differences in yield during LTSP in cells incubated in LB medium in test tubes versus Erlenmeyer flasks, as well as growth in different volumes of medium. We determined that these differences are only partially due to differences in oxygen levels by incubating the cells in different volumes of media under anaerobic conditions. Since we hypothesized that differences in long-term survival are the result of changes in physiological outputs during the late log and early stationary phases, we monitored alkalization, mutation frequency, oxidative stress response, and glycation. Although initial cell yields are essentially equivalent under each condition tested, physiological responses vary greatly in response to culture environment. Incubation in lower-volume cultures leads to higheroxyRexpression but lower mutation frequency and glycation levels, whereas incubation in high-volume cultures has the opposite effect. We show here that even under commonly used experimental conditions that are frequently treated as equivalent, the stresses experienced by cells can differ greatly, suggesting that culture vessel and incubation conditions should be carefully considered in the planning or analysis of experiments.

Recovery from long-term stationary phase and stress survival in Escherichia coli require the l-isoaspartyl protein carboxyl methyltransferase at alkaline pH

Microbiology ◽  
2005 ◽  
Vol 151 (7) ◽  
pp. 2151-2158 ◽  
Author(s):  
Wade M. Hicks ◽  
Matthew V. Kotlajich ◽  
Jonathan E. Visick
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Alkaline Ph ◽  
Term Survival ◽  
Long Term Survival ◽  
E Coli ◽  
Carboxyl Methyltransferase ◽  
Protein Carboxyl Methyltransferase ◽  
Stress Survival

The l-isoaspartyl protein carboxyl methyltransferase (pcm) can stimulate repair of isoaspartyl residues arising spontaneously in proteins to normal l-aspartyl residues. PCM is needed in Escherichia coli for maximal long-term survival when exposed to oxidative stress, osmotic stress, repeated heat stress or methanol. The effect of pH on a pcm mutant during long-term stationary phase was examined. PCM was not required for long-term survival of E. coli subjected to pH stress alone; however, PCM-deficient cells showed impaired resistance to paraquat and methanol only at elevated pH. The mutant also showed stress-survival phenotypes in minimal medium buffered to pH 9·0. Accumulation of isoaspartyl residues was accelerated at pH 8·0 or 9·0 in vivo, though PCM-deficient cells did not show higher levels of damage. However, the pcm mutant displayed an extended lag phase in recovering from stationary phase at pH 9·0. Protein repair by PCM thus plays a key role in long-term stress survival only at alkaline pH in E. coli, and it may function primarily to repair damage in cells that are recovering from nutrient limitation and in those cells that are able to divide during long-term stationary phase.

Proteomics analysis of a long-term survival strain of Escherichia coli K-12 exhibiting a growth advantage in stationary-phase (GASP) phenotype

PROTEOMICS ◽  
2016 ◽  
Vol 16 (6) ◽  
pp. 963-972 ◽  
Author(s):  
Assunta Gagliardi ◽  
Egidio Lamboglia ◽  
Laura Bianchi ◽  
Claudia Landi ◽  
Alessandro Armini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Proteomics Analysis ◽  
Term Survival ◽  
Long Term Survival ◽  
K 12

scholarly journals Growth advantage in stationary-phase (GASP) phenotype in long-term survival strains of Geobacter sulfurreducens

2011 ◽  
Vol 79 (1) ◽  
pp. 218-228 ◽  
Author(s):  
Ruth A. Helmus ◽  
Laura J. Liermann ◽  
Susan L. Brantley ◽  
Ming Tien
Keyword(s):  
Stationary Phase ◽  
Geobacter Sulfurreducens ◽  
Term Survival ◽  
Long Term Survival

Human thymic epithelial cells maintain long-term survival of clonogenic myeloid and erythroid progenitor cells in vitro

2000 ◽  
Vol 111 (1) ◽  
pp. 363-370 ◽  
Author(s):  
Katsuto Takenaka ◽  
Mine Harada ◽  
Tomoaki Fujisaki ◽  
Koji Nagafuji ◽  
Shinichi Mizuno ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Progenitor Cells ◽  
Thymic Epithelial Cells ◽  
Erythroid Progenitor ◽  
Term Survival ◽  
Long Term Survival ◽  
Erythroid Progenitor Cells

Clinical outcome and long-term survival following radical esophagectomy in the elderly

2001 ◽  
Vol 120 (5) ◽  
pp. A747-A748
Author(s):  
S DRESNER ◽  
A IMMMANUEL ◽  
P LAMB ◽  
S GRIFFIN
Keyword(s):  
Clinical Outcome ◽  
The Elderly ◽  
Radical Esophagectomy ◽  
Term Survival ◽  
Long Term Survival
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