Proteomics analysis of a long-term survival strain of Escherichia coli K-12 exhibiting a growth advantage in stationary-phase (GASP) phenotype

PROTEOMICS ◽  
2016 ◽  
Vol 16 (6) ◽  
pp. 963-972 ◽  
Author(s):  
Assunta Gagliardi ◽  
Egidio Lamboglia ◽  
Laura Bianchi ◽  
Claudia Landi ◽  
Alessandro Armini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Proteomics Analysis ◽  
Term Survival ◽  
Long Term Survival ◽  
K 12

scholarly journals Culture Volume and Vessel Affect Long-Term Survival, Mutation Frequency, and Oxidative Stress of Escherichia coli

2013 ◽  
Vol 80 (5) ◽  
pp. 1732-1738 ◽  
Author(s):  
Karin E. Kram ◽  
Steven E. Finkel
Keyword(s):  
Oxidative Stress ◽  
Escherichia Coli ◽  
Stationary Phase ◽  
Mutation Frequency ◽  
Physiological Responses ◽  
Culture Vessel ◽  
Term Survival ◽  
Long Term Survival ◽  
Content Type

ABSTRACTBacteria such asEscherichia coliare frequently studied during exponential- and stationary-phase growth. However, many strains can survive in long-term stationary phase (LTSP), without the addition of nutrients, from days to several years. During LTSP, cells experience a variety of stressors, including reactive oxidative species, nutrient depletion, and metabolic toxin buildup, that lead to physiological responses and changes in genetic stability. In this study, we monitored survival during LTSP, as well as reporters of genetic and physiological change, to determine how the physical environment affectsE. coliduring long-term batch culture. We demonstrate differences in yield during LTSP in cells incubated in LB medium in test tubes versus Erlenmeyer flasks, as well as growth in different volumes of medium. We determined that these differences are only partially due to differences in oxygen levels by incubating the cells in different volumes of media under anaerobic conditions. Since we hypothesized that differences in long-term survival are the result of changes in physiological outputs during the late log and early stationary phases, we monitored alkalization, mutation frequency, oxidative stress response, and glycation. Although initial cell yields are essentially equivalent under each condition tested, physiological responses vary greatly in response to culture environment. Incubation in lower-volume cultures leads to higheroxyRexpression but lower mutation frequency and glycation levels, whereas incubation in high-volume cultures has the opposite effect. We show here that even under commonly used experimental conditions that are frequently treated as equivalent, the stresses experienced by cells can differ greatly, suggesting that culture vessel and incubation conditions should be carefully considered in the planning or analysis of experiments.

Recovery from long-term stationary phase and stress survival in Escherichia coli require the l-isoaspartyl protein carboxyl methyltransferase at alkaline pH

Microbiology ◽  
2005 ◽  
Vol 151 (7) ◽  
pp. 2151-2158 ◽  
Author(s):  
Wade M. Hicks ◽  
Matthew V. Kotlajich ◽  
Jonathan E. Visick
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Alkaline Ph ◽  
Term Survival ◽  
Long Term Survival ◽  
E Coli ◽  
Carboxyl Methyltransferase ◽  
Protein Carboxyl Methyltransferase ◽  
Stress Survival

The l-isoaspartyl protein carboxyl methyltransferase (pcm) can stimulate repair of isoaspartyl residues arising spontaneously in proteins to normal l-aspartyl residues. PCM is needed in Escherichia coli for maximal long-term survival when exposed to oxidative stress, osmotic stress, repeated heat stress or methanol. The effect of pH on a pcm mutant during long-term stationary phase was examined. PCM was not required for long-term survival of E. coli subjected to pH stress alone; however, PCM-deficient cells showed impaired resistance to paraquat and methanol only at elevated pH. The mutant also showed stress-survival phenotypes in minimal medium buffered to pH 9·0. Accumulation of isoaspartyl residues was accelerated at pH 8·0 or 9·0 in vivo, though PCM-deficient cells did not show higher levels of damage. However, the pcm mutant displayed an extended lag phase in recovering from stationary phase at pH 9·0. Protein repair by PCM thus plays a key role in long-term stress survival only at alkaline pH in E. coli, and it may function primarily to repair damage in cells that are recovering from nutrient limitation and in those cells that are able to divide during long-term stationary phase.

scholarly journals Anaerobic Survival of Pseudomonas aeruginosa by Pyruvate Fermentation Requires an Usp-Type Stress Protein

2006 ◽  
Vol 188 (2) ◽  
pp. 659-668 ◽  
Author(s):  
Kerstin Schreiber ◽  
Nelli Boes ◽  
Martin Eschbach ◽  
Lothar Jaensch ◽  
Juergen Wehland ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stationary Phase ◽  
Gene Fusion ◽  
Laser Scanning Microscopy ◽  
Green Fluorescent Protein Gene ◽  
Confocal Laser ◽  
Term Survival ◽  
Long Term Survival ◽  
Pyruvate Fermentation

ABSTRACT Recently, we identified a pyruvate fermentation pathway in Pseudomonas aeruginosa sustaining anaerobic survival in the absence of alternative anaerobic respiratory and fermentative energy generation systems (M. Eschbach, K. Schreiber, K. Trunk, J. Buer, D. Jahn, and M. Schobert, J. Bacteriol. 186:4596-4604, 2004). Anaerobic long-term survival of P. aeruginosa might be essential for survival in deeper layers of a biofilm and the persistent infection of anaerobic mucus plaques in the cystic fibrosis lung. Proteome analysis of P. aeruginosa cells during a 7-day period of pyruvate fermentation revealed the induced synthesis of three enzymes involved in arginine fermentation, ArcA, ArcB, and ArcC, and the outer membrane protein OprL. Moreover, formation of two proteins of unknown function, PA3309 and PA4352, increased by factors of 72- and 22-fold, respectively. Both belong to the group of universal stress proteins (Usp). Long-term survival of a PA3309 knockout mutant by pyruvate fermentation was found drastically reduced. The oxygen-sensing regulator Anr controls expression of the P PA3309-lacZ reporter gene fusion after a shift to anaerobic conditions and further pyruvate fermentation. PA3309 expression was also found induced during the anaerobic and aerobic stationary phases. This aerobic stationary-phase induction is independent of the regulatory proteins Anr, RpoS, RelA, GacA, RhlR, and LasR, indicating a currently unknown mechanism of stationary-phase-dependent gene activation. PA3309 promoter activity was detected in the deeper layers of a P. aeruginosa biofilm using a P PA3309-gfp (green fluorescent protein gene) fusion and confocal laser-scanning microscopy. This is the first description of an Anr-dependent, anaerobically induced, and functional Usp-like protein in bacteria.

scholarly journals Long-Term Survival of Shiga Toxin-ProducingEscherichia coli O26, O111, and O157 in Bovine Feces

1999 ◽  
Vol 65 (11) ◽  
pp. 5177-5181 ◽  
Author(s):  
Hiroshi Fukushima ◽  
Ken Hoshina ◽  
Manabu Gomyoda
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Term Survival ◽  
Long Term Survival ◽  
Low Concentration ◽  
Long Time ◽  
High Concentrations ◽  
Bovine Feces

ABSTRACT Cattle are an important reservoir of Shiga toxin-producingEscherichia coli (STEC) O26, O111, and O157. The fate of these pathogens in bovine feces at 5, 15, and 25°C was examined. The feces of a cow naturally infected with STEC O26:H11 and two STEC-free cows were studied. STEC O26, O111, and O157 were inoculated into bovine feces at 101, 103, and 105 CFU/g. All three pathogens survived at 5 and 25°C for 1 to 4 weeks and at 15°C for 1 to 8 weeks when inoculated at the low concentration. On samples inoculated with the middle and high concentrations, O26, O111, and O157 survived at 25°C for 3 to 12 weeks, at 15°C for 1 to 18 weeks, and at 5°C for 2 to 14 weeks, respectively. Therefore, these pathogens can survive in feces for a long time, especially at 15°C. The surprising long-term survival of STEC O26, O111, and O157 in bovine feces shows that such feces are a potential vehicle for transmitting not only O157 but also O26 and O111 to cattle, food, and the environment. Appropriate handling of bovine feces is emphasized.

scholarly journals A metabolic strategy to enhance long-term survival by Phx1 through stationary phase-specific pyruvate decarboxylases in fission yeast

Aging ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 587-601 ◽  
Author(s):  
Ji-Yoon Kim ◽  
Eun-Jung Kim ◽  
Luis Lopez-Maury ◽  
Jürg Bähler ◽  
Jung-Hye Roe
Keyword(s):  
Stationary Phase ◽  
Fission Yeast ◽  
Term Survival ◽  
Long Term Survival

scholarly journals Long-term survival of Escherichia coli O157 on pasture following an outbreak associated with sheep at a scout camp

2002 ◽  
Vol 34 (2) ◽  
pp. 100-104 ◽  
Author(s):  
I.D. Ogden ◽  
N.F. Hepburn ◽  
M. MacRae ◽  
N.J.C. Strachan ◽  
D.R. Fenlon ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Term Survival ◽  
Long Term Survival

scholarly journals Antiglycation Effects of Carnosine and Other Compounds on the Long-Term Survival of Escherichia coli

2010 ◽  
Vol 76 (24) ◽  
pp. 7925-7930 ◽  
Author(s):  
Evan D. Pepper ◽  
Michael J. Farrell ◽  
Gary Nord ◽  
Steven E. Finkel
Keyword(s):  
Escherichia Coli ◽  
Culture Medium ◽  
Term Survival ◽  
Long Term Survival ◽  
E Coli ◽  
Human Disorders ◽  
Domains Of Life ◽  
Carboxymethyl Lysine ◽  
Dose Dependent

ABSTRACT Glycation, or nonenzymatic glycosylation, is a chemical reaction between reactive carbonyl-containing compounds and biomolecules containing free amino groups. Carbonyl-containing compounds include reducing sugars such as glucose or fructose, carbohydrate-derived compounds such as methylglyoxal and glyoxal, and nonsugars such as polyunsaturated fatty acids. The latter group includes molecules such as proteins, DNA, and amino lipids. Glycation-induced damage to these biomolecules has been shown to be a contributing factor in human disorders such as Alzheimer's disease, atherosclerosis, and cataracts and in diabetic complications. Glycation also affects Escherichia coli under standard laboratory conditions, leading to a decline in bacterial population density and long-term survival. Here we have shown that as E. coli aged in batch culture, the amount of carboxymethyl lysine, an advanced glycation end product, accumulated over time and that this accumulation was affected by the addition of glucose to the culture medium. The addition of excess glucose or methylglyoxal to the culture medium resulted in a dose-dependent loss of cell viability. We have also demonstrated that glyoxylase enzyme GloA plays a role in cell survival during glycation stress. In addition, we have provided evidence that carnosine, folic acid, and aminoguanidine inhibit glycation in prokaryotes. These agents may also prove to be beneficial to eukaryotes since the chemical processes of glycation are similar in these two domains of life.

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