In vitro sperm production from mouse spermatogonial stem cell lines using an organ culture method

2013 ◽  
Vol 8 (11) ◽  
pp. 2098-2104 ◽  
Author(s):  
Takuya Sato ◽  
Kumiko Katagiri ◽  
Yoshinobu Kubota ◽  
Takehiko Ogawa
Keyword(s):  
Stem Cell ◽  
Organ Culture ◽  
Cell Lines ◽  
Culture Method ◽  
Spermatogonial Stem Cell ◽  
Sperm Production ◽  
Stem Cell Lines

scholarly journals In vitro production of fertile sperm from murine spermatogonial stem cell lines

2011 ◽  
Vol 2 (1) ◽  
Author(s):  
Takuya Sato ◽  
Kumiko Katagiri ◽  
Tetsuhiro Yokonishi ◽  
Yoshinobu Kubota ◽  
Kimiko Inoue ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Spermatogonial Stem Cell ◽  
In Vitro Production ◽  
Stem Cell Lines ◽  
Vitro Production

scholarly journals OP0073 ESTABLISHMENT OF HUMAN INDUCED PLURIPOTENT STEM CELL-LINES (IPSC) FOR IN VITRO MODELLING HAND OSTHEOARTHRITIS

2019 ◽  
Author(s):  
Rocío Castro-Viñuelas ◽  
Clara Sanjurjo-Rodríguez ◽  
María Piñeiro-Ramil ◽  
Tamara Hermida Gómez ◽  
Isaac Fuentes-Boquete ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Stem Cell Lines ◽  
Induced Pluripotent ◽  
In Vitro Modelling

Ex Uno Plures: Molecular Designs for Embryonic Pluripotency

2015 ◽  
Vol 95 (1) ◽  
pp. 245-295 ◽  
Author(s):  
Kyle M. Loh ◽  
Bing Lim ◽  
Lay Teng Ang
Keyword(s):  
Stem Cell ◽  
Transcription Factors ◽  
Cell Lines ◽  
Multilineage Differentiation ◽  
Pluripotent Cells ◽  
Differentiation Potential ◽  
Developmental Potential ◽  
Pluripotency Factors ◽  
Stem Cell Lines

Pluripotent cells in embryos are situated near the apex of the hierarchy of developmental potential. They are capable of generating all cell types of the mammalian body proper. Therefore, they are the exemplar of stem cells. In vivo, pluripotent cells exist transiently and become expended within a few days of their establishment. Yet, when explanted into artificial culture conditions, they can be indefinitely propagated in vitro as pluripotent stem cell lines. A host of transcription factors and regulatory genes are now known to underpin the pluripotent state. Nonetheless, how pluripotent cells are equipped with their vast multilineage differentiation potential remains elusive. Consensus holds that pluripotency transcription factors prevent differentiation by inhibiting the expression of differentiation genes. However, this does not explain the developmental potential of pluripotent cells. We have presented another emergent perspective, namely, that pluripotency factors function as lineage specifiers that enable pluripotent cells to differentiate into specific lineages, therefore endowing pluripotent cells with their multilineage potential. Here we provide a comprehensive overview of the developmental biology, transcription factors, and extrinsic signaling associated with pluripotent cells, and their accompanying subtypes, in vitro heterogeneity and chromatin states. Although much has been learned since the appreciation of mammalian pluripotency in the 1950s and the derivation of embryonic stem cell lines in 1981, we will specifically emphasize what currently remains unclear. However, the view that pluripotency factors capacitate differentiation, recently corroborated by experimental evidence, might perhaps address the long-standing question of how pluripotent cells are endowed with their multilineage differentiation potential.

scholarly journals Towards consistent generation of pancreatic lineage progenitors from human pluripotent stem cells

2015 ◽  
Vol 370 (1680) ◽  
pp. 20140365 ◽  
Author(s):  
Maria Rostovskaya ◽  
Nicholas Bredenkamp ◽  
Austin Smith
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cells ◽  
Type I ◽  
Pancreatic Progenitors ◽  
Stem Cell Lines

Human pluripotent stem cells can in principle be used as a source of any differentiated cell type for disease modelling, drug screening, toxicology testing or cell replacement therapy. Type I diabetes is considered a major target for stem cell applications due to the shortage of primary human beta cells. Several protocols have been reported for generating pancreatic progenitors by in vitro differentiation of human pluripotent stem cells. Here we first assessed one of these protocols on a panel of pluripotent stem cell lines for capacity to engender glucose sensitive insulin-producing cells after engraftment in immunocompromised mice. We observed variable outcomes with only one cell line showing a low level of glucose response. We, therefore, undertook a systematic comparison of different methods for inducing definitive endoderm and subsequently pancreatic differentiation. Of several protocols tested, we identified a combined approach that robustly generated pancreatic progenitors in vitro from both embryo-derived and induced pluripotent stem cells. These findings suggest that, although there are intrinsic differences in lineage specification propensity between pluripotent stem cell lines, optimal differentiation procedures may consistently direct a substantial fraction of cells into pancreatic specification.

scholarly journals Derivation of new human embryonic stem cell lines reveals rapid epigenetic progression in vitro that can be prevented by chemical modification of chromatin

2011 ◽  
Vol 21 (4) ◽  
pp. 751-764 ◽  
Author(s):  
Silvia V. Diaz Perez ◽  
Rachel Kim ◽  
Ziwei Li ◽  
Victor E. Marquez ◽  
Sanjeet Patel ◽  
...  
Keyword(s):  
Stem Cell ◽  
Chemical Modification ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Lines

Epigenetic regulation in stem cell development, cell fate conversion, and reprogramming

2015 ◽  
Vol 6 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Kazuyuki Ohbo ◽  
Shin-ichi Tomizawa
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Transcription Factors ◽  
Cell Fate ◽  
Cell Lines ◽  
Cell Fate Decisions ◽  
Micro Rnas ◽  
Stem Cell Lines ◽  
Cell Fate Conversion

AbstractStem cells are identified classically by an in vivo transplantation assay plus additional characterization, such as marker analysis, linage-tracing and in vitro/ex vivo differentiation assays. Stem cell lines have been derived, in vitro, from adult tissues, the inner cell mass (ICM), epiblast, and male germ stem cells, providing intriguing insight into stem cell biology, plasticity, heterogeneity, metastable state, and the pivotal point at which stem cells irreversibly differentiate to non-stem cells. During the past decade, strategies for manipulating cell fate have revolutionized our understanding about the basic concept of cell differentiation: stem cell lines can be established by introducing transcription factors, as with the case for iPSCs, revealing some of the molecular interplay of key factors during the course of phenotypic changes. In addition to de-differentiation approaches for establishing stem cells, another method has been developed whereby induced expression of certain transcription factors and/or micro RNAs artificially converts differentiated cells from one committed lineage to another; notably, these cells need not transit through a stem/progenitor state. The molecular cues guiding such cell fate conversion and reprogramming remain largely unknown. As differentiation and de-differentiation are directly linked to epigenetic changes, we overview cell fate decisions, and associated gene and epigenetic regulations.

scholarly journals SOX17 Is Not Required for the Derivation and Maintenance of Mouse Extraembryonic Endoderm Stem Cell Lines

2022 ◽  
Author(s):  
Xudong Dong ◽  
Ailing Ding ◽  
Jiangwei Lin
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Cell Lineage ◽  
Preimplantation Embryos ◽  
Primitive Endoderm ◽  
Extraembryonic Endoderm ◽  
Gene Expression Analyses ◽  
Stem Cell Lines

Extraembryonic endoderm stem (XEN) cell lines can be derived and maintained in vitro and reflect the primitive endoderm cell lineage. SOX17 is thought to be required for the derivation and maintenance of mouse XEN cell lines. Here we have re-evaluated this requirement for SOX17. We derived multiple SOX17-deficient XEN cell lines from preimplantation embryos of a SOX17-Cre knockout strain and chemically converted multiple SOX17-deficient embryonic stem cell lines into XEN cell lines by transient culturing with retinoic acid and Activin A. We confirmed the XEN profile of SOX17-deficient cell lines by immunofluorescence with various markers, by NanoString gene expression analyses, and by their contribution to the extraembryonic endoderm of chimeric embryos produced by injecting these cells into blastocysts. Thus, SOX17 is not required for the derivation and maintenance of XEN cell lines.

Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro

10.1038/74447 ◽  
2000 ◽  
Vol 18 (4) ◽  
pp. 399-404 ◽  
Author(s):  
Benjamin E. Reubinoff ◽  
Martin F. Pera ◽  
Chui-Yee Fong ◽  
Alan Trounson ◽  
Ariff Bongso
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Stem Cell Lines
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