E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display

2008 ◽  
Vol 3 (11) ◽  
pp. 1766-1777 ◽  
Author(s):  
Yariv Mazor ◽  
Thomas Van Blarcom ◽  
Brent L Iverson ◽  
George Georgiou
Keyword(s):  
Full Length ◽  
Igg Antibodies ◽  
Selection Of

scholarly journals Hospital-Based Donor Recruitment and Predonation Serologic Testing for COVID-19 Convalescent Plasma

2021 ◽  
Author(s):  
Joanna Balcerek ◽  
Evelin Trejo ◽  
Kendall Levine ◽  
Paul Couey ◽  
Zoe V Kornberg ◽  
...  
Keyword(s):  
Blood Donation ◽  
High Titer ◽  
Igg Antibodies ◽  
Serologic Testing ◽  
Wide Range ◽  
The Us ◽  
Polymerase Chain ◽  
Igg Level ◽  
Donor Recruitment ◽  
Selection Of

Abstract Objectives Serologic testing for antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in potential donors of coronavirus disease 2019 (COVID-19) convalescent plasma (CCP) may not be performed until after blood donation. A hospital-based recruitment program for CCP may be an efficient way to identify potential donors prospectively Methods Patients who recovered from known or suspected COVID-19 were identified and recruited through medical record searches and public appeals in March and April 2020. Participants were screened with a modified donor history questionnaire and, if eligible, were asked for consent and tested for SARS-CoV-2 antibodies (IgG and IgM). Participants positive for SARS-CoV-2 IgG were referred for CCP collection. Results Of 179 patients screened, 128 completed serologic testing and 89 were referred for CCP donation. IgG antibodies to SARS-CoV-2 were detected in 23 of 51 participants with suspected COVID-19 and 66 of 77 participants with self-reported COVID-19 confirmed by polymerase chain reaction (PCR). The anti–SARS-CoV-2 IgG level met the US Food and Drug Administration criteria for “high-titer” CCP in 39% of participants confirmed by PCR, as measured by the Ortho VITROS IgG assay. A wide range of SARS-CoV-2 IgG levels were observed. Conclusions A hospital-based CCP donor recruitment program can prospectively identify potential CCP donors. Variability in SARS-CoV-2 IgG levels has implications for the selection of CCP units for transfusion.

scholarly journals Immunogenicity and Neutralizing Activity Comparison of SARS-CoV-2 Spike Full-Length and Subunit Domain Proteins in Young Adult and Old-Aged Mice

Vaccines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 316
Author(s):  
Ki-Hye Kim ◽  
Noopur Bhatnagar ◽  
Subbiah Jeeva ◽  
Judy Oh ◽  
Bo Ryoung Park ◽  
...  
Keyword(s):  
Young Adult ◽  
Receptor Binding ◽  
Vaccine Dose ◽  
The Elderly ◽  
Full Length ◽  
High Dose ◽  
Igg Antibodies ◽  
Neutralizing Activity ◽  
Aged Mice ◽  
Adjuvant Effects

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to be expanding the pandemic disease across the globe. Although SARS-CoV-2 vaccines were rapidly developed and approved for emergency use of vaccination in humans, supply and production difficulties are slowing down the global vaccination program. The efficacy of many different versions of vaccine candidates and adjuvant effects remain unknown, particularly in the elderly. In this study, we compared the immunogenic properties of SARS-CoV-2 full-length spike (S) ectodomain in young adult and aged mice, S1 with receptor binding domain, and S2 with fusion domain. Full-length S was more immunogenic and effective in inducing IgG antibodies after low dose vaccination, compared to the S1 subunit. Old-aged mice induced SARS-CoV-2 spike-specific IgG antibodies with neutralizing activity after high dose S vaccination. With an increased vaccine dose, S1 was highly effective in inducing neutralizing and receptor-binding inhibiting antibodies, although both S1 and S2 subunit domain vaccines were similarly immunogenic. Adjuvant effects were significant for effective induction of IgG1 and IgG2a isotypes, neutralizing and receptor-binding inhibiting antibodies, and antibody-secreting B cell and interferon-γ secreting T cell immune responses. Results of this study provide information in designing SARS-CoV-2 spike vaccine antigens and effective vaccination in the elderly.

scholarly journals Recombinant MAG1 Protein of Toxoplasma gondii as a Diagnostic Antigen

2015 ◽  
Vol 64 (1) ◽  
pp. 55-59 ◽  
Author(s):  
JUSTYNA M. GATKOWSKA ◽  
BOŻENA DZIADEK ◽  
JAROSŁAW DZIADEK ◽  
KATARZYNA DZITKO ◽  
HENRYKA DŁUGOŃSKA
Keyword(s):  
Toxoplasma Gondii ◽  
Full Length ◽  
Specific Marker ◽  
Igg Antibodies ◽  
Diagnostic Antigen ◽  
Human Sera ◽  
Diagnostic Usefulness ◽  
Native Antigen

The aim of this study was to evaluate the potential diagnostic usefulness of the full-length recombinant Toxoplasma gondii MAG1 protein by determining the levels of specific IgM and IgG antibodies in mouse and human sera obtained from individuals with acute and chronic toxoplasmosis. The obtained results revealed that IgG antibodies against MAG1 are a sensitive and specific marker of T. gondii infection since the protein was recognized by both mouse and human sera, 100% and 94.3%, respectively, rendering the full-length rMAG1 a prospective alternative for the polyvalent native antigen (TLA).

Webb, Phyllis (1927– )

2018 ◽  
Author(s):  
Melissa Dalgleish
Keyword(s):  
British Columbia ◽  
West Coast ◽  
Full Length ◽  
The West ◽  
University Of British Columbia ◽  
Mcgill University ◽  
Phyllis Webb ◽  
The University ◽  
Point Of Departure ◽  
Selection Of

Phyllis Webb, OC is a Canadian poet, teacher, and broadcaster. She was born in Victoria, British Columbia and attended the University of British Columbia and McGill University. She is the author of numerous books of poetry, a selection of prose, and a collection of broadcast scripts, essays and reviews published as Talking (1982). Her first collection of poems was published alongside work by Eli Mandel and Gael Turnbull in Trio (1954). Webb’s first full-length collection, Even Your Right Eye (1956) was followed by The Sea is Also a Garden (1962). She began working at CBC Toronto in 1964 and acted as the producer of the ‘Ideas’ programme from 1967–1969. Her 1965 collection Naked Poems marked a point of departure with its compact forms and erotic evocation of lesbian desire. After returning to the West Coast, Webb did not publish another full-length collection until Wilson’s Bowl (1980). She won the Governor General’s Award for The Vision Tree (1982). Webb’s interest in the Persian ghazal form inspired Sunday Water: Thirteen Anti-Ghazals (1982) and Water and Light: Ghazals and Anti-Ghazals (1984). Her consistent concern with form manifests itself in her formal experimentation and her meticulous crafting of poems.

scholarly journals Death and Coping Mechanisms in Animated Disney Movies: A Content Analysis of Disney Films (1937–2003) and Disney/Pixar Films (2003–2016)

2018 ◽  
Vol 7 (10) ◽  
pp. 199 ◽  
Author(s):  
James A. Graham ◽  
Hope Yuhas ◽  
Jessica L. Roman
Keyword(s):  
Content Analysis ◽  
Coping Mechanisms ◽  
Emotional Reaction ◽  
Original Study ◽  
Full Length ◽  
The Past ◽  
Animated Films ◽  
And Coping ◽  
Selection Of

The purpose of this content analysis was to examine how death depictions in animated Disney films has changed in the past 14 years and the coping mechanisms used to process death within these films. A content analysis from 2005 was used to investigate the influence of Disney films on children’s concepts of death based on 23 death scenes from 10 full-length Disney Classic animated films from 1937 to 2003 and 10 death scenes from 8 selected full-length Disney and Pixar animated films from 2003 to 2016. Our goal was to compare the findings across the two studies. Similar to the original study, the portrayal of death focused on five categories: character status; depiction of death; death status; emotional reaction; and causality. We expanded on the original study and more research by examining coping mechanisms used to process death within a selection of these films. Our findings indicated that some scenes from animated Disney and Pixar films obscure the permanence and irreversibility of death and often fail to acknowledge deaths emotionally. Our conclusions showed that Disney’s and Pixar’s portrayal of death in newer films might have more positive implications for children’s understanding of death than Disney Classic animated films.

scholarly journals Modified Vaccinia Ankara Based SARS-CoV-2 Vaccine Expressing Full-Length Spike Induces Strong Neutralizing Antibody Response

2020 ◽  
Author(s):  
Nanda Kishore Routhu ◽  
Sailaja Gangadhara ◽  
Narayanaiah Cheedarla ◽  
Ayalnesh Shiferaw ◽  
Sheikh Abdul Rahman ◽  
...  
Keyword(s):  
Antibody Response ◽  
Room Temperature ◽  
Vaccine Candidate ◽  
Neutralizing Antibody ◽  
Antibody Responses ◽  
Full Length ◽  
Spike Protein ◽  
Strongly Correlated ◽  
Igg Antibodies ◽  
Potential Vaccine

AbstractThere is a great need for the development of vaccines for preventing SARS-CoV-2 infection and mitigating the COVID-19 pandemic. Here, we developed two modified vaccinia Ankara (MVA) based vaccines which express either a membrane anchored full-length spike protein (MVA/S) stabilized in a prefusion state or the S1 region of the spike (MVA/S1) which forms trimers and is secreted. Both immunogens contained the receptor-binding domain (RBD) which is a known target of antibody-mediated neutralization. Following immunizations with MVA/S or MVA/S1, both spike protein recombinants induced strong IgG antibodies to purified full-length SARS-CoV-2 spike protein. The MVA/S induced a robust antibody response to purified RBD, S1 and S2 whereas MVA/S1 induced an antibody response to the S1 region outside of the RBD region. Both vaccines induced an antibody response in the lung and that was associated with induction of bronchus-associated lymphoid tissue. MVA/S but not MVA/S1 vaccinated mice generated robust neutralizing antibody responses against SARS-CoV-2 that strongly correlated with RBD antibody binding titers. Mechanistically, S1 binding to ACE-2 was strong but reduced following prolonged pre-incubation at room temperature suggesting confirmation changes in RBD with time. These results demonstrate MVA/S is a potential vaccine candidate against SARS-CoV-2 infection.

scholarly journals Positive selection of ORF3a and ORF8 genes drives the evolution of SARS-CoV-2 during the 2020 COVID-19 pandemic

2020 ◽  
Author(s):  
Lauro Velazquez-Salinas ◽  
Selene Zarate ◽  
Samantha Eberl ◽  
Douglas P. Gladue ◽  
Isabel Novella ◽  
...  
Keyword(s):  
Public Health ◽  
Positive Selection ◽  
Evolutionary Dynamics ◽  
Full Length ◽  
Accessory Proteins ◽  
The Novel ◽  
Phylogenetic Groups ◽  
Epistatic Interactions ◽  
Genetic Mechanisms ◽  
Selection Of

AbstractIn this study, we analyzed full-length SARS-CoV-2 genomes from multiple countries to determine early trends in the evolutionary dynamics of the novel COVID-19 pandemic. Results indicated SARS-CoV-2 evolved early into at least three phylogenetic groups, characterized by positive selection at specific residues of the accessory proteins OFR3a and ORF8a. We also report evidence of epistatic interactions among sites in the genome that may be important in the generation of variants adapted to humans. These observations might impact not only public health, but also suggest more studies are needed to understand the genetic mechanisms that may affect the development of therapeutic and preventive tools, like antivirals and vaccines.

scholarly journals Dominant Epitopes of the C6 Diagnostic Peptide of Borrelia burgdorferi Are Largely Inaccessible to Antibody on the Parent VlsE Molecule

2007 ◽  
Vol 14 (8) ◽  
pp. 931-936 ◽  
Author(s):  
Monica E. Embers ◽  
Mary B. Jacobs ◽  
Barbara J. B. Johnson ◽  
Mario T. Philipp
Keyword(s):  
Antibody Response ◽  
Variable Region ◽  
Immunoglobulin M ◽  
Antibody Responses ◽  
Full Length ◽  
Molecular Surface ◽  
Surface Molecule ◽  
Igg Antibodies ◽  
Specific Antibodies ◽  
Variable Surface

ABSTRACTLyme borreliosis (LB) is a disease for which antibody-based detection assays are often required for diagnosis. The variable surface molecule VlsE and IR6, one of its invariable regions, are commonly targeted by the antibody response in infected individuals. A series of enzyme-linked immunosorbent assays was performed to comparatively examine the antibody responses of North American LB patients (n= 37) to VlsE and invariable segments of this molecule. Both immunoglobulin M (IgM) and IgG responses to full-length VlsE and to peptides reproducing invariable regions 2, 4, and 6, as well as the invariable domains at the amino and carboxyl termini of VlsE, were assessed. The proportions and specificities of reactivity to the invariable segments were tested by using cognate peptides as competitors for VlsE binding by patient serum antibodies. IR6 epitopes (by the C6 peptide) were found to dominate the response to invariable segments. IR6 (C6)-specific antibodies were detected in 78% of the serum specimens, whereas <40% of patients generated antibodies that bound the N- or C-terminal domain and <12% of patients responded to either IR2 or IR4. Interestingly, 15 of 37 patients generated IgG antibodies that reacted with C6 but not with VlsE. Conversely, IgM responses were frequent for VlsE but not for invariable segments. A representative number of the serum specimens (n= 8) that contained IgG antibodies reacting with both C6 and VlsE was assessed in competition experiments, using C6 as a competitor. Only half of these specimens contained IgG antibodies whose binding to VlsE could be inhibited >50% by competition with the added C6 peptide. The median percent inhibition was 45.5%. These findings indicate that IR6 epitopes are largely concealed from the VlsE molecular surface and that full-length VlsE-based diagnosis likely detects antibodies to conformational and/or variable region epitopes.

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