High-accuracy neurite reconstruction for high-throughput neuroanatomy

2011 ◽  
Vol 14 (8) ◽  
pp. 1081-1088 ◽  
Author(s):  
Moritz Helmstaedter ◽  
Kevin L Briggman ◽  
Winfried Denk
Keyword(s):  
High Throughput ◽  
High Accuracy

scholarly journals SpyCLIP: an easy-to-use and high-throughput compatible CLIP platform for the characterization of protein–RNA interactions with high accuracy

2019 ◽  
Vol 47 (6) ◽  
pp. e33-e33 ◽  
Author(s):  
Ya Zhao ◽  
Yao Zhang ◽  
Yilan Teng ◽  
Kai Liu ◽  
Yanqing Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
High Accuracy

scholarly journals Efficient anchoring of Erianthus arundinaceus chromatin introgressed into sugarcane by specific molecular markers

2020 ◽  
Author(s):  
Yongji Huang ◽  
Jiayun Wu ◽  
Xueting Li ◽  
Fan Yu ◽  
Xuguang Hu ◽  
...  
Keyword(s):  
Molecular Markers ◽  
High Throughput ◽  
Repetitive Sequence ◽  
Repetitive Sequences ◽  
Distribution Patterns ◽  
Subtractive Hybridization ◽  
High Accuracy ◽  
Wild Relatives ◽  
45S Rdna ◽  
Erianthus Arundinaceus

Abstract Erianthus arundinaceus is a valuable gene reservoir for sugarcane improvement. However, insufficient molecular markers for high-accuracy identification and tracking of the introgression status of E. arundinaceus chromatin impede sugarcane breeding. Fortunately, suppression subtractive hybridization (SSH) technology provides an excellent opportunity for development of high-throughput E. arundinaceus-specific molecular markers at a reasonable cost. In this study, we constructed a SSH library of E. arundinaceus. In total, 288 clones E. arundinaceus-specific repetitive sequences were screened out and their distribution patterns on chromosomes were characterized by fluorescence in situ hybridization (FISH). A subtelomeric repetitive sequence Ea086 and a diffusive repetitive sequence Ea009, plus 45S rDNA-bearing E. arundinaceus chromosome repetitive sequence EaITS were developed as E. arundinaceus-specific molecular markers, namely Ea086-128, Ea009-257, and EaITS-278, covering all the E. arundinaceus chromosomes for high-accuracy identification of putative progeny. Both Ea086-128 and Ea009-257 were successfully applied to identify the authenticity of F1, BC1, BC2, BC3, and BC4 progeny between sugarcane and E. arundinaceus. In addition, EaITS-278 was a 45S rDNA-bearing E. arundinaceus chromosome-specific molecular marker for rapid tracking the inherited status of this chromosome in sugarcane background. Three BC3 progeny had apparently lost the 45S rDNA-bearing E. arundinaceus chromosome. We reported herein a highly effective and reliable SSH-based technology for discovery of high-throughput E. arundinaceus-specific sequences bearing high potential as molecular markers. Given its reliability and savings in time and efforts, the method is also suitable for development of species-specific molecular markers for other important wild relatives to accelerate introgression of wild relatives into sugarcane.

scholarly journals Microarcsecond Astrometric Interferometry

1986 ◽  
Vol 109 ◽  
pp. 321-330
Author(s):  
Robert D. Reasenberg
Keyword(s):  
High Throughput ◽  
High Accuracy ◽  
Observation Time ◽  
Mission Design ◽  
Photon Statistics ◽  
Design Criteria ◽  
Optical Interferometer ◽  
Laser Interferometers ◽  
Scientific Questions ◽  
Comparable Size

A small astrometric optical interferometer, Mini-POINTS, which would fit fully assembled in about one-third of the Shuttle bay, could measure the angle between stars about 90° apart. For 10th magnitude stars, an observation time of about 20 minutes would yield a measurement uncertainty of 5 microarcseconds. When compared to an astrometric telescope of comparable size which observes the same target for the same period of time, such an interferometer would achieve a greater accuracy by two to three orders of magnitude. Five design criteria lead to an instrument that achieves high precision by employing photon statistics for fringe splitting and achieves high accuracy by means of continuous internal metrology with laser interferometers. The high throughput of Mini-POINTS permits a mission design that addresses a wide variety of scientific questions.

scholarly journals High-throughput, high-accuracy array-based resequencing

2009 ◽  
Vol 106 (16) ◽  
pp. 6712-6717 ◽  
Author(s):  
J. Zheng ◽  
M. Moorhead ◽  
L. Weng ◽  
F. Siddiqui ◽  
V. E. H. Carlton ◽  
...  
Keyword(s):  
High Throughput ◽  
High Accuracy

scholarly journals A novel high-accuracy genome assembly method utilizing a high-throughput workflow

2020 ◽  
Author(s):  
Qingdong Zeng ◽  
Wenjin Cao ◽  
Liping Xing ◽  
Guowei Qin ◽  
Jianhui Wu ◽  
...  
Keyword(s):  
High Throughput ◽  
Genome Assembly ◽  
Large Scale ◽  
Sequence Data ◽  
Physical Map ◽  
Bac Library ◽  
High Accuracy ◽  
Biological Research ◽  
Bac Clone ◽  
Assembly Method

AbstractAcross domains of biological research using genome sequence data, high-quality reference genome sequences are essential for characterizing genetic variation and understanding the genetic basis of phenotypes. However, the construction of genome assemblies for various species is often hampered by complexities of genome organization, especially repetitive and complex sequences, leading to mis-assembly and missing regions. Here, we describe a high-throughput gold standard genome assembly workflow using a large-scale bacterial artificial chromosome (BAC) library with a refined two-step pooling strategy and the Lamp assembler algorithm. This strategy minimizes the laborious processes of physical map construction and clone-by-clone sequencing, enabling inexpensive sequencing of several thousand BAC clones. By applying this strategy with a minimum tiling path BAC clone library for the short arm of chromosome 2D (2DS) of bread wheat, 98% of BAC sequences, covering 92.7% of the 2DS chromosome, were assembled correctly for this species with a highly complex and repetitive genome. We also identified 48 large mis-assemblies in the reference wheat genome assembly (IWGSC RefSeq v1.0) and corrected these large mis-assemblies in addition to filling 92.2% of the gaps in RefSeq v1.0. Our 2DS assembly represents a new benchmark for the assembly of complex genomes with both high accuracy and efficiency.

scholarly journals Efficient anchoring of Erianthus arundinaceus chromatin introgressed into sugarcane by specific molecular markers

2020 ◽  
Author(s):  
Yongji Huang ◽  
Jiayun Wu ◽  
Xueting Li ◽  
Fan Yu ◽  
Xuguang Hu ◽  
...  
Keyword(s):  
Molecular Markers ◽  
High Throughput ◽  
Repetitive Sequence ◽  
Repetitive Sequences ◽  
Distribution Patterns ◽  
Subtractive Hybridization ◽  
High Accuracy ◽  
Wild Relatives ◽  
45S Rdna ◽  
Species Specific

Abstract Background: Erianthus arundinaceus is a valuable gene reservoir for sugarcane improvement. However, insufficient molecular markers for high-accuracy identification and tracking of the introgression status of E. arundinaceus chromatin impede sugarcane breeding. Fortunately, suppression subtractive hybridization (SSH) technology provides an excellent opportunity for development of high-throughput E. arundinaceus-specific molecular markers at a reasonable cost. Results: In this study, we constructed a SSH library of E. arundinaceus. In total, 288 clones E. arundinaceus-specific repetitive sequences were screened out and their distribution patterns on chromosomes were characterized by fluorescence in situ hybridization (FISH). A subtelomeric repetitive sequence Ea086 and a diffusive repetitive sequence Ea009, plus 45S rDNA-bearing E. arundinaceus chromosome repetitive sequence EaITS were developed as E. arundinaceus-specific molecular markers, namely Ea086-128, Ea009-257, and EaITS-278, covering all the E. arundinaceus chromosomes for high-accuracy identification of putative progeny. Both Ea086-128 and Ea009-257 were successfully applied to identify the authenticity of F1, BC1, BC2, BC3, and BC4 progeny between sugarcane and E. arundinaceus. In addition, EaITS-278 was a 45S rDNA-bearing E. arundinaceus chromosome-specific molecular marker for rapid tracking the inherited status of this chromosome in sugarcane background. Three BC3 progeny had apparently lost the 45S rDNA-bearing E. arundinaceus chromosome. Conclusions: We reported herein a highly effective and reliable SSH-based technology for discovery of high-throughput E. arundinaceus-specific sequences bearing high potential as molecular markers. Given its reliability and savings in time and efforts, the method is also suitable for development of species-specific molecular markers for other important wild relatives to accelerate introgression of wild relatives into sugarcane.

scholarly journals Hybrid microfluidic sorting of rare cells based on high throughput inertial focusing and high accuracy acoustic manipulation

RSC Advances ◽  
2019 ◽  
Vol 9 (53) ◽  
pp. 31186-31195 ◽  
Author(s):  
Yinning Zhou ◽  
Zhichao Ma ◽  
Ye Ai
Keyword(s):  
High Throughput ◽  
High Accuracy ◽  
Cell Populations ◽  
Inertial Focusing ◽  
Fold Enrichment ◽  
Rare Cells ◽  
Microfluidic Sorting

The first example of integration of sized-based inertial sorting and surface biomarker-based acoustic sorting to achieve >2500-fold enrichment of rare cell populations.

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