scholarly journals Localization of inner hair cell mechanotransducer channels using high-speed calcium imaging

2009 ◽  
Vol 12 (5) ◽  
pp. 553-558 ◽  
Author(s):  
Maryline Beurg ◽  
Robert Fettiplace ◽  
Jong-Hoon Nam ◽  
Anthony J Ricci
Keyword(s):  
Hair Cell ◽  
Calcium Imaging ◽  
High Speed ◽  
Inner Hair Cell

scholarly journals In situ motions of individual inner-hair-cell stereocilia from stapes stimulation in adult mice

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yanli Wang ◽  
Charles R. Steele ◽  
Sunil Puria ◽  
Anthony J. Ricci
Keyword(s):  
Hair Cell ◽  
High Speed ◽  
Ex Vivo ◽  
Ionic Currents ◽  
Hair Bundle ◽  
Sensory Cells ◽  
Apical Surface ◽  
Inner Hair Cell ◽  
High Speed Imaging

AbstractIn vertebrate hearing organs, mechanical vibrations are converted to ionic currents through mechanoelectrical-transduction (MET) channels. Concerted stereocilia motion produces an ensemble MET current driving the hair-cell receptor potential. Mammalian cochleae are unique in that the tuning of sensory cells is determined by their mechanical environment and the mode of hair-bundle stimulation that their environment creates. However, little is known about the in situ intra-hair-bundle motions of stereocilia relative to one another, or to their environment. In this study, high-speed imaging allowed the stereocilium and cell-body motions of inner hair cells to be monitored in an ex vivo organ of Corti (OoC) mouse preparation. We have found that the OoC rotates about the base of the inner pillar cell, the hair bundle rotates about its base and lags behind the motion of the apical surface of the cell, and the individual stereocilia move semi-independently within a given hair bundle.

scholarly journals Inner hair cell stereocilia are embedded in the tectorial membrane

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pierre Hakizimana ◽  
Anders Fridberger
Keyword(s):  
Electron Microscopy ◽  
Hair Cell ◽  
Mechanical Stimulation ◽  
Hair Cells ◽  
Viscous Drag ◽  
Tectorial Membrane ◽  
Inner Hair Cell ◽  
Inner Hair Cells ◽  
Inward Currents

AbstractMammalian hearing depends on sound-evoked displacements of the stereocilia of inner hair cells (IHCs), which cause the endogenous mechanoelectrical transducer channels to conduct inward currents of cations including Ca2+. Due to their presumed lack of contacts with the overlaying tectorial membrane (TM), the putative stimulation mechanism for these stereocilia is by means of the viscous drag of the surrounding endolymph. However, despite numerous efforts to characterize the TM by electron microscopy and other techniques, the exact IHC stereocilia-TM relationship remains elusive. Here we show that Ca2+-rich filamentous structures, that we call Ca2+ ducts, connect the TM to the IHC stereocilia to enable mechanical stimulation by the TM while also ensuring the stereocilia access to TM Ca2+. Our results call for a reassessment of the stimulation mechanism for the IHC stereocilia and the TM role in hearing.

Free radical scavenger protects against inner hair cell loss after cochlear ischemia

Neuroreport ◽  
2003 ◽  
Vol 14 (14) ◽  
pp. 1881-1884 ◽  
Author(s):  
Toshiki Maetani ◽  
Nobuhiro Hakuba ◽  
Masafumi Taniguchi ◽  
Jun Hyodo ◽  
Yoshitaka Shimizu ◽  
...  
Keyword(s):  
Free Radical ◽  
Hair Cell ◽  
Cell Loss ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Hair Cell Loss ◽  
Inner Hair Cell

scholarly journals A Complex of RIM2alpha and RIM-Binding Protein 2 Stabilizes Slow Voltage-Dependent Inactivation of Cochlear Inner Hair Cell Cav1.3 L-Type Ca2+ Channels

2018 ◽  
Vol 114 (3) ◽  
pp. 637a-638a
Author(s):  
Nadine J. Ortner ◽  
Alexandra Pinggera ◽  
Anita Siller ◽  
Nadja Hofer ◽  
Niels Brandt ◽  
...  
Keyword(s):  
Hair Cell ◽  
Binding Protein ◽  
Inner Hair Cell ◽  
Dependent Inactivation ◽  
Voltage Dependent ◽  
Ca2 Channels
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