scholarly journals Inner hair cell stereocilia movements captured in-situ by a high-speed camera with subpixel image processing

Author(s):  
Yanli Wang ◽  
Sunil Puria ◽  
Charles R. Steele ◽  
Anthony J. Ricci
2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yanli Wang ◽  
Charles R. Steele ◽  
Sunil Puria ◽  
Anthony J. Ricci

AbstractIn vertebrate hearing organs, mechanical vibrations are converted to ionic currents through mechanoelectrical-transduction (MET) channels. Concerted stereocilia motion produces an ensemble MET current driving the hair-cell receptor potential. Mammalian cochleae are unique in that the tuning of sensory cells is determined by their mechanical environment and the mode of hair-bundle stimulation that their environment creates. However, little is known about the in situ intra-hair-bundle motions of stereocilia relative to one another, or to their environment. In this study, high-speed imaging allowed the stereocilium and cell-body motions of inner hair cells to be monitored in an ex vivo organ of Corti (OoC) mouse preparation. We have found that the OoC rotates about the base of the inner pillar cell, the hair bundle rotates about its base and lags behind the motion of the apical surface of the cell, and the individual stereocilia move semi-independently within a given hair bundle.


2021 ◽  
Vol 129 (18) ◽  
pp. 183305
Author(s):  
Mário Janda ◽  
Mostafa E. Hassan ◽  
Viktor Martišovitš ◽  
Karol Hensel ◽  
Michal Kwiatkowski ◽  
...  

2012 ◽  
Vol 160 ◽  
pp. 77-81
Author(s):  
Jing Jing Tian ◽  
Lei Han

Kick-up phenomenon during looping is an important factor in thermosonic wire bonding. In this study, the loping process during wire bonding was recorded by using high-speed camera, and wire profiles evolution was obtained from images sequence by image processing method. With a polynomial fitting, the wire loop profiling was described by the curvature changing, and kick-up phenomenon on gold wire was found between the instant of 290th frame(0.0537s) to 380th frame (0.0703s), the change of curvature is divided into three phases, a looping phase, a mutation phase and a kick-up phase. While in the kick-up phase, the kick up phenomenon is the most obvious. These experimental results were useful for in-depth study of kick-up phenomenon by simulation.


2012 ◽  
Vol 455-456 ◽  
pp. 1140-1144
Author(s):  
Zhi Guo Luo ◽  
Li Hao Han ◽  
Xiao Lei ◽  
Zhan Xia Di ◽  
Jun Jie Sun

In this paper, a two-dimensional hot model of melter gasifier, in which paraffin and corn are used to simulate DRI, coke and lump coal respectively, has been established to study the regional boundary in this paper. While the whole experimental process is recorded by the high-speed camera, the image processing method is put forward to define each regional boundary. By means of this method, the boundary of raceway, cohesive zone can be obtained quantificationally.


2008 ◽  
Vol 94 (11) ◽  
pp. 532-538 ◽  
Author(s):  
Kazumi Mizukami ◽  
Masaaki Sugiyama ◽  
Masaharu Tsuji

2021 ◽  
Vol 29 (1(145)) ◽  
pp. 35-39
Author(s):  
Volkan Kaplan

Warp tensions were measured while a machine was operating on a woven cotton fabric with three different woven patterns. This study was carried out with image analysis methods using a high speed camera. Three weave pattern types: plain, twill and satin were woven on the same weaving machine, and thus it could be understood how weave pattern differences affect warp tension. Each of these three weaves was woven in three weft densities: 20, 28 and 45 wefts per cm. These fabrics were able to be made on a weaving machine with an automatic dobby. It was aimed to investigate warp tension differences for three basic weave patterns while keeping all machine settings constant. The weave settings of the dobby were changed for plain, twill and satin weaves. Warp tension calculation was based on the warp elasticity theory. Warp elasticises were measured by image processing methods in MATLAB using a high-speed camera. It was aimed to improve upon the new method of warp extension measurement of fabric when the loom is in operation. It was observed that the warp tension in plain fabric was higher than for twill and satin under the same conditions.


Sign in / Sign up

Export Citation Format

Share Document